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Type IV Collagen Promotes Adipogenic Differentiation of Adipose Stem Cells

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Abstract

Type IV collagen is a major component of the extracellular matrix in adipose tissue. It is secreted during the lipogenic differentiation of mesenchymal stem cells, but its direct impact and mechanism on the differentiation of adipose-derived stem cells (ASCs) into lipids are unclear. In this study, ASCs were obtained from human liposuction samples and cultured. Lipogenic induction of ASCs was achieved using lipogenic induction medium. Immunofluorescence analysis revealed differential expression of type IV collagen during the early and late stages of adipogenic induction, displaying a distinct morphological encapsulation of ASCs. Silencing of type IV collagen using siRNA resulted in a significant decrease in adipogenic capacity, as indicated by reduced lipid droplet formation and downregulation of adipogenic-related gene transcription. Conversely, supplementation of the culture medium with synthetic type IV collagen demonstrated enhanced adipogenic induction efficiency, accompanied by upregulation of YAP/TAZ protein expression and its downstream target gene transcription. Furthermore, inhibition of the YAP/TAZ pathway using the inhibitor Blebbistatin attenuated the functionality of type IV collagen, leading to decreased lipid droplet formation and downregulation of adipocyte maturation-related gene expression. These findings highlight the crucial role of type IV collagen in promoting adipogenic differentiation of ASCs and suggest its involvement in the YAP/TAZ-mediated Hippo pathway.

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Funding

This work was supported by the National Nature Science Foundation of China (81801932, 81901976, 81971852, 81772101, 81901975)

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Author notes

  1. Jian Li, Yidan Xu and Yuping Quan have equally contributed to this work and should be considered co-first authors.

Authors and Affiliations

  1. Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, 510515, Guangdong, People’s Republic of China

    Jian Li, Yidan Xu, Yufei He, Feng Lu, Jianhua Gao, Yao Yao & Yunjun Liao

  2. Department of Plastic Surgery and Regenerative Medicine, Fujian Medical University Union Hospital, Fuzhou, 350 001, People’s Republic of China

    Yuping Quan

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  1. Jian Li
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Correspondence to Jianhua Gao, Yao Yao or Yunjun Liao.

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Li, J., Xu, Y., Quan, Y. et al. Type IV Collagen Promotes Adipogenic Differentiation of Adipose Stem Cells. Aesth Plast Surg (2024). https://doi.org/10.1007/s00266-024-03890-w

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