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Effects of Oleuropein Oral Intake on Infected Fat Grafts: Experimental Study

  • Original Articles
  • Basic Science/Experimental
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Abstract

Aim

The aim of this study was to evaluate the effects of oleuropein oral intake on infected fat grafts and fat graft survival.

Materials and Methods

32 Wistar albino rats were divided into four groups: 0. none treated, 1. serum oral intake, 2. antibiotic oral intake, and 3. oleuropein oral intake. The dorsal regions of the rats were separated into four quadrants as right and left cranial, and right and left caudal to determine each quadrant where fat grafts were placed. Right cranial and caudal quadrants were infected with the methicillin-susceptible Staphylococcus aureus (MSSA) strain. The left cranial and caudal quadrants were infected with the Pseudomonas aeruginosa strain. On the 7th day and end of the 3rd month, fibroblast density, inflammation, and fat survival were demonstrated immunohistochemically with FGF, CD68, and perilipin (PP), respectively.

Results

On the 7th day, for P. aureginosa-infected grafts, oleuropein was shown higher rates in CD68 and PP staining compared to the antibiotic group (p < 0.05, p < 0.001, respectively). At the end of the 3rd month, for P. aureginosa and S. aureus-infected grafts, the oleuropein group was demonstrated improved PP staining rates compared to the antibiotic group (p < 0.01, p < 0.01, respectively).

Conclusion

Oleuropein as a natural olive leaf extract with potent antioxidant, anti-inflammatory, and antimicrobial features is an alternative and supportive agent for both treatment and prophylaxis of surgical site infections like the antibiotics of chemical synthesis. P. aeruginosa and S. aureus surgical site infections could treat and prevent safely and effectively by oleuropein, particularly in early and late periods after surgery.

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References

  1. Castejón ML, Montoya T, Alarcón-de-la-Lastra C, Sánchez-Hidalgo M (2020) Potential protective role exerted by secoiridoids from olea Europaea L. in cancer, cardiovascular, neurodegenerative, aging-related, and immunoinflammatory diseases. Antioxidants 9:149

    Article  PubMed  PubMed Central  Google Scholar 

  2. Ghomari O, Sounni F, Massaoudi Y, Ghanam J, Kaitouni LBD, Merzouki M, Benlemlih M (2019) Phenolic profile (HPLC-UV) of olive leaves according to extraction procedure and assessment of antibacterial activity. Biotechnol Rep 23:e00347

    Article  Google Scholar 

  3. Fuglestad MA, Tracey EL, Leinicke JA (2021) Evidence-based prevention of surgical site infection. Surg Clin North Am 101(6):951–966. https://doi.org/10.1016/j.suc.2021.05.027

    Article  PubMed  Google Scholar 

  4. Gutowski KA (2009) Current applications and safety of autologous fat grafts: a report of the ASPS fat graft task force: ASPS fat graft task force. Plast Reconstr Surg 124:272–280

    Article  CAS  PubMed  Google Scholar 

  5. Pradeep MSS, Rao KVV (2019) A study on surgical Site Infections, their bacteriological profile and antimicrobial susceptibility pattern. Int J Med Microbiol Trop Dis 5(1):9–13

    Google Scholar 

  6. Okyay MF, Oztermeli A (2023) Evaluation of the effect of metoprolol dosage on fat graft survival. Aesthetic Plast Surg. https://doi.org/10.1007/s00266-023-03271-9

    Article  PubMed  Google Scholar 

  7. Andrews JM (2001) Determination of minimum inhibitory concentrations. J Antimicrob Chemother 48:5–16

    Article  CAS  PubMed  Google Scholar 

  8. Goodpaster T, Legesse-Miller A, Hameed MR, Aisner SC, Randolph-Habecker J, Coller HA (2008) An immunohistochemical method for identifying fibroblasts in formalin-fixed, paraffin-embedded tissue. J Histochem Cytochem 56(4):347–358. https://doi.org/10.1369/jhc.7A7287.2007

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Lloyd CM, Phillips AR, Cooper GJ, Dunbar PR (2008) Three-colour fluorescence immunohistochemistry reveals the diversity of cells staining for macrophage markers in murine spleen and liver. J Immunol Meth 334(1–2):70–81. https://doi.org/10.1016/j.jim.2008.02.005

    Article  CAS  Google Scholar 

  10. Pham NA, Morrison A, Schwock J, Aviel-Ronen S, Iakovlev V, Tsao MS, Ho J, Hedley DW (2007) Quantitative image analysis of immunohistochemical stains using a CMYK color model. Diagn Pathol 27(2):8. https://doi.org/10.1186/1746-1596-2-8

    Article  CAS  Google Scholar 

  11. Asserson DB, Kulinich AG, Orbay H, Sahar DE (2019) Differences in complication rates of gluteoplasty procedures that utilize autologous fat grafting, implants, or local flaps. Ann Plast Surg 82:342–344

    Article  Google Scholar 

  12. Schiraldi L, Sapino G, Meuli J, Maruccia M, Cherubino M, Raffoul W, di Summa PG (2022) Facial fat grafting (FFG): worth the risk? A systematic review of complications and critical appraisal. J Clin Med 11:4708

    Article  PubMed  PubMed Central  Google Scholar 

  13. Hyakusoku H, Ogawa R, Ono S, Ishii N, Hirakawa K (2009) Complications after autologous fat injection to the breast. Plast Reconstr Surg 123(1):360–370. https://doi.org/10.1097/PRS.0b013e31819347ba

    Article  CAS  PubMed  Google Scholar 

  14. Cuevas Bustos R, Cervantes Gutierrez O, Perez Tristan FA, AcuñaMacouzet A, Flores-Huidobro Martinez A, JafifCojab M (2020) Necrotizing soft tissue infection after liposculpture; case report. Int J Surg Case Rep. 77:677–681. https://doi.org/10.1016/j.ijscr.2020.11.078

    Article  PubMed  PubMed Central  Google Scholar 

  15. Yu NZ, Huang JZ, Zhang H, Wang Y, Wang XJ, Zhao R, Bai M, Long X (2015) A systemic review of autologous fat grafting survival rate and related severe complications. Chin Med J (Engl) 128(9):1245–1251. https://doi.org/10.4103/0366-6999.156142

    Article  PubMed  Google Scholar 

  16. Smith OJ, Esmaeili A, Mosahebi A (2018) Use of prophylactic antibiotics in fat grafting and their effect on graft site infection. Aesthet Surg J 38(8):NP118–NP119. https://doi.org/10.1093/asj/sjy123

    Article  PubMed  Google Scholar 

  17. Li X, Liu Y, Jia Q, LaMacchia V, O’Donoghue K, Huang Z (2016) A systems biology approach to investigate the antimicrobial activity of oleuropein. J Ind Microbiol Biotechnol 43(12):1705–1717. https://doi.org/10.1007/s10295-016-1841-8

    Article  CAS  PubMed  Google Scholar 

  18. Qabaha K, Al-Rimawi F, Qasem A, Naser SA (2018) Oleuropein Is responsible for the major anti-inflammatory effects of olive leaf extract. J Med Food 21:302–305

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Hatay Mustafa Kemal University Experimental Research Application and Research Center, 31000, Hatay, Turkey

    Hamdullah Suphi Bayraktar

  2. Dr. MFO Clinic, Antalya, Turkey

    Mehmet Fatih Okyay

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  1. Hamdullah Suphi Bayraktar
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  2. Mehmet Fatih Okyay
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Correspondence to Hamdullah Suphi Bayraktar.

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The authors declare that they have no conflict of interest.

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Ethical permission was obtained from the Hatay Mustafa Kemal University Animal Experiments Local Ethics Committee with decision number 2021/02-09. All applicable institutional and/or national guidelines for the care and use of animals were followed.

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Bayraktar, H.S., Okyay, M.F. Effects of Oleuropein Oral Intake on Infected Fat Grafts: Experimental Study. Aesth Plast Surg 48, 1218–1228 (2024). https://doi.org/10.1007/s00266-023-03612-8

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  • DOI: https://doi.org/10.1007/s00266-023-03612-8

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