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Effectiveness of 5-Fluorouracil and Hypertonic Glucose in the Prevention of Post-mastectomy Seroma in an Experimental Animal Model

  • Original Articles
  • Basic Science/Experimental
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Abstract

Introduction

Seroma is a frequent complication that can affect the final result of reconstructive and cosmetic surgeries.

Methodology

This study evaluated the effectiveness of 5-Fluorouracil and 75% hypertonic glucose in preventing seroma in a mastectomy rat model, as well as cellular and vascular events in adjacent tissues. A left mastectomy with lymphadenectomy was performed in 60 Wistar-Albino female rats. Animals randomly allocated to the control group (Group I; n = 20) were sutured right after mastectomy. The intervention groups received 1.0 mL of 75% hypertonic glucose (Group II; n = 20) or 1.0 mL of 5-Fluorouracil (Group III; n = 20) at the surgical site before suturing. The assessment of the presence of seroma was performed in all animals at 24, 48, and 72 h and on the 7th and 12th postoperative day. After the 12th day, a tissue sample was taken from the surgical site and sent for histological analysis. The occurrence of seroma was assessed using GEE. A significance level of 5% was adopted.

Results

Differences in seroma formation over time were observed for both Control Group I (p=0.041) and Intervention Group II (p<0.001). In Intervention Group III, there was no difference in the percentage and volume of seroma across the assessment points (p=0.627). When both the Control and Intervention Group II were compared to Intervention Group III, we found a reduction in seroma formation in the last group. The reduction in the inflammatory process was more regular to Intervention Group III.

Conclusion

In this animal model, 5-Fluorouracil was more effective in preventing seroma formation than 75% Hypertonic Glucose.

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Authors and Affiliations

  1. Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Rua Botucatu, 740-2° andar-Vila Clementino, CEP:04023-062, Sao Paulo, Sao Paulo, Brasil

    Sidney Bandeira Cartaxo, Luiz Antonio Rosseto, Elvio Bueno Garcia, Rodrigo Cartaxo Bandeira de Melo, Lydia Masako Ferreira & Fabio Xerfan Nahas

  2. Universidade Brasil, Sao Paulo, Sao Paulo, Brasil

    Lazaro Pinto Medeiros Neto

  3. SBCP, Sao Paulo, Sao Paulo, Brasil

    Paulo Guimaraes de Castro Junior

  4. FMABC, Santo André, Sao Paulo, Brasil

    Ricardo Basso

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  1. Sidney Bandeira Cartaxo
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Correspondence to Sidney Bandeira Cartaxo.

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Cartaxo, S.B., Rosseto, L.A., Garcia, E.B. et al. Effectiveness of 5-Fluorouracil and Hypertonic Glucose in the Prevention of Post-mastectomy Seroma in an Experimental Animal Model. Aesth Plast Surg 48, 510–518 (2024). https://doi.org/10.1007/s00266-023-03549-y

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