Abstract
Objective
To investigate whether hyperbaric oxygen therapy can improve the survival rate of fat transplantation and analyze the possible mechanisms.
Methods
Ninety SD rats were randomly divided into 3 groups. All the rats were cut into pieces with about 5 mL of fat from the abdominal cavity, rinsed with normal saline for 3 times, and cleaned with cotton pad adsorption method. Then, 3 ml was removed, divided into 3 parts, and injected into three adjacent but not touching parts of the back. Group A received 1h/d hyperbaric oxygen therapy, group B received 2 h/d hyperbaric oxygen therapy, and group C received no hyperbaric oxygen therapy. The hyperbaric oxygen therapy lasted for 10 consecutive days. Fat grafts from one site were randomly removed at 2, 4, and 6 weeks after surgery, respectively. ① the survival rate of fat transplantation in three groups was compared. ② observe the pathological section; ③ immunohistochemistry was used to detect and compare the expression of vascular endothelial growth factor.
Results
The survival rate of fat transplantation in group A was the highest. After subcutaneous transplantation of 1 ml of fat and 1 hour/day of continuous hyperbaric oxygen treatment for 10 days, the fat survival rates were 0.796 ± 0.071 ml, 0.644 ± 0.151 ml, and 0.473 ± 0.127 ml at the second, fourth, and sixth weeks, respectively. The survival rate of fat transplantation in group B was the second. After subcutaneous transplantation of 1 ml of fat and 2 hour/day of continuous hyperbaric oxygen treatment for 10 days, the survival rate of fat was 0.624 ± 0.220 ml, 0.494 ± 0.125 ml, and 0.329 ± 0.153 ml at the second, fourth, and sixth weeks, respectively. The survival rate of fat transplantation in group C was the lowest. After subcutaneous transplantation of 1 ml of fat and no hyperbaric oxygen treatment for 10 days, the fat survival rates were 0.461 ± 0.132 ml, 0.290 ± 0.112 ml and 0.169 ± 0.091 ml at the second, fourth, and sixth weeks, respectively. We have made changes in the abstract of the article and marked in red color.
Conclusion
Hyperbaric oxygen therapy is conducive to the survival of transplanted fat. Importantly, a short period of hyperbaric oxygen therapy (1 h/d) can promote the survival of transplanted fat.
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References
Bourne DA, James IB, Wang SS, Marra KG, Rubin JP (2016) The architecture of fat grafting: what lies beneath the surface. Plast Reconstr Surg 137(3):1072–1079. https://doi.org/10.1097/01.prs.0000479992.10986.ad
Efrati S, Ben-Jacob E (2014) Reflections on the neurotherapeutic effects of hyperbaric oxygen. Expert Rev Neurother 14(3):233–236. https://doi.org/10.1586/14737175.2014.884928
Qifeng Y, DongMo L, Lingyu HG (2017) Clinical efficacy of fine fat grafting of the body on facial rejuvenation. Chin J Med Aesth Cosmetol 23(1):4–6. https://doi.org/10.3760/cma.j.issn.1671-0290.2017.01.002
H Lu (2017) Evaluation of the effect of different methods for purifying granular fat and its effect on the outcome after transplantation. Peking Union Medical College
Qinghua L, Shuming P, Ying S, Jian J, Jian S, Guiqin Z, Pengjun X (2012) Experimental study of hyperbaric oxygen therapy on sensorineural hearing loss induced by vertebroarterial type cervical spondylosis. China J Rehabil Med 27(4):330–333
Khouri RK Jr, Khouri RER, Lujan-Hernandez JR et al (2014) Diffusion and perfusion: the keys to fat grafting. Plastic Reconstr Surg Global Open 2(9):e220
Suszynski TM, Sieber DA, Cunningham BL et al (2014) Implications of oxygenation in fat grafting. Plast Reconstr Surg 133(5):731e–733e
Luo SJ, Hao XG, Tang SM, Liang J (2005) The function of basic fibroblastic growth factor on revascularization of pearl fat graft transplantation. Zhonghua Zheng Xing Wai Ke Za Zhi 21(2):128–131
Chung CW, Marra KG, Li H et al (2012) VEGF microsphere technology to enhance vascularization in fat grafting. Ann Plast Surg 69(2):213–219
Azzena B, Mazzoleni F, Abatangelo G et al (2008) Autologous platelet-rich plasma as an adipocyte in vivo delivery system: case report. Aesthetic Plast Surg 32(1):155–158
Pallua N, Pilsfort AK, Suschek C et al (2009) Content of the growth factors bFGF, IGF-1, VEGF, and PDGF-BB in freshly harvested lipoaspirate after centrifugation and incubation. Plast Reconstr Surg 123(3):826–833
Suri C, Jones PF, Patan S et al (1996) Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell 87(7):1171–1180. https://doi.org/10.1016/s0092-8674(00)81813-9
Wang YB, Qi KM, Zhao M, Zhuang Q (2004) Leptin as a survival enhanced-factor in grainy fat transplantation. Zhonghua Zheng Xing Wai Ke Za Zhi 20(5):366–368
Lv T, Gu Y, Bi J et al (2019) Fructose 1,6-bisphosphate as a protective agent for experimental fat grafting. Stem Cells Transl Med 8(6):606–616. https://doi.org/10.1002/sctm.18-0212
Thom SR (2011) Hyperbaric oxygen: its mechanisms and efficacy. Plast Reconstr Surg 127(Suppl 1):131S-141S. https://doi.org/10.1097/PRS.0b013e3181fbe2bf
Rhodes CE, Denault D, Varacallo M (2021) Physiology, Oxygen transport. StatPearls Publishing, Treasure Island, FL, November 19
Liang J, Sun X, Yi L, Lv J (2022) Effect of hyperbaric oxygen therapy on weight loss and hyperlipidemia in rats [published correction appears in Biochem Biophys Res Commun. 2022 Apr 16;600:165-166]. Biochem Biophys Res Commun 599:106–112. https://doi.org/10.1016/j.bbrc.2022.02.034
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Rats were maintained under pathogen-free conditions and housed in single cages. All animal experiments were conducted with the approval of the local ethics committee responsible for regulating animal research at The First Affiliated Hospital of Jiamusi University Animal care (permit number: SYXK2021-018) and were maintained strictly according to the guidelines for the care and use of laboratory animals published by the National Institutes of Health (NIH Publication No. 85-23, revised 1996 [https://www.nih.gov/]).
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Liang, J., Sun, X., Yi, L. et al. Effect of Hyperbaric Oxygen Therapy on the Survival Rate of Autologous Fat Transplantation. Aesth Plast Surg 47, 423–429 (2023). https://doi.org/10.1007/s00266-022-03096-y
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DOI: https://doi.org/10.1007/s00266-022-03096-y