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Quercetin May Improve Fat Graft Survival by Promoting Fat Browning Peripherally

  • Original Article
  • Basic Science/Experimental
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Abstract

Background

Adipose browning occurs after white fat transfer. But its location and effects on fat graft survival remains controversial. This study was performed to locate the browning of fat grafts, and to explore the effects of quercetin on fat graft browning and fat graft survival.

Methods

Human fat granules were injected into the subcutaneous layer of 12 nude mice. Control group was injected with fat granules and 10% of normal saline, while quercetin group was injected with fat granules and 10% of quercetin. The graft samples (n = 6 for each group) were obtained in weeks 2, 4, 8 and 12. Weight retention rate of the grafts was calculated. Gene and protein expression of mitochondrial markers (silent information regulator 1, SIRT1; heat shock protein 60, HSP60), browning marker (uncoupling protein 1, UCP1), peroxisome proliferator-activated receptor-γ (PPAR-γ), vascular endothelial growth factor A (VEGF-A) were evaluated. Hematoxylin and eosin staining and anti-UCP1 staining were performed.

Results

Clusters of small multilocular beige adipocytes were observed in the periphery of fat grafts. Compared with control group, quercetin group had a higher weight retention rate, a higher gene/protein expression of SIRT1, HSP60, UCP1, PPAR-γ and VEGF-A, and a higher occurrence of peripheral adipose browning.

Conclusions

Peripherally located adipose browning occurred after white fat transfer. It can be enhanced by the addition of quercetin through promoting mitochondrial function of fat cells, and may be one of the mechanisms that quercetin improves fat graft survival.

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Author notes

  1. Panxi Yu and Zhenyu Yang contributed equally to this article.

Authors and Affiliations

  1. Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, People’s Republic of China

    Panxi Yu, Zhenyu Yang, Haibin Lu, Xiaolei Jin, Xiaonan Yang & Zuoliang Qi

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  1. Panxi Yu
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  2. Zhenyu Yang
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  3. Haibin Lu
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  4. Xiaolei Jin
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  5. Xiaonan Yang
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Correspondence to Xiaonan Yang or Zuoliang Qi.

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The authors declare that they have no conflicts of interest to disclose.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The animal experiment strictly adhered to the ethical guidelines of National Guide for the Care and Use of Laboratory Animals.

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Informed consent was obtained from this patient before using her fat for grafting.

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Yu, P., Yang, Z., Lu, H. et al. Quercetin May Improve Fat Graft Survival by Promoting Fat Browning Peripherally. Aesth Plast Surg 46, 2517–2525 (2022). https://doi.org/10.1007/s00266-022-02857-z

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  • DOI: https://doi.org/10.1007/s00266-022-02857-z

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