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Sub-Flap Use of Nano-Selenium Oxide Solution Enhances Skin Flap Viability in Rats: Study the Novel Role of mTOR and p-mTOR Expression

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  • Basic Science/Experimental
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Abstract

Background

Nano-selenium oxide (NSeO) particles are highly noticeable due to their tissue-protective and antioxidant properties. For this purpose, the effect of NSeO was evaluated on skin flap survival and flap oxidative stress markers in rats. Also, another effect of NSeO was investigated on the expression of mTOR and p-mTOR.

Materials and Methods

Fifty rats were divided into five groups of ten. Skin flap size was 3×8 cm in all groups. Groups were: (1) Sham, (2) Flap Surgery group, (3) Flap Surgery + NSeO, (4) Flap Surgery + Rapamycin (mTOR inhibitor), (5) Flap Surgery + Rapamycin + NSeO. The flap necrosis rate was computed using the paper pattern method on day seven after surgery. After day seven, flap tissues were collected for histological evaluations. Then, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were measured. Furthermore, the expression levels of mTOR and p-mTOR were measured using the Western blot method.

Results

Treatment with NSeO significantly reduced necrosis (P<0.05). It also resulted in a decrease in MDA level (P<0.05). Histologically, NSeO reduced inflammation and increased positive signs of tissue healing (epithelialization, neovascularization, fibroblast migration, and granulation tissue). NSeO increased SOD activity significantly (P<0.05), whereas, using rapamycin reversed these effects. Also, in all groups, mTOR changes were not significant. Additionally, p-mTOR expression was significantly reduced in groups that rapamycin was injected.

Conclusion

NSeO can reduce flap necrosis and enhance tissue healing in rats. So, it can potentially be used clinically to promote tissue repair significantly, and its effects are independent of the mTOR pathway.

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Acknowledgments

The authors are fully grateful to the Iran University of Medical Sciences.

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Authors and Affiliations

  1. Department of Pharmacology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran

    Omid Akhiani & Mohsen Chamanara

  2. Department of Pharmacology and toxicology, School of Pharmacy, Iran university of medical sciences, Tehran, Iran

    Omid Akhiani

  3. Pharmaceutical Sciences Branch, Tehran Islamic Azad University, Tehran, Iran

    Nasim Zangouie

  4. Center for Educational Research in Medical Sciences, University of Medical Sciences, Tehran, Iran

    Reza Laripour

  5. School of Medicine, Aja University of Medical Sciences, Tehran, Iran

    Reza Laripour

  6. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Amir Rashidian

  7. Department of pharmacology and toxicology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran

    Mohsen Ebrahimi

  8. Toxicology Research Center, Aja University of Medical Sciences, 1411718541, Tehran, Iran

    Zahra Hami & Mohsen Chamanara

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Contributions

Study conception and design: OA, ME, NZ, and MC. Acquisition of data: OA, AR, ZH, and RL. Analysis and Interpretation of data: OA, AR, MC, NZ, and RL. Drafting of Manuscript: OA, MC, NZ, and ME. Critical Revision: All authors

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Correspondence to Mohsen Chamanara.

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Akhiani, O., Zangouie, N., Laripour, R. et al. Sub-Flap Use of Nano-Selenium Oxide Solution Enhances Skin Flap Viability in Rats: Study the Novel Role of mTOR and p-mTOR Expression. Aesth Plast Surg 46, 1452–1462 (2022). https://doi.org/10.1007/s00266-021-02681-x

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  • DOI: https://doi.org/10.1007/s00266-021-02681-x

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