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Using New Autologous Tissue Filler Improved Nasolabial Folds−Single-Armed Pilot Study

  • Original Article
  • Non-Surgical Aesthetic
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Abstract

Objective

To evaluate the clinical efficacy and safety of the new autologous tissue filler derived from autologous skin and hairs for correction of nasolabial folds.

Materials and Methods

The new injectable soft tissue filler is prepared from autologous fibroblasts and keratin gel. A total of 40 patients were enrolled in this single-armed pilot study, all of them received the new filler injection treatment for correction nasolabial folds. Following up with the patients, collection of their clinical features, including photographs, satisfaction, and information on adverse events at the 1st, 3rd, 6th, 12th, and 24th months after injection. The clinical efficacy of each patient was evaluated by masked evaluators and independent expert panels, based on the Wrinkle Severity Rating Scale (WSRS) at different time points after the injection.

Results

Thirty-two patients completed the 24-months follow-up, obtaining at least 93.8% improvement, and 75% of them had significantly improved. Base on the self-satisfaction assessment (SSA) indicated that all of them were satisfied with the results. No adverse effects of filler injection treatment were observed during the follow-up.

Conclusions

The combination of autologous keratin and fibroblasts as a new tissue engineering soft tissue filler has shown safety and long-term efficiency in correcting nasolabial folds, with high satisfaction and desirable result.

Level of Evidence IV

This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

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References

  1. Friedman O (2005) Changes associated with the aging face. Facial Plast Surg North Am 12:371

    Article  Google Scholar 

  2. Eppley B, Dadvand B (2006) Injectable soft-tissue fillers: clinical overview. Plast Reconstr Surg 118:98e

    Article  Google Scholar 

  3. Murray CA, Zloty D, Warshawski L (2005) The evolution of soft tissue fillers in clinical practice. Dermatol Clin 23:343

    Article  CAS  Google Scholar 

  4. Kaufman MR, Bradley JP, Dickinson B et al (2007) Autologous fat transfer national consensus survey: trends in techniques for harvest, preparation, and application, and perception of short- and long-term results. Plast Reconstr Surg 119(1):323–331

    Article  CAS  Google Scholar 

  5. DeLorenzi C (2014) Complications of injectable fillers, Part 2: vascular complications. Aesthet Surg J 34(4):584–600

    Article  Google Scholar 

  6. Narins RS, Brandt F, Leyden J et al (2003) A randomized, double-blind, multicenter. comparison of the efficacy and tolerability of Restylane versus Zyplast for the correction of nasolabial folds. Dermatol Surg 29:588–595

    PubMed  Google Scholar 

  7. Schmidt CFDA (2011) Approves first cell therapy for wrinkle-free visage. Nat Bio technol 29:674–675

    Article  CAS  Google Scholar 

  8. Weiss RA, Weiss MA, Beasley KL et al (2007) Autologous cultured fibroblast injection for facial contour deformities: a prospective, placebo-controlled, Phase III clinical trial. Dermatol Surg 33(3):263–268

    CAS  PubMed  Google Scholar 

  9. Wang S, Taraballi F, Tan LP et al (2012) Human keratin hydro- gels support fibroblast attachment and proliferation in vitro. Cell Tissue Res 347(3):795–802

    Article  CAS  Google Scholar 

  10. Ma J, Zhang J, Ma XJ et al (2011) Evaluation of biocompatibility and biodegradability of a new auto-derived injectable soft tissue filler: a preliminary report. Eur J Plast Surg 34(6):479–486

    Article  Google Scholar 

  11. Wang Y, Wang B, Zhang Q et al (2018) New soft tissue filler derived from autologous keratin and fibroblast for neck wrinkles. J Cosmet Dermatol 17(4):600–605

    Article  Google Scholar 

  12. Xing W, Zhang C, Zhang J et al (2019) Correction of tear trough deformity using autologous fibroblast combined with keratin: new soft tissue filler. Aesth Plast Surg. 43(1):221–227

    Article  Google Scholar 

  13. Chen Q, Yang Y, Zhang J et al (2020) Long-lasting treatment for moderate-severe depressed facial scars: skin and hair derived new autologous tissue filler with subcision. J Dermatolog Treat 6:1–7

    CAS  Google Scholar 

  14. Rudkin G, Miller TA (1999) Aging nasolabial fold and treatment by direct excision. Plast Reconstr Surg 104(5):1502-5; discussion 1506-7

  15. Matton G, Anseeuw A, De Keyser F (1985) The history of injectable biomaterials and the biology of collagen. Aesthetic Plast Surg 9(2):133–140

    Article  CAS  Google Scholar 

  16. Ellingsworth LR, DeLustro F, Brennan JE, Sawamura S, McPherson J (1986) The human immune response to reconstituted bovine collagen. J Immunol 136(3):877–882

    CAS  PubMed  Google Scholar 

  17. Downie J (2009) MZ, Rachel L TW, et al, A double-blind, clinical evaluation of facial augmentation treat-ments: a comparison of PRI 1, PRI 2, Zyplast and Perlane. J Plast Reconstr Aesthet Surg 62:1636–1643

    Article  Google Scholar 

  18. Klein AW (2001) Skin filling: collagen and other injectables of the skin. Dermatol Clin 19:491–508

    Article  CAS  Google Scholar 

  19. Narins RS, Brandt F, Leyden J et al (2003) A random-ized, double-blind, multicenter comparison of the efficacy and tolerability of Restylane versus Zyplast for the correction of nasolabial folds. Dermatol Surg 29:588–595

    PubMed  Google Scholar 

  20. Bukhari SNA, Roswandi NL, Waqas M et al (2018) Hyaluronic acid, a promising skin rejuvenating biomedicine: a review of recent updates and pre-clinical and clinical investigations on cosmetic and nutricosmetic effects. Int J Biol Macromol 120(Pt B):1682–1695

    Article  CAS  Google Scholar 

  21. Raspaldo H, De Boulle K, Levy PM (2010) Longevity of effects of hyaluronic acid plus lidocaine facial filler. J Cosmet Dermatol 9(1):11–15

    Article  Google Scholar 

  22. Raspaldo H (2008) Volumizing effect of a new hyaluronic acid sub-dermal facial filler: a retrospective analysis based on 102 cases. J Cosmet Laser Ther 10(3):134–142

    Article  Google Scholar 

  23. Keller G, Sebastian J, Lacombe U et al (2000) Safety of injectable autologous human fibroblasts. Bull Exp Biol Med 130(8):786–789

    Article  CAS  Google Scholar 

  24. Apel PJ, Garrett JP, Sierpinski P et al (2008) Peripheral nerve regeneration using a keratin-based scaffold: long-term functional and histological outcomes in a mouse model. J Hand Surg 33(9):0–1547

  25. Kurkjian TJ, Kenkel JM, Sykes JM et al (2011) Impact of the current economy on facial aesthetic surgery. Aesthet Surg J 31:770–774

  26. Carniol PJ, Heffelfinger RN, Grunebaum LD (2018) Evaluating new technology. Facial Plast Surg Clin North Am 26(2):253–257

    Article  Google Scholar 

  27. Tachibana A, Furuta Y, Takeshima H et al (2002) Fabrication of wool keratin sponge scaffolds for long-term cell cultivation. J Biotechnol 93:165–170

    Article  CAS  Google Scholar 

  28. Zhou FL (2007) Experimental study of autologous fibroblast and hair keratin as soft tissue in nude mice. Southeast University, Nanjing

    Google Scholar 

  29. Tang DJ, Zhang J, Li DB et al (2008) Experimental study of human keratin as tissue fill in Mini pig. Chin J Aesthet Plast Surg 19(2):144–146

    CAS  Google Scholar 

  30. Weiss R, Weiss M, Beasley K et al (2005) Autologous cultured living fibroblast injection for facial contour deformities: a prospective, placebo-controlled, phase III clinical trial. Retour Au Numéro. 52(3):P206

    Google Scholar 

Download references

Acknowledgements

Funding was provided by National Natural Science Foundation of China (Grant Nos. 81571924, 31671312).

Funding

This article was supported by National Natural Science Foundation of China (Nos. 81571924, 31671312).

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Authors and Affiliations

  1. Affiliated Hospital of North China University of Science and Technology, Tang Shan, People’s Republic of China

    Hanyi Jiang

  2. Department of Medical Technology and Engineering, Zheng Zhou Railway Vocational & Technical College, Zheng Zhou, People’s Republic of China

    Ying Guan

  3. Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zheng Zhou, People’s Republic of China

    Jing Yang

  4. Department of Ear Reconstruction, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Shi Jing Shan District, No. 33 Ba-Da-Chu Road, 100144, Beijing, People’s Republic of China

    Jiao Zhang & Qingguo Zhang

  5. North China University of Science and Technology, Tang Shan, People’s Republic of China

    Zhiwei Chen

Authors
  1. Hanyi Jiang
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  2. Ying Guan
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  3. Jing Yang
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  4. Jiao Zhang
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  5. Qingguo Zhang
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  6. Zhiwei Chen
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Contributions

HJ, YG, and JY participated in the clinical application of the technique, follow-up of the patients, analysis of data for the work, and drafted the article. ZC checked the description and proofread, modified, and embellished the language of the paper. JZ and QZ contributed to the conception and design of the study and revised it critically for important intellectual content. All the authors approved the version to be submitted.

Corresponding authors

Correspondence to Jiao Zhang or Qingguo Zhang.

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Conflict of interest

The authors report no financial and personal relationships with other people or organizations that could inappropriately influence the work.

Human and Animal Rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

All of participants were signed written consent forms and known detailed information about the available nasolabial fold treatment. All operations involving human participants are approved by institutional ethics committees and conform to the ethical standards of institutions and national research committees and the ethical standards of the World Medical Association Declaration of Helsinki (June 1964).

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Cite this article

Jiang, H., Guan, Y., Yang, J. et al. Using New Autologous Tissue Filler Improved Nasolabial Folds−Single-Armed Pilot Study. Aesth Plast Surg 45, 2920–2927 (2021). https://doi.org/10.1007/s00266-021-02608-6

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  • DOI: https://doi.org/10.1007/s00266-021-02608-6

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