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Deferoxamine Protects Stromal/Stem Cells of “Lull pgm System”-Processed Lipoaspirates Against Damages Induced by Mitochondrial Respiration Inhibition

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  • Basic Science/Experimental
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Abstract

Background

The ischemic environment of the receiving area compromises the outcome of autologous fat grafts. The aim of this study was to isolate and expand the stromal vascular fraction from patient lipoaspirates and investigate the gain in cell viability exerted by some protective agents against the blockage of mitochondrial respiration.

Methods

The aspirates were (1) washed, using the “Lull pgm system,” (2) centrifuged and (3) decanted. The corresponding stromal vascular fractions were isolated, and after cell adherence selection, the stromal/stem cell subpopulations were exposed to Antimycin A for 1 h. Then, the protection induced by cell pretreatment with deferoxamine, diazoxide and IGF-1 was evaluated.

Results

The residual cell viability of the “Lull pgm system”-washed samples was greater than that of the centrifuged samples (p < 0.05), and this advantage was maintained during the following 12 days of culture. The administration of 400 μM deferoxamine before Antimycin A treatment increased the number of viable cells from 56.5 to 80.8% (p < 0.05). On the contrary, the pretreatment with 250 μM diazoxide or 0.1 μg/ml IGF-1 did not exert any significant pro-survival action. Echinomycin abolished the positive effect of deferoxamine, suggesting that its protection involved HIF-1α.

Conclusions

Adipose-derived stromal–stem cells survive the inhibition of mitochondrial respiration better if the lipoaspirate is washed using the “Lull pgm system” rather than centrifuged. Moreover, a significant contribution to cell survival can be obtained by preconditioning stromal–stem cells with deferoxamine. In a clinical perspective, this drug could be safely administered before surgery to patients undergoing autologous fat transfer.

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Acknowledgements

Funding for this research was obtained from RFO (Ricerca Fondamentale Orientata) of the Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna.

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Author notes

  1. Paolo G. Morselli and Gioia Sorbi have equally contributed to this work.

Authors and Affiliations

  1. Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Bologna, Italy

    Paolo G. Morselli, Gioia Sorbi & Carlotta Feliziani

  2. Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Via Irnerio, 48, 40126, Bologna, Italy

    Claudio Muscari

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  1. Paolo G. Morselli
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Correspondence to Claudio Muscari.

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Morselli, P.G., Sorbi, G., Feliziani, C. et al. Deferoxamine Protects Stromal/Stem Cells of “Lull pgm System”-Processed Lipoaspirates Against Damages Induced by Mitochondrial Respiration Inhibition. Aesth Plast Surg 44, 168–176 (2020). https://doi.org/10.1007/s00266-019-01544-w

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