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Botulinum Toxin Type A: Assessing The Effects on The Brain Stem

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Abstract

Background

In this study, our aim is to investigate the possible effects of Botulinum toxin type A administrations in the early and late periods on the brain stem.

Methods

Eighteen white New Zealand rabbits were used in this study with the subjects being divided into three groups. Group I received 0.05 mL sterile saline to the left anterior auricular muscles. Group II and III were injected with Botulinum toxin type A (Botox, Allergan) to the left anterior auricular muscles. Group II was sacrificed 5 days after application and Group III was sacrificed 12 weeks after application; brain stem tissues were then taken. The samples were examined with Caspase 3, 8, and 9 immunohistochemical stainings.

Results

In the control group with Caspase-3 immune staining, moderate-to-strong immune reactivity was seen in a small number of neurons. In the Caspase-8 and 9 immune stainings, the immune reactive neurons were seen in greater numbers when compared with the Caspase-3 immune reactive neurons. In the early and late period, groups with Caspase-8 and 9 immune stainings, the immune reactive neurons were seen in greater numbers and in the wider area when compared with the Caspase-3 immune reactive neurons. No significant differences were recognized in the Caspase immune stainings between the early and late period groups. The results were statistically supported.

Conclusion

It was concluded that Botulinum toxin type A application did not trigger apoptosis in stem cell tissues.

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Acknowledgements

We would like to thank Dr. Bülent Çelik for the statistical analysis for the study. Mr. Edward McQuaid English Lecturer at Anadolu University (Editing).

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Authors and Affiliations

  1. Department of Histology and Embryology, Gazi University Faculty of Medicine, Building the Academic Deanship, 4th Floor. Besevler, Ankara, Turkey

    Cemile Merve Seymen & Cigdem Elmas

  2. Department of Plastic and Reconstructive Surgery, Gazi University Faculty of Medicine, Ankara, Turkey

    Serhan Tuncer & M. Suhan Ayhan

Authors
  1. Cemile Merve Seymen
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  2. Serhan Tuncer
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  3. M. Suhan Ayhan
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  4. Cigdem Elmas
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Correspondence to Cemile Merve Seymen.

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Seymen, C.M., Tuncer, S., Suhan Ayhan, M. et al. Botulinum Toxin Type A: Assessing The Effects on The Brain Stem. Aesth Plast Surg 42, 538–545 (2018). https://doi.org/10.1007/s00266-018-1092-3

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  • DOI: https://doi.org/10.1007/s00266-018-1092-3

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