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The Fetal Porcine Aorta and Mesenteric Acellular Matrix as Small-caliber Tissue Engineering Vessels and Microvasculature Scaffold

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Abstract

Background

The extracellular matrix (ECM) is characterized by not only well-preserved scaffolds of organs and vascularized tissues, but also by extremely low immunogenicity during allo- or xeno-implantation. This study aimed to establish a model of a composite microvasculature network scaffold with a small-caliber-dominant vascular pedicle by decellularizing fetal porcine aorta and the conterminous mesentery.

Methods

The aorta and the conterminous mesenteric vascular system originating from the inferior mesenteric artery were harvested from fetal pigs at late gestation. All of the cellular components were removed by sequential treatment with Triton X-100 and sodium dodecyl sulfate. After the degree of decellularization was assessed, the fetal porcine aorta and mesenteric acellular matrix (FPAMAM) were transplanted into dogs.

Results

Gross and histologic examination demonstrated the removal of cellular constituents with preservation of ECM architecture, including macrochannels and microchannels. The residual DNA content in the FPAMAM was less than 2 %. The aorta and microchannels were perfused well, and the fetal porcine aorta had good patency for more than 3 months.

Conclusions

The integrity of the FPAMAM provided a scaffold for the reconstruction of a rich vascular network with numerous segmentally radiating branches. Decellularized fetal porcine vascular tissue might be a potential alternative for xenogeneic transplantation based on its optimized properties and low immunogenicity.

Level of Evidence II

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Acknowledgments

This work was supported by the National Science Foundation of China (Grant No. 81171825).

Disclosures

The authors have no conflicts of interest to disclose.

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Authors and Affiliations

  1. Division of Plastic Surgery, The 2nd Hospital of Harbin Medical University, Harbin, Heilongjiang, People’s Republic of China

    Qingchun Li, Chushan Huang, Zelin Xu, Guofeng Liu, Ying Liu, Zhibo Xiao, Chunlei Nie, Binyou Zheng & Daping Yang

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  1. Qingchun Li
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  2. Chushan Huang
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  3. Zelin Xu
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  4. Guofeng Liu
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Correspondence to Binyou Zheng or Daping Yang.

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Li, Q., Huang, C., Xu, Z. et al. The Fetal Porcine Aorta and Mesenteric Acellular Matrix as Small-caliber Tissue Engineering Vessels and Microvasculature Scaffold. Aesth Plast Surg 37, 822–832 (2013). https://doi.org/10.1007/s00266-013-0173-6

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  • DOI: https://doi.org/10.1007/s00266-013-0173-6

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