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Electroacupuncture Attenuates Oxidative Stress in Random Skin Flaps in Rats

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  • Experimental/Special Topics
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Abstract

Background

Skin flaps may be damaged by free radicals produced during blood reflow and oxygen return to ischemic tissues. Considering that published studies have demonstrated the effects of manual acupuncture (MA) and electroacupuncture (EA) in attenuating the oxidative stress in different tissues and organs, this study aimed to investigate the possible protective effects of MA and EA in randomized skin flaps in rats.

Methods

Thirty-two rats were subjected to dorsal skin flap (8 × 2.5 cm) construction and randomly divided into four groups of eight rats each: G1, surgical trauma; G2, MA; G3, EA 3 Hz; and G4, EA 100 Hz. All rats were anesthetized with ketamine (90 mg/kg) + xylazine (10 mg/kg) intraperitoneally on days 1, 3, and 7. MA (G2) and EA (G3/G4) were applied on days 3 and 7 of the experiment. G3 and G4 rats were treated with EA 3 and 100 Hz, respectively. Needles were inserted in GV-14 (Dazhui), GV-2 (Yaoshu), and L-13 (Zhangmen) acupoints and retained for 30 min. Blood and skin samples were collected at the end of the last procedure. The ANOVA/Dunnett test was used for group comparison. Values of p < 0.05 were considered significant.

Results

Skin myeloperoxidase (MPO) activity decreased significantly in G2, G3, and G4 rats. Plasma and skin levels of reduced glutathione (GSH) increased significantly in G3 and G4 rats (p < 0.001) compared with G1 control. Malondialdehyde (MDA) plasma and skin levels increased significantly in G4 compared with G1 rats.

Conclusions

MA/EA decreased MPO activity in the graft, consistent with attenuated inflammation, and increased plasma and tissue GSH, indicative of reduced systemic and local oxidative stress. EA at 100 Hz enhances lipid peroxidation in plasma and skin of rats subjected to surgical stress.

Level of Evidence II

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References

  1. Kerrigan CL (1983) Skin flap failure: pathophysiology. Plast Reconstr Surg 72:766–777

    Article  PubMed  CAS  Google Scholar 

  2. McCord JM (1987) Oxygen derived radicals: a link between reperfusion injury and inflammation. Fed Proc 46:1206–2402

    Google Scholar 

  3. Cino M, Del Maestro RF (1989) Generation of hydrogen peroxide by brain mitochondria: the effect of reoxygenation following postdecapitative ischemia. Arch Biochem Biophys 269:623–638

    Article  PubMed  CAS  Google Scholar 

  4. Angel MF, Narayanan K, Swartz WM, Ramasastry SS, Basford RE, Kuhns DB, Futrell JW (1986) The etiologic role of free radicals in hematoma-induced flap necrosis. Plast Reconstr Surg 77:795–803

    Article  PubMed  CAS  Google Scholar 

  5. Floyd RA (1983) Direct demonstration that ferrous ion complexes of di- and triphosphate nucleotides catalyze hydroxyl free radical formation from hydrogen peroxide. Arch Biochem Biophys 225:263–270

    Article  PubMed  CAS  Google Scholar 

  6. Dröge W (2002) Free radicals in the physiological control of cell function. Physiol Rev 82:47–97

    PubMed  Google Scholar 

  7. Inauen W, Granger DN, Meininger CJ, Schelling ME, Granger HJ, Kvietys PR (1990) An in vitro model of ischemia/reperfusion induced microvascular injury. Am J Physiol 259:G134–G139

    PubMed  CAS  Google Scholar 

  8. Leite MT, Gomes HC, Percário S, Russo CR, Ferreira LM (2007) Dimethyl sulfoxide as a block to the deleterious effect of nicotine in a random skin flap in the rat. Plast Reconstr Surg 120:1819–1822

    Article  PubMed  CAS  Google Scholar 

  9. Ueda K, Nozawa M, Miyasaka M, Akamatsu J, Tajima S (1998) Sulfatide protects rat skin flaps against ischemia-reperfusion injury. J Surg Res 80:200–204

    Article  PubMed  CAS  Google Scholar 

  10. Gideroglu K, Yilmaz F, Aksoy F, Bugdayci G, Saglam I, Fahri Yimaz F (2009) Montelukast protects axial pattern rat skin flaps against ischemia/reperfusion injury. J Surg Res 157:181–186

    Article  PubMed  CAS  Google Scholar 

  11. Chakrabarti AK, Chatterjee K, Ghosh JJ, Ganguly A (1983) Electroacupuncture and its effect on rat hepatic functions. Acupunct Electrother Res 8:111–126

    PubMed  CAS  Google Scholar 

  12. Wu Y, Sun Z, Li Z, Zhao Y, Sun S (2002) Effect of acupuncture on free radicals in rats with early experimental spinal cord injury. J Tradit Chin Med 22:51–54

    PubMed  Google Scholar 

  13. Wang XR, Xiao J, Sun DJ (2003) Myocardial protective effects of electroacupuncture and hypothermia on porcine heart after ischemia/reperfusion. Acupunct Electrother Res 28:193–200

    PubMed  Google Scholar 

  14. Siu FK, Lo SC, Leung MC (2004) Effectiveness of multiple pre-ischemia electro-acupuncture on attenuating lipid peroxidation induced by cerebral ischemia in adult rats. Life Sci 75:1323–1332

    Article  PubMed  CAS  Google Scholar 

  15. Dai M, Ma XP, Shi Z, Wu HG, Zhao TP, Jiang GN (2008) Effect of acupuncture on serum SOD and MDA of rats in menopause. J Acupunct Tuina Sci 6:79–82

    Article  Google Scholar 

  16. Uema D, Orlandi D, Freitas RR, Rodgério T, Yamamura Y, Tabosa AF (2008) Effect of eletroacupuncture on DU-14 (Dazhui), DU-2 (Yaoshu), and Liv-13 (Zhangmen) on the survival of Wistar rats’ dorsal skin flaps. J Burn Care Res 29:353–357

    Article  PubMed  Google Scholar 

  17. Jansen G, Lundeberg T, Samuelson UE, Thomas M (1989) Increased survival of ischaemic musculocutaneous flaps in rats after acupuncture. Acta Physiol Scand 135:555–558

    Article  PubMed  CAS  Google Scholar 

  18. Niina Y, Ikeda K, Iwa M, Sakita M (1997) Effects of electroacupuncture and transcutaneous electrical nerve stimulation on survival of musculocutaneous flap in rats. Am J Chin Med 25:273–280

    Article  PubMed  CAS  Google Scholar 

  19. McFarlane RM, DeYoung G, Henry RA (1965) The design of a pedicle flap in the rat to study necrosis and its prevention. Plast Reconstr Surg 35:177–182

    Article  PubMed  CAS  Google Scholar 

  20. Yin CS, Jeong HS, Park HJ, Baik Y, Yoon MH, Choi CB, Koh HG (2008) A proposed transpositional acupoint system in a mouse and rat model. Res Vet Sci 84:159–165

    Article  PubMed  Google Scholar 

  21. World Health Organization (WHO) (1993) Standard acupuncture nomenclature: a brief explanation of 361 classical acupuncture points and their multilingual comparative list, 2nd edn. WHO Regional Office for the Western Pacific, Manila, Philippines

    Google Scholar 

  22. Uchiyama M, Mihara M (1978) Determination of malondialdehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:271–278

    Article  PubMed  CAS  Google Scholar 

  23. Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205

    Article  PubMed  CAS  Google Scholar 

  24. Sagi A, Ferder M, Levens D, Strauch B (1986) Improved survival of island flaps after prolonged ischemia by perfusion with superoxide dismutase. Plast Reconstr Surg 77:639–644

    Article  PubMed  CAS  Google Scholar 

  25. Lundeberg T (1993) Peripheral effects of sensory nerve stimulation (acupuncture) in inflammation and ischemia. Scand J Rehabil Med Suppl 29:61–86

    PubMed  CAS  Google Scholar 

  26. Liao YY, Seto K, Saito H, Fujita M, Kawakami M (1979) Effect of acupuncture on adrenocortical hormone production. 1. Variation in the ability for adrenocortical hormone production in relation to the duration of acupuncture stimulation. Am J Chin Med 7:362–371

    Article  PubMed  CAS  Google Scholar 

  27. Liao YY, Seto K, Saitoh H, Kawakami M (1981) Effect of acupuncture on adrenocortical hormone production in rabbits with a central lesion. Am J Chin Med 9:61–73

    Article  PubMed  CAS  Google Scholar 

  28. Moon PD, Jeong HJ, Kim SJ, An HJ, Lee HJ, Yang WM, Park SK, Hong SH, Kim HM, Um JY (2007) Use of electroacupuncture at ST36 to inhibit anaphylactic and inflammatory reaction in mice. Neuroimmunomodulation 14:24–31

    Article  PubMed  CAS  Google Scholar 

  29. Evora PRB, Pearson PJ, Seccombe JF, Schaff HV (1996) Ischemia-reperfusion injury: physiopathological aspects and the importance of endothelial function. Arq Bras Cardiol 66:239–245 [in Portuguese]

    PubMed  CAS  Google Scholar 

  30. Tardini DMS, Yoshida WB, Novelli ELB, Sequeira JL (2003) Evaluation of two brain ischemia and reperfusion experimental models in rats with carotid temporary occlusion associated or not to vertebral occlusion. Acta Cir Bras 18:514–517 [in Portuguese]

    Article  Google Scholar 

  31. Yu YP, Ju WP, Li ZG, Wang DZ, Wang YC, Xie AM (2010) Acupuncture inhibits oxidative stress and rotational behavior in 6-hydroxydopamine lesioned rat. Brain Res 8:58–65

    Article  Google Scholar 

  32. Shi X, Zhang LJ, Bai HY, Bao CM, Hu S, Guan L (2010) Effects of electroacupuncture on hepatic blood flow and lipid peroxidation in septic rats. Zhongguo Zhen Jiu 30:397–400 [in Chinese]

    PubMed  Google Scholar 

  33. Silva AH, Figueiredo LM, Dias PA, Prado Neto AX, Vasconcelos PR, Guimarães SB (2011) Electroacupuncture attenuates liver and kidney oxidative stress in anesthetized rats. Acta Cir Bras 26(Suppl 1):60–65

    Article  PubMed  Google Scholar 

  34. Wang H, Pan Y, Xue B, Wang X, Zhao F, Jia J, Liang X, Wang X (2011) The antioxidative effect of electro-acupuncture in a mouse model of parkinson’s disease. PLoS One 6:e19790

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This study was funded by the National Counsel of Technological and Scientific Development, Brazil—CNPq (LPL). We are in debt to Daniele Feijão de Sousa, graduate nurse technician, for her helpful technical assistance.

Conflict of interest

The authors declare that they have no conflicts of interest to disclose.

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Authors and Affiliations

  1. Department of Surgery, Federal University of Ceará, Rua Professor Costa Mendes, 1608/3° andar, Bloco Didático, Fortaleza, CE, 60430-140, Brazil

    Lorena Picanço Lima, Andrea de Oliveira Albuquerque, Joaquim José de Lima Silva, Paulo Roberto Leitão de Vasconcelos & Sergio Botelho Guimarães

  2. Department of Maternal and Child Health, Federal University of Ceará, Rua Professor Costa Mendes, 1608/3º andar, Bloco Didático, Fortaleza, CE, 60430-140, Brazil

    Francisco das Chagas Medeiros

Authors
  1. Lorena Picanço Lima
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  2. Andrea de Oliveira Albuquerque
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  3. Joaquim José de Lima Silva
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  4. Francisco das Chagas Medeiros
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  5. Paulo Roberto Leitão de Vasconcelos
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  6. Sergio Botelho Guimarães
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Correspondence to Sergio Botelho Guimarães.

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Lima, L.P., de Oliveira Albuquerque, A., de Lima Silva, J.J. et al. Electroacupuncture Attenuates Oxidative Stress in Random Skin Flaps in Rats. Aesth Plast Surg 36, 1230–1235 (2012). https://doi.org/10.1007/s00266-012-9926-x

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  • DOI: https://doi.org/10.1007/s00266-012-9926-x

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