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A method of treatment for nonunion after fractures using mesenchymal stromal cells loaded on collagen microspheres and incorporated into platelet-rich plasma clots

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Abstract

Purpose

There is evidence showing that mesenchymal stromal cells (MSC) may constitute a potential therapeutic strategy to induce bone regeneration. In this work, we investigate the capacity of autologous bone marrow (BM) MSC loaded on collagen microspheres (CM) and included into autologous platelet-rich plasma (PRP) clots (MSC/CM/PRP) to induce bone formation in patients with nonunion lesions.

Methods

MSC were isolated from BM cells of patients with nonunion lesions. Phenotypical (marker expression) and functional studies (osteogenic differentiation) were performed. MSC were seeded on CM and included into autologous PRP clot (MSC/CM/PRP). The capacity of MSC/CM/PRP to induce bone formation was evaluated in three patients diagnosed with nonunion.

Results

MSC loaded on CM/PRP clots maintain their biological functions, in vitro. After three months, post-MSC transplantation, all patients showed evidence of osteogenesis at the site of nonunion. After one year, all patients showed a complete healing of the nonunion.

Conclusions

Our results support the use of autologous MSC transplanted as MSC/CM/PRP for the treatment of nonunion fractures. Future studies incorporating a larger number of patients may confirm the results obtained in this work.

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Acknowledgments

This work was supported by funds from FONACIT (Fondo Nacional de Ciencia, Tecnología e Investigación), Project No. G-2005000405, and LOCTI Project No. 2011000906

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Authors and Affiliations

  1. Unidad de Terapia Celular - Laboratorio de Patología Celular y Molecular, Instituto Venezolano de Investigaciones Científicas (IVIC), Apartado 21827, Caracas, 1020-A, Venezuela

    Olga Wittig, Egidio Romano, Dylana Diaz-Solano, Maria Elena Marquez & Jose E. Cardier

  2. Servicio de Traumatología, Hospital Universitario de Caracas, Caracas, 1080, Venezuela

    Cesar González, Pedro Tovar & Rodolfo Aoun

Authors
  1. Olga Wittig
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  2. Egidio Romano
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  3. Cesar González
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  4. Dylana Diaz-Solano
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  5. Maria Elena Marquez
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  6. Pedro Tovar
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  7. Rodolfo Aoun
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  8. Jose E. Cardier
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Corresponding authors

Correspondence to Egidio Romano or Jose E. Cardier.

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Authors declare that they do not have any conflict of interest to disclose.

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Wittig, O., Romano, E., González, C. et al. A method of treatment for nonunion after fractures using mesenchymal stromal cells loaded on collagen microspheres and incorporated into platelet-rich plasma clots. International Orthopaedics (SICOT) 40, 1033–1038 (2016). https://doi.org/10.1007/s00264-016-3130-6

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  • DOI: https://doi.org/10.1007/s00264-016-3130-6

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