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Collagen type I coating stimulates bone regeneration and osteointegration of titanium implants in the osteopenic rat

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Abstract

Purpose

To investigate the effects of titanium implants functionalised with collagen type I (TiColl) on bone regeneration and osteointegration in a healthy and osteopenic rat animal model.

Method

TiColl screws were implanted into the femoral condyles of healthy and osteopenic rats and compared with acid-etched titanium (Ti) screws. The osteointegration process was evaluated by a complementary approach combining microtomographic, histological, histomorphometric and biomechanical investigations at four and 12 weeks.

Results

The TiColl screw also ensured a greater mechanical stability; the push-out values for TiColl screws increased from four to 12 weeks (+28 %). The energy necessary to detach the bone from the screw was significantly higher for TiColl-functionalised screws in comparison to Ti screws (+23 %) at 12 weeks. Histomorphometric investigation revealed that total bone-to-implant contact was higher in TiColl screws in comparison to Ti screws (P < 0.05) and at epiphyseal level, increased bone-to-implant contact was found with TiColl screws in comparison to Ti screws (P < 0.05) in an ovariectomy (OVX) condition. A significant increase in the measured total bone ingrowth from four to 12 weeks was detected for both materials, but more significant for the TiColl material (P < 0.0005). Finally, bone ingrowth in the TiColl group was significantly higher (P < 0.005) in comparison to that of Ti screws in the SHAM condition at metaphyseal level at 12 weeks.

Conclusion

The present results showed that TiColl is effective in promoting implant osteointegration even in compromised bone.

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Acknowledgments

The authors are grateful to the project “PORFESR 2007–2013 ASSE 1 ATTIVITA’ I.1.1 CREAZIONE DI TECNOPOLI PER LA RICERCA INDUSTRIALE” Piattaforma Tecnologica: Scienza della vita—Department “Rizzoli RIT-RESEARCH INNOVATION & TECNOLOGY”. This paper was partially supported by Rizzoli Orthopaedic Institute, “5 PER MILLE Project” (2012–1800 PRECL: “La rigenerazione dei tessuti dell’apparato musculoscheletrico: studi preclinici su sorgente cellulare, scaffolds e fattori legati al paziente) and by Regione Piemonte, mediante la Legge Regionale 04/06 “Sistema regionale della ricerca e innovazione”. PSO ASSE II—Competitività MISURA II.1 BANDO PER L’ACCESSO AGLI INNOVATION VOUCHER

Conflict of interest

M. Morra, C. Cassinelli and D. Bollati are employees of Nobil Bio Ricerche SRL, Portacomaro d’Asti (AT), and they declare the existence of a potential conflict of interest. The others authors declare no conflicts of interest with the materials used in the present evaluation.

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Authors and Affiliations

  1. Laboratory of Biocompatibility, Technological Innovations and Advanced Therapies, RIT Department, Rizzoli Orthopaedic Institute, via di Barbiano 1/10, 40136, Bologna, Italy

    Maria Sartori, Annapaola Parrilli & Milena Fini

  2. Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopaedic Institute, via di Barbiano 1/10, 40136, Bologna, Italy

    Gianluca Giavaresi, Andrea Ferrari, Nicolò Nicoli Aldini & Milena Fini

  3. Department of Medical and Surgical Sciences (DIMEC), University of Bologna, via Massarenti 9, 40138, Bologna, Italy

    Andrea Ferrari

  4. Nobil Bio Ricerche SRL, Via Valcastellana 26, 14037, Portacomaro d’Asti, AT, Italy

    Marco Morra, Clara Cassinelli & Daniele Bollati

Authors
  1. Maria Sartori
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  2. Gianluca Giavaresi
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  3. Annapaola Parrilli
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  4. Andrea Ferrari
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  5. Nicolò Nicoli Aldini
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  6. Marco Morra
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  7. Clara Cassinelli
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  8. Daniele Bollati
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  9. Milena Fini
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Correspondence to Maria Sartori.

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Sartori, M., Giavaresi, G., Parrilli, A. et al. Collagen type I coating stimulates bone regeneration and osteointegration of titanium implants in the osteopenic rat. International Orthopaedics (SICOT) 39, 2041–2052 (2015). https://doi.org/10.1007/s00264-015-2926-0

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  • DOI: https://doi.org/10.1007/s00264-015-2926-0

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