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Establishment of a new methicillin resistant staphylococcus aureus animal model of osteomyelitis

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Abstract

Purpose

The increase in methicillin-resistant Staphylococcus aureus (MRSA) infections is currently a major health care problem. Vancomycin is still often the first-line anti-microbiological agent for treating such infections; however, a recent decline in efficacy of vancomycin in MRSA infections has raised concerns and accelerated the search for new antibiotics. The aim of this study was to establish a MRSA peri-implant osteomyelitis animal model for future testing of new anti-microbiological agents under typical MRSA infection conditions.

Methods

Eighteen randomised NZW-rabbits underwent a standardised surgical procedure with the insertion of a femoral bone implant. Animals were then divided into group 1 (MRSA inoculation, no antibiotics; M/N), group 2 (MRSA inoculation, Vancomyin; M/V), and group 3 (no MRSA inoculation, no antibiotics; N/N). The primary study outcome parameters were animal leucocyte count, animal weight, and animal body temperature at one, seven, and 42 days after surgery. Additionally, a histo-morphometrical score was established and adjusted to a modified histological Smeltzer score.

Results

Macroscopic and histo-morphometrical findings showed a peri-implant osteomyelitis in group 1 with both increased acute and chronic infection parameters in M/N, as compared to M/V and N/N, indicating that vancomycin treatment prevented typical morphological changes of MRSA peri-implant osteomyelitis. Similarly, there was a reduction in animal weight and increase in leucocyte count and body temperature in group 1 (each p < 0.005). Vancomycin treatment again resulted in significantly reduced leucocyte count and body temperature, and increased animal body weight.

Conclusions

Here we have established a peri-implant MRSA osteomyelitis model that successfully combined clinical and laboratory outcome parameters of infection with histo-morphometrical results; this model appears to be valuable for future experimental use and therapeutic monitoring of new anti-microbiological MRSA drugs.

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Authors and Affiliations

  1. Department of Orthopedics, Trauma Surgery and Paraplegiology, Heidelberg University Hospital, Heidelberg, Germany

    Lars Helbig, Helga Lorenz, Cornelia Putz, Arash Moghaddam & Gerhard Schmidmaier

  2. Orthopaedic Centre Hochfranken, Hof, Germany

    Hans Georg Simank

  3. Department of Trauma and Orthopaedics, BG Trauma Centre Ludwigshafen, Ludwigshafen, Germany

    Christoph Wölfl & Thorsten Guehring

  4. Department for Septic Surgery, BG Trauma Centre Ludwigshafen, Ludwigshafen, Germany

    Arnold J. Suda

Authors
  1. Lars Helbig
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  2. Hans Georg Simank
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  3. Helga Lorenz
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  4. Cornelia Putz
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  5. Christoph Wölfl
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  6. Arnold J. Suda
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  9. Thorsten Guehring
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Corresponding author

Correspondence to Arnold J. Suda.

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Helbig, L., Simank, H.G., Lorenz, H. et al. Establishment of a new methicillin resistant staphylococcus aureus animal model of osteomyelitis. International Orthopaedics (SICOT) 38, 891–897 (2014). https://doi.org/10.1007/s00264-013-2149-1

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  • DOI: https://doi.org/10.1007/s00264-013-2149-1

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