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In-vitro analysis of the effect of gentamicin and polyhexanide on bone tissue

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Abstract

Purpose

Though anti-infectives have been used for a long time in surgical procedures, the effect on bone tissue has not been determined for most antibiotics and antiseptics.

Methods

In our in vitro study, 4x4x8 mm3 blocks of rabbit cancellous bone tissue were incubated with Ringer’s solution, gentamicin and Lavasorb® each for time intervals of 15 minutes, 30 minutes, one hour, four hours and eight hours. Samples were examined double blinded through optical and electron microscopy.

Results

Tissue degeneration was observed in all samples. It was low in Ringer’s solution. Samples with Lavasorb showed a moderate degeneration after 15 and 30 minutes, which was accelerated after one hour. Gentamicin led to a moderate degeneration of bone tissue after 15 and 30 minutes and to a more accelerated degeneration after one hour. The effect of gentamicin on bone tissue was more pronounced than the effect of Lavasorb.

Conclusions

This investigation showed that local application of Lavasorb or gentamicin on bone tissue should be restricted to 30 minutes, while Lavasorb showed a better tissue tolerability. This finding could have clinical implications for the management of wounds with open osseous tissue and should be further investigated by in vivo studies.

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Authors and Affiliations

  1. Klinik für Orthopädie und Unfallchirurgie, Neckar-Odenwald-Kliniken GmbH, Knopfweg 1, 74821, Mosbach, Germany

    Hans-Jürgen Kock

  2. Chirurgische Abteilung, HELIOS Klinik Bad Berleburg, An der Gontardslust 7, 57319, Bad Berleburg, Alemanya, Germany

    Dirk Ernst

  3. Labor für Experimentelle Unfallchirurgie, Universitätsklinikum Essen, Essen, Germany

    Frank Jethon

  4. Institut für Medizinische Mikrobiologie, Virologie und Hygiene, Abteilung für Medizinische Mikrobiologie und Krankenhaushygiene, Universität Rostock, Rostock, Germany

    Werner Fabry

Authors
  1. Hans-Jürgen Kock
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  2. Dirk Ernst
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  3. Frank Jethon
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  4. Werner Fabry
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Correspondence to Hans-Jürgen Kock.

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Kock, HJ., Ernst, D., Jethon, F. et al. In-vitro analysis of the effect of gentamicin and polyhexanide on bone tissue. International Orthopaedics (SICOT) 37, 761–767 (2013). https://doi.org/10.1007/s00264-013-1786-8

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  • DOI: https://doi.org/10.1007/s00264-013-1786-8

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