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Polyethylene terephthalate (PET) enhances chondrogenic differentiation of ovine meniscocytes in a hyaluronic acid/polycaprolactone scaffold in vitro

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Abstract

Purpose

The purpose of this study was to assess the effect of polyethylene terephthalate (PET) on proliferation, differentiation, and attachment of ovine meniscocytes seeded in a hyaluronic acid/polycaprolactone biomaterial (BF-1)

Methods

BF-1 (30 % hyaluronic acid and 70 % polycaprolactone) cylinders with PET (CO-PET) or without PET, were seeded with 2x106 ovine meniscus cells. The specimens were harvested in triplets at 12 hours, seven, 14, 21 and 28 days. DNA content was measured to test proliferation, histological analysis for cell morphology, and biochemical assessment of glycosaminoglycan content and RT-PCR for type I and II collagen were used to assess differentiation, with immunohistochemistry as post-translational control. Attachment was evaluated by electronic microscopy at 28 days.

Results

DNA content was consistent and equal across groups, suggesting no effect of PET on cell proliferation. However, the BF-1 CO-PET showed a higher percentage of cells with spherical morphology which is typical for a chondrocytic phenotype. This biomaterial with PET also showed a higher type II collagen mRNA expression and an eightfold higher GAG-content than the material without PET. Small amounts of type I collagen mRNA expression were present on both materials at all time points. PCR results were confirmed by immunohistochemistry.

Conclusion

Addition of PET to a hyaluronic acid/polycaprolactone biomaterial enhances a cartilaginous phenotype, increased type II collagen mRNA expression and a higher GAG production in ovine mensicocytes.

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Acknowledgments

This research was supported by the European Commission Fifth Framework Program (Project title: Innovative materials and technologies for a bio-engineered meniscus substitute, Project No GRD1-2001-40401, Contract No G5RD-CT-2002-00703). The authors wish to thank Guenther Brand and Ruth Gruebl for their assistance with the preparation of the histological specimens.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Orthopaedics, Medical University of Vienna, Waehringer Gürtel 18–20, 1090, Vienna, Austria

    Ulrich Koller, Reinhard Windhager & Catharina Chiari

  2. Center of Regenerative Medicine, Danube University Krems, Dr.-Karl-Dorrek-Straße 30, 3500, Krems, Austria

    Stefan Nehrer

  3. Sports Medicine Research Laboratory, Department of Orthopedic Surgery, Children’s Hospital Boston, Harvard Medical School, 300 Longwood Ave, Boston, MA, 02115, USA

    Patrick Vavken

  4. Harvard Center for Population and Development Studies, Harvard School of Public Health, Boston, MA, 02115, USA

    Patrick Vavken

  5. Core Unit of Biomedical Research, Medical University of Vienna, Waehringer Gürtel 18–20, 1090, Vienna, Austria

    Barbara Kapeller

Authors
  1. Ulrich Koller
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  2. Stefan Nehrer
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  3. Patrick Vavken
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  4. Barbara Kapeller
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  5. Reinhard Windhager
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  6. Catharina Chiari
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Correspondence to Ulrich Koller.

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Koller, U., Nehrer, S., Vavken, P. et al. Polyethylene terephthalate (PET) enhances chondrogenic differentiation of ovine meniscocytes in a hyaluronic acid/polycaprolactone scaffold in vitro. International Orthopaedics (SICOT) 36, 1953–1960 (2012). https://doi.org/10.1007/s00264-012-1534-5

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  • DOI: https://doi.org/10.1007/s00264-012-1534-5

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