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Impacts of age and gender on bone marrow profiles of BMP7, BMPRs and Stro-1+ cells in patients with total hip replacement

International Orthopaedics volume 36, pages 879–886 (2012)Cite this article

Abstract

Purpose

A successful osseointegration relies on the interplay of implant surface and surrounding bone marrow cells. This study was undertaken to investigate the impact of age and gender on the bone marrow composition.

Methods

Bone marrow aspirates were obtained from the discarded metaphysis region of the femoral head in 24 patients with total hip replacement. Flow cytometry was used to measure the expression of Stro-1+ cells and BMP receptors (BMPRs)-expressing cells. ELISA was used to measure bone marrow aspirate bone morphology protein7 (BMP7) concentration.

Results

Our data demonstrates that there are diverse bone marrow profiles (Stro-1+ cell and BMPRs+ cells). There are no differences of Stro-1+ cells, BMPRs+ cells, and BMP7 concentration between male and female patients. Though there are slight increases in the number of Stro-1+ cells and BMPRs+ cells in younger patients (<70 years old) than those of old patients (≥70 years old), the difference is not statistically significant. However, we found a close association between the Stro-1+ cells, BMPR1a cells and BMP7 concentration. In addition, a correlation exists between the number of Stro-1+ cells and BMIs of these patients.

Conclusion

Our data suggests that the age and gender of THR patients have little impact on their bone marrow osteogenic potential. The significance of the number of the Stro-1+ with BMPRs expression on the implant fixation and osseointegration warrants further investigation.

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Abbreviations

BMSC:

Bone marrow stromal cells

BMP:

Bone morphology protein

BMPR:

BMP receptors

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Acknowledgments

This work is supported by the Shanghai Natural Science Foundation (09410706100) to Dr. Weili Wang and Blue Cross Blue Shield of Michigan Foundation (BCBSM) to Dr. Weiping Ren.

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Affiliations

  1. Department of Orthopaedic, Renji Hospital, Shanghai JiaoTong University School of Medicine, 1630 DongFang Road, Shanghai, 200127, China

    Yi Shen, Weili Wang, Xiaomiao Li & Zude Liu

  2. Providence Hospital/Detroit Medical Center Orthopedic Residency, Detroit, MI, USA

    David C. Markel & Weiping Ren

  3. Department of Biomedical Engineering, Wayne State University, 818 W. Hancock, Detroit, MI, 48201, USA

    Weiping Ren

Authors
  1. Yi Shen
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  2. Weili Wang
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  3. Xiaomiao Li
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  4. Zude Liu
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  6. Weiping Ren
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Correspondence to Zude Liu or Weiping Ren.

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Shen, Y., Wang, W., Li, X. et al. Impacts of age and gender on bone marrow profiles of BMP7, BMPRs and Stro-1+ cells in patients with total hip replacement. International Orthopaedics (SICOT) 36, 879–886 (2012). https://doi.org/10.1007/s00264-011-1370-z

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Keywords

  • Bone Marrow Stromal Cell
  • Bone Marrow Aspirate
  • Bone Morphogenic Protein
  • Human Bone Marrow Stromal Cell
  • Marrow Adipose Tissue