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A new osteonecrosis animal model of the femoral head induced by microwave heating and repaired with tissue engineered bone

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Abstract

The objective of this research was to induce a new animal model of osteonecrosis of the femoral head (ONFH) by microwave heating and then repair with tissue engineered bone. The bilateral femoral heads of 84 rabbits were heated by microwave at various temperatures. Tissue engineered bone was used to repair the osteonecrosis of femoral heads induced by microwave heating. The roentgenographic and histological examinations were used to evaluate the results. The femoral heads heated at 55°C for ten minutes showed low density and cystic changes in X-ray photographs, osteonecrosis and repair occurred simultaneously in histology at four and eight weeks, and 69% femoral heads collapsed at 12 weeks. The ability of tissue engineered bone to repair the osteonecrosis was close to that of cancellous bone autograft. The new animal model of ONFH could be induced by microwave heating, and the tissue engineering technique will provide an effective treatment.

Résumé

L’objectif de cette étude est de constituer un modèle animal d’ostéonécrose de la tête fémorale (ONFH) par micro traumatismes et guérison par greffe. Matériel et méthode: les deux têtes fémorales de 84 lapins ont été soumises à des micro traumatismes à des températures variables. L’os a été utilisé pour la réparation de cette ostéonécrose. Un examen radiologique et histologique ont également été réalisés de façon à évaluer les résultats. Résultats: la tête fémorale traumatisée à 55 iàC pendant 10 minutes montre des modifications de densité osseuse avec apparition de kystes sur les radiographies avec une ostéonécrose et une réparation qui apparaît entre 4 et 8 semaines. 69% des têtes fémorales sont effondrées à 12 semaines. La réparation osseuse a été réalisée par de l’os spongieux. Conclusion: ce nouveau modèle animal permet de réaliser des micro traumatismes et montre que la réparation cellulaire peut être réalisée de façon effective.

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Acknowledgments

This study was supported by grants from NIAMS (no. 5R03AR052479), Aircast Foundation (no. P20RR024484), and Natural Science Foundation of Yunnan Province (nos. 2005C0070M and 2007C0003R).

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Authors and Affiliations

  1. Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical College, Kunming, 650032, People’s Republic of China

    Yanlin Li, Rui Han & Yongnian Wang

  2. Department of Orthopaedics, Brown Alpert Medical School, Providence, RI, 02903, USA

    Yanlin Li & Lei Wei

  3. Department of Orthopaedics, The First People’s Hospital of Kunming City, Kunming, 650011, People’s Republic of China

    Chengkui Geng

Authors
  1. Yanlin Li
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  2. Rui Han
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  3. Chengkui Geng
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  4. Yongnian Wang
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  5. Lei Wei
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Correspondence to Yanlin Li.

Additional information

This study was approved by Kunming Medical College IACUC, and the principles of laboratory animal care were followed during the animal experiments.

The content is solely the responsibility of the authors and does not necessarily represent the official view of the National Institutes of Health.

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Li, Y., Han, R., Geng, C. et al. A new osteonecrosis animal model of the femoral head induced by microwave heating and repaired with tissue engineered bone. International Orthopaedics (SICOT) 33, 573–580 (2009). https://doi.org/10.1007/s00264-008-0672-2

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  • DOI: https://doi.org/10.1007/s00264-008-0672-2

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