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Correlation between synovial fluid and serum IL-1β levels after ACL surgery–preliminary report


The possibility of controlling the harmful intra-articular influence of elevated interleukin (IL)-1β synovial fluid concentration after anterior cruciate ligament (ACL) surgery could be useful. We investigated the correlation between serum and synovial fluid IL-1β levels following ACL reconstruction. We measured IL-1β concentration periodically in three synovial fluid and four serum samples in each of 20 patients receiving either autologous conditioned serum (ACS) containing endogenous anti-inflammatory cytokines including IL-1Ra and several growth factors (group A) or placebo (group B). A decrease in IL-1β synovial fluid concentration appeared to be more pronounced in absolute terms in group A. In eight patients serum IL-1β was detected on the 6th postoperative day. In four of them whose synovial fluid levels were over 10 pg/ml on the 6th postoperative day, serum IL-1β was detected on the 10th postoperative day. The results were different in group B. Correlation between serum and synovial fluid IL-1β appearance persists in patients after ACL surgery and ACS application. This study is an example of ACS influence on the ACL healing process controlling the IL-1β levels on the basis of the serum IL-1β detection.


Le contrôle de l’influence intra-articulaire néfaste de la concentration synoviale en l’IL-1β après chirurgie du croisé antérieur. Matériel et méthode: nous avons analysé les corrélations entre le sérum et le liquide synovial avec dosage des taux de l’IL-1β après la reconstruction du croisé antérieur. Nous avons mesuré la concentration de l’IL-1β de façon périodique avec trois prélèvements synoviaux et 4 dosages sériques chez 20 patients recevant un sérum ACS contenant des cytokines anti-inflammatoires incluant IL-1Ra et les différents facteurs de croissance (Groupe A) ou le Placébo (Groupe B). Résultats: la concentration de l’IL-1β dans le liquide synovial diminue de façon importante dans le groupe A. Chez 8 patients l’IL-1β sérique a été détectée au sixième jour post-opératoire. Pour chacun d’entre-eux le taux d’ IL-1β synovial était supérieur à 10 pg/ml, à la sixième heure post-opératoirel’IL-1β sérique a été détecté au 10ème jour post-opératoire. Les résultats sont très différents dans le groupe B. En conclusion, une corrélation persiste entre les taux sériques et les taux synoviaux de l’IL-1β chez les patients après chirurgie du croisé antérieur. Cette étude est un exemple de l’influence de l’ACS dans le processus de cicatrisation en contrôlant les taux de l’IL-1β et en analysant les taux sériques de celui-ci.

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The source of all funding for the study was obtained by the National Health Insurance.

We thank Vera Srsan Zivanovic (Department of General Surgery and Traumatology, General Hospital Varazdin), Damir Poljak (Department of Transfusion Medicine, General Hospital Varazdin) and Renata Jug (Institute of Immunology, Zagreb) for their support.

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Correspondence to Nikica Darabos.

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Darabos, N., Hundric-Haspl, Z., Haspl, M. et al. Correlation between synovial fluid and serum IL-1β levels after ACL surgery–preliminary report. International Orthopaedics (SICOT) 33, 413–418 (2009).

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  • Anterior Cruciate Ligament
  • Synovial Fluid
  • Anterior Cruciate Ligament Reconstruction
  • Tunnel Widening
  • Follow Anterior Cruciate Ligament Reconstruction