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Structural changes in the lengthened rabbit muscle

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Abstract

This study evaluated the histological changes in muscle tissue after limb lengthening in skeletally mature and immature rabbits and assessed the most vulnerable level of striated muscle. Twenty-three male domestic white rabbits, divided into six groups, were operated on and different lengthening protocols were used in the mature and immature rabbits. The histopathological changes were analysed by a semi-quantitative method according to the scoring system of Lee et al. (Acta Orthop Scand 64(6):688–692, 1993). After the evaluation of the five main degenerative parameters (muscle atrophy, muscle nuclei internalisation, degeneration of the muscle fibre, perimysial and endomysial fibrosis, haematomas), it is evident that the adults lengthened at a rate of 1.6 mm/day showed more degenerative changes than those lengthened at 0.8 mm/day. The adult 1.6 mm/day lengthened group presented significantly higher damage in the muscle and lower regenerative signs compared with the young 1.6 mm/day lengthened group, according to the summarised degenerative scores.

Résumé

Objectif: Cette étude a pour but d'évaluer les changements histologiques des muscles après allongement squelettique chez des lapines matures ou immatures. Elle permet d'évaluer quel est le niveau de lésion des muscles striés. Matériel et méthode: 23 lapins mâles domestiques blancs ont été divisés en six groupes et traités avec différents protocoles d'allongements.. Les changements histologiques ont été analysés avec une méthode semi quantitative utilisant le score de LEE et collaborateurs. Résultats et conclusion: l'évaluation des cinq principaux paramètres de dégénérescence (atrophie musculaire, noyau musculaire, internalisation, dégénérescence des fibres musculaires, fibrose du périmysium et de l'endomysium, hématome). Il apparaît évident que l'allongement chez l'adulte à un rythme de 1,6 mm par jour entraîne plus de lésions dégénératives que les sujets allongés au rythme de 0,8 mm par jour. Le groupe de lapins adultes avec allongement de 1,6 mm par jour présente des lésions hautement significatives du muscle, avec des signes de régénération musculaire moins importants que ceux du groupe de lapins plus immatures allongés au même rythme, ceci en utilisant des scores identiques.

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References

  1. Aronson J (1994) Temporal and spatial increases in blood flow during distraction osteogenesis. Clin Orthop Relat Res 301:124–131

    PubMed  Google Scholar 

  2. Caiozzo VJ, Utkan A, Chou R, Khalafi A, Chandra H, Baker M, Rourke B, Adams G, Baldwin K, Green S (2002) Effects of distraction on muscle length: mechanisms involved in sarcomerogenesis. Clin Orthop Relat Res 403S:133–145

    Article  Google Scholar 

  3. Cooper RN, Tajbakhsh S, Mouly V, Cossu G, Buckingham M, Butler-Browne GS (1999) In vivo satellite cell activation via Myf5 and MyoD in regenerating mouse skeletal muscle. J Cell Sci 112:2895–2901

    PubMed  CAS  Google Scholar 

  4. Day CS, Moreland MS, Floyd SS Jr, Huard J (1997) Limb lengthening promotes muscle growth. J Orthop Res 15:227–234

    Article  PubMed  CAS  Google Scholar 

  5. Dubowitz V (1985) Muscle biopsy: a practical approach, 2nd edn. Bailliere Tindall, London

    Google Scholar 

  6. Engel AG, Banker BQ (1986) Myology. McGraw-Hill, New York

    Google Scholar 

  7. Hawke TJ, Garry DJ (2001) Myogenic satellite cells: physiology to molecular biology. J Appl Physiol 91:534–551

    PubMed  CAS  Google Scholar 

  8. Jones DA, Round JM (1990) Skeletal muscle in health and disease. A text book of muscle physiology. Manchester University Press, Manchester

    Google Scholar 

  9. Lee DY, Choi IH, Chung CY, Chung PH, Chi JG, Suh YL (1993) Effect of tibial lengthening on the gastrocnemius muscle. A histopathologic and morphometric study in rabbits. Acta Orthop Scand 64(6):688–692

    Article  PubMed  CAS  Google Scholar 

  10. Leung KS, Cheung WH, Yeung HY, Lee KM, Fung KP (2004) Effect of weightbearing on bone formation during distraction osteogenesis. Clin Orthop Relat Res 419:251–257

    Article  PubMed  Google Scholar 

  11. Lindsey CA, Makarov MR, Shoemaker S, Birch JG, Buschang PH, Cherkashin AM, Welch RD, Samchukov ML (2002) The effect of the amount of limb lengthening on skeletal muscle. Clin Orthop Relat Res 402:278–87

    Article  PubMed  Google Scholar 

  12. Makarov MR, Kochutina LN, Samchukov ML, Birch JG, Welch RD (2001) Effect of rhythm and level of distraction on muscle structure: an animal study. Clin Orthop Relat Res 384:250–264

    Article  PubMed  Google Scholar 

  13. Mastalgia FL, Dawkins RL, Papadimitrou JM (1975) Morphological changes in skeletal muscle after transplantation. A light and electron-microscopic study of the initial phases of degeneration and regeneration. J Neuro Sci 25:227–247

    Article  Google Scholar 

  14. Matano T, Tamai K, Kurokawa T (1994) Adaptation of skeletal muscle in limb lengthening: a light diffraction study on the sarcomere length in situ. J Orthop Res 12:193–196

    Article  PubMed  CAS  Google Scholar 

  15. Meffert RH, Tis JE, Inoue N, McCarthy EF, Brug E, Chao EYS (2000) Primary resective shortening followed by distraction osteogenesis for limb reconstruction: a comparison with simple lengthening. J Orthop Res 18:629–636

    Article  PubMed  CAS  Google Scholar 

  16. Sangkaew C (2005) Distraction osteogenesis for the treatment of post traumatic complications using a conventional external fixator. A novel technique. Injury 36:185–193

    PubMed  Google Scholar 

  17. Schultz E, McCormick KM (1994) Skeletal muscle satellite cells. Rev Physiol Biochem Pharmacol 123:213–257

    Article  PubMed  CAS  Google Scholar 

  18. Schumacher B, Keller J, Hvid I (1994) Distraction effects on muscle. Leg lengthening studied in rabbits. Acta Orthop Scand 65:647–650

    PubMed  CAS  Google Scholar 

  19. Shisha T, Kiss S, Pap K, Simpson H, Szőke G (2006) Relative ability of young and mature muscles to respond to limb lengthening. J Bone Joint Surg Br 88(12):1666–1669

    Article  PubMed  CAS  Google Scholar 

  20. Simpson AH, Williams PE, Kyberd P, Goldspink G, Kenwright J (1995) The response of muscle to leg lengthening. J Bone Joint Surg Br 77:630–636

    PubMed  CAS  Google Scholar 

  21. Tamai K, Kurokawa T, Matsubara I (1989) In situ observation of adjustment of sarcomere length in skeletal muscle under sustained stretch. J Jpn Orthop Assoc 63:1558–1563

    CAS  Google Scholar 

  22. Tatsumi R, Sheehan SM, Iwasaki H, Hattori A, Allen RE (2001) Mechanical stretch induces activation of skeletal muscle satellite cells in vitro. Exp Cell Res 267:107–114

    Article  PubMed  CAS  Google Scholar 

  23. Tsujimura T, Kinoshita M, Abe M (2006) Response of rabbit skeletal muscle to tibial lengthening. J Orthop Sci 11:185–90

    Article  PubMed  Google Scholar 

  24. Saleh M, Hamer AJ (1993) Bifocal limb lengthening: a preliminary report. J Pediatr Orthop B 2:42–48

    Google Scholar 

  25. Williams P, Simpson H, Kyberd P, Kenwright J, Goldspink G (1999) Effect of rate of distraction on loss of range of joint movement, muscle stiffness, and intramuscular connective tissue content during surgical limb-lengthening: a study in the rabbit. Anat Rec 255:78–83

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Orthopaedics, Semmelweis University of Budapest, 27 Karolina Street, Budapest, 1113, Hungary

    Károly Pap, Tamás Shisha, Sándor Kiss & György Szőke

  2. Department of Traumatology and Bone and Joint Reconstructive Surgery, Szentes University Teaching Hospital, Szentes, Hungary

    Sándor Berki

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  1. Károly Pap
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  2. Sándor Berki
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  3. Tamás Shisha
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  4. Sándor Kiss
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  5. György Szőke
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Correspondence to Károly Pap.

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Pap, K., Berki, S., Shisha, T. et al. Structural changes in the lengthened rabbit muscle. International Orthopaedics (SICOT) 33, 561–566 (2009). https://doi.org/10.1007/s00264-008-0514-2

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  • DOI: https://doi.org/10.1007/s00264-008-0514-2

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