International Orthopaedics

, Volume 30, Issue 3, pp 147–152 | Cite as

Demineralized bone matrix and hydroxyapatite/tri-calcium phosphate mixture for bone healing in rats

  • Ali ÖztürkEmail author
  • H. Yetkin
  • L. Memis
  • E. Cila
  • S. Bolukbasi
  • C. Gemalmaz
Original Paper


Purpose: Hydroxyapatite/tri-calcium phosphate (HA/TCP) mixture is an osteoconductive material used as a bone graft substitute, and demineralised bone matrix (DBM) is an osteoinductive material. A combination of DBM and HA/TCP mixture would probably create a composite with both osteoconductive and osteoinductive properties. The purpose of this study was to determine the effect of the combination of DBM and HA/TCP mixture on healing of rat radius segmental defects. Methods: Twenty-four adult male Wistar rats were used. Bilateral radial defects were created in each animal. Radial defects were implanted with DBM, HA/TCP mixture and a combination of both substances. Control defects were left unfilled. Ten weeks after implantation, the animals were sacrificed, and the radii were evaluated by radiograhic and histopathological studies. Results: The use of DBM alone demonstrated improved healing on radiographic and histological studies compared to other groups and the control group. There were no differences between the other two groups and the control group. Conclusion: The DBM group showed the best healing response. Combined use of DBM and HA/TCP mixture did not improve bone healing, and the osteoinductive properties of DBM were inhibited by HA/TCP mixture.


Fracture Healing Segmental Defect Demineralized Bone Matrix Autogenous Bone Bone Graft Substitute 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Le mélange hydroxyapatite/phosphate tri-calcique (HA/TCP) est un matériel d’ostéo conduction utilisé comme greffe ou substitut osseux comme les matrices osseuses déminéralisées (DBM) utilisées en tant que matériel d’ostéo induction. La combinaison de DBM et HA/TCP devrait probablement avoir les deux propriétés d’ostéo induction et d’ostéo conduction. Le propos de cette étude est d’évaluer les effets d’un mélange de DBM et d’HA/TCP sur la consolidation de défauts osseux du radius chez le rat. Vingt quatre rats adultes Wistar ont été utilisés. Chaque animal était préparé avec un defect radial bilatéral. Les radius droits des rats ont été implantés soit avec DBM, soit avec HA/TCP isolée, soit avec une combinaison des deux substances, le radius gauche servant de contrôle. Dix semaines après l’implantation, les animaux ont été sacrifiés et les os ont été évalués sur la plan radiographique et histopathologique. L’utilisation de DBM isolée montre une amélioration de la guérison sur le plan radiographique et histologique. On ne retrouve pas de différence avec les autres groupes. En conclusion, le groupe DBM montre une bonne réponse sur le plan de la consolidation. L’utilisation d’une mixture DBM et HA/TCP n’entraîne pas une amélioration et une guérison, les propriétés d’ostéo induction du DBM étant inhibées par le mélange HA/TCP.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Ali Öztürk
    • 1
    • 3
    Email author
  • H. Yetkin
    • 1
  • L. Memis
    • 2
  • E. Cila
    • 1
  • S. Bolukbasi
    • 1
  • C. Gemalmaz
    • 1
  1. 1.Department of Orthopaedics and TraumatologyGazi University, Faculty of MedicineAnkaraTurkey
  2. 2.Faculty of Medicine, Department of PathologyGazi UniversityAnkaraTurkey
  3. 3.Department of Orthopaedics and TraumatologyGazi University, Faculty of MedicineBahcelievler No:53/11, CorumTurkey

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