Multimer technologies for detection and adoptive transfer of antigen-specific T cells

  • Rosaely Casalegno-Garduño
  • Anita Schmitt
  • Junxia Yao
  • Xinchao Wang
  • Xun Xu
  • Mathias Freund
  • Michael Schmitt
Review

Abstract

Identification and purification of antigen-specific T cells without altering their functional status are of high scientific and clinical interest. Staining with major histocompatibility complex (MHC)-peptide multimers constitutes a very powerful method to study antigen-specific T-cell subpopulations, allowing their direct visualization and quantification. MHC-peptide multimers, such as dimers, tetramers, pentamers, streptamers, dextramers and octamers have been used to evaluate the frequency of CD8+ T cells, specific for tumor/leukemia-associated antigens as well as for viral antigens, e.g., CMVpp65 and EBV-EBNA. Moreover, MHC-peptide multimers have been used for rapid and efficient ex vivo isolation and expansion of T cells. A recent development in the field of MHC-peptide multimers led to the purification of CD8+ T cells specific for leukemia antigens. This might help to select leukemia-specific donor lymphocyte infusions (DLIs), thus allowing dissection of the noxious graft-versus-host disease (GvHD) from beneficial anti-viral and even anti-leukemic effects. This review covers different types of MHC-peptide multimers and their applications, as well as the impact that multimers might have on further development of DLIs.

Keywords

Tetramers Streptamers Flow cytometry Adoptive T-cell transfer 

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Rosaely Casalegno-Garduño
    • 1
  • Anita Schmitt
    • 1
  • Junxia Yao
    • 2
  • Xinchao Wang
    • 1
    • 3
  • Xun Xu
    • 1
    • 4
  • Mathias Freund
    • 1
  • Michael Schmitt
    • 1
  1. 1.Department of Internal Medicine III, Clinical Stem Cell Transplantation and ImmunotherapyUniversity Clinic RostockRostockGermany
  2. 2.Center for Stem Cell Research and Application, Institute of Hematology, Union HospitalHuazhong University of Science and TechnologyWuhanChina
  3. 3.Department of Oncology and HematologyZhongda Hospital, Southeast UniversityNanjingChina
  4. 4.Department of ImmunologyJiangsu UniversityZhenjiangChina

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