Abstract
Purpose
The present study evaluated mRNA expression of interferon-alpha (IFN-α), IFN-α receptor subunits (IFNAR-1 and IFNAR-2) and an IFN-stimulated gene encoding the enzyme 2′,5′-oligoadenylate synthetase (2′5′OAS) in biopsies on patients with varying grades of cervical intraepithelial neoplasia (CIN I, II and III).
Methods
Uterine cervix biopsies were collected from women with CIN I, II and III (n = 28) and controls without CIN lesions or human papilloma virus (HPV) infection (n = 17). The presence of high and low-risk HPV DNA was determined using hybrid capture. The mRNA levels of IFNAR-1, IFNAR-2, IFN-α and 2′5′OAS were determined by RT-PCR with specific primers.
Results
The control group exhibited a greater frequency of IFNAR-1 expression (10/17; 58.3%) than the CIN samples (4/28; 14.2%) (P = 0.0018), while, the expression of IFNAR-2 was also greater in the control samples (11/17; 64.7%) than in the patients with lesions (2/28; 7.1%) (P = 0.0018). Importantly, simultaneous expression of both receptors was observed only in the control group (8/17; 47.0%) (P = 0.0001). Among the CIN samples, there was one case of low expression of mRNA of IFNAR-1 and IFNAR-2. IFN-α was present in 14.2% (4/28) of the CIN samples but was not expressed in the control group. mRNA 2′5′OAS were expressed in 28.5% (8/28) of the CIN samples and 11.7% (2/17) of the control samples (not statistically significant). Fifty percent (14/28) of the CIN samples were positive for HPV DNA.
Conclusions
Cervical biopsy samples from control women or those without neoplasia or HPV infection displayed higher IFN-α receptor expression than those with CIN, while simultaneous expression of both IFN-α receptor subunits was found only in the control group. There was no significant difference in mRNA expression of IFN-α and 2′5′OAS between the control and CIN groups. Then we concluded that the samples obtained from patients with CIN present low levels of the IFN-α receptor mRNA.
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Acknowledgments
The authors thank Research and Projects Financing (FINEP), the Support Research Foundation of State of Minas Gerais (FAPEMIG), and the National Council for Scientific and Technological Development (CNPq) for financial support.
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Tirone, N.R., Peghini, B.C., Barcelos, A.C.M. et al. Local expression of interferon-alpha and interferon receptors in cervical intraepithelial neoplasia. Cancer Immunol Immunother 58, 2003–2010 (2009). https://doi.org/10.1007/s00262-009-0707-6
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DOI: https://doi.org/10.1007/s00262-009-0707-6