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Effect of BCG stimulus on proinflammatory cytokine production in laryngeal cancer

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Abstract

Background

Evaluate the production of TNF and IL-6 in the supernatant of peripheral blood mononuclear cell (PBMC) cultures of patients with supraglottic laryngeal cancer before and after surgical treatment.

Materials and methods

Adherent cell cultures were stimulated with LPS and BCG. Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cytokine concentration was determined by ELISA in supernatants of mononuclear cell cultures.

Results

In non-stimulated cultures, lower TNF cytokine levels were detected during the late postoperative (LP) period compared to control (P = 0.02). LP TNF and IL-6 levels were high in cultures stimulated with LPS compared with the preoperative period (PREOP) (P = 0.007; P = 0.008, respectively). Stimulation with BCG led to increased levels of TNF and IL-6 during the LP period compared to control (P = 0.001; P = 0.04, respectively).

Conclusion

BCG is able to modulate the immune response of patients with advanced supraglottic laryngeal cancer in vitro, increasing the secretion of TNF and IL-6 by macrophages during the postoperative period.

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Authors and Affiliations

  1. Department of Head and Neck Surgery, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil

    Luiz Carlos Conti-Freitas & Rui Celso Martins Mamede

  2. Department of Internal Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil

    Maria Cristina Foss-Freitas & Norma Tiraboschi Foss

Authors
  1. Luiz Carlos Conti-Freitas
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  2. Maria Cristina Foss-Freitas
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  3. Rui Celso Martins Mamede
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  4. Norma Tiraboschi Foss
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Correspondence to Luiz Carlos Conti-Freitas.

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Conti-Freitas, L.C., Foss-Freitas, M.C., Mamede, R.C.M. et al. Effect of BCG stimulus on proinflammatory cytokine production in laryngeal cancer. Cancer Immunol Immunother 58, 25–29 (2009). https://doi.org/10.1007/s00262-008-0520-7

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  • DOI: https://doi.org/10.1007/s00262-008-0520-7

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