Abstract
A panel of cytolytic T lymphocyte (CTL) clones was isolated from metastases and blood samples of a melanoma patient vaccinated with MAGE-3.A1-pulsed autologous dendritic cells. We report here the identification of a new antigen encoded by the MAGE-C2 cancer-germline gene. This antigen is recognized by some of these CTL on HLA-B*4403. The sequence of the peptide is SESIKKKVL. It is processed in various melanoma cell lines expressing MAGE-C2 and HLA-B*4403. Because of the expression pattern of gene MAGE-C2, this new antigen is strictly tumor-specific and could therefore be used for peptide-based antitumoral vaccination.
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Acknowledgments
We thank V. Ha Thi, M. Panagiotakopoulos and M. Swinarska for technical assistance, W. Ma for providing the MAGE-C2 minigenes, and V. Stroobant for peptide synthesis. We thank P. van der Bruggen for critical reading, and N. Krack for editorial assistance. This work was supported by the Belgian Programme on Interuniversity Poles of Attraction initiated by the Belgian State, Prime Minister’s Office, Science Policy Programming, and by a grant of the Fédération Belge contre le Cancer. Javier Carrasco was supported by a grant FNRS-TELEVIE.
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Godelaine, D., Carrasco, J., Brasseur, F. et al. A new tumor-specific antigen encoded by MAGE-C2 and presented to cytolytic T lymphocytes by HLA-B44. Cancer Immunol Immunother 56, 753–759 (2007). https://doi.org/10.1007/s00262-006-0244-5
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DOI: https://doi.org/10.1007/s00262-006-0244-5