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HLA-A0201 positive pancreatic cell lines: new findings and discrepancies

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Abstract

Pancreatic cancer is being pursued as an immunotherapy target using antigen-specific vaccine approaches activating CD8+ CTL and CD4+ T-helper cells. CD8+ CTL exert their anti-tumor effects in an HLA-restricted manner and only tumor cells carrying a matched HLA class I sub-type are targets for antigen-specific CTL. In the process of characterizing CD8+ T cell responses against pancreatic cancer, we screened a number of human pancreatic tumor cell lines for HLA-A0201 positive (HLA-A2+) cell lines to be used in the evaluation of CTL function. This analysis revealed some new findings and discrepancies in the literature on the HLA sub-type of some commonly used pancreatic cell lines. We found that Capan-1 cells, originally reported to be HLA-A0201+, actually only express HLA-A010101 and HLA-A300101 and were targets for HLA-A0201-restricted CTL only after transduction with an HLA-A0201-expressing lentivirus. Panc-1 cells were found to be HLA-A0201 positive, in agreement with published reports, while CF-Pac-1 cells were found to express both HLA-A020101 and HLA-A030101. We also found a normal human pancreatic ductal epithelial cell line, HPDE, to be HLA-A0201 positive. Our findings were verified with two different sequence-based typing methods, antibody staining followed by flow cytometry analysis, and functional analysis using an HLA-A0201-restricted peptide-specific T cell response.

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Authors and Affiliations

  1. Department of Gastrointestinal Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Kuichun Zhu, James L. Abbruzzese & David Z. Chang

  2. Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Kuichun Zhu, Gregory Lizee, Patrick Hwu & Laszlo Radvanyi

  3. HLA Typing Laboratory, University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Pedro Cano & Marcelo Fernando-Vina

  4. Department of Cancer Biology, University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Baoan Ji

  5. University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX, 77030, USA

    Laszlo Radvanyi

Authors
  1. Kuichun Zhu
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  2. Gregory Lizee
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  3. Pedro Cano
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  4. Marcelo Fernando-Vina
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  5. Baoan Ji
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  6. James L. Abbruzzese
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  7. Patrick Hwu
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  8. Laszlo Radvanyi
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  9. David Z. Chang
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Corresponding authors

Correspondence to Laszlo Radvanyi or David Z. Chang.

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Zhu, K., Lizee, G., Cano, P. et al. HLA-A0201 positive pancreatic cell lines: new findings and discrepancies. Cancer Immunol Immunother 56, 719–724 (2007). https://doi.org/10.1007/s00262-006-0217-8

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  • DOI: https://doi.org/10.1007/s00262-006-0217-8

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