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Characterization of glycosylation and adherent properties of melanoma cell lines

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Abstract

The repertoire of oligosaccharide components of cellular glycoproteins significantly contributes to cell adhesion and communication. In tumor cells, alteration in cellular glycosylation may play a key role in giving rise to invasive and metastatic potential. Over 100 melanoma cell lines deposited in the ESTDAB Melanoma Cell Bank (Tubingen, Germany) were studied for the characteristic glycan composition related to tumor progression. Analysis of: (1) cell adhesion to extracellular matrix proteins—fibronectin, laminin, and collagen; (2) the expression of selected glycosyltransferases—α2,3(Galβ1,3)- and α2,3(Galβ1,4)-sialyltransferases, α1,2- and α1,3-fucosyltransferases, and N-acetylglucosaminyltransferase V; (3) characterization of N-glycans was carried out on uveal (4), primary cutaneous (6), and metastatic (96) melanoma cell lines. Results showed that uveal cells did not adhere to any of the substrates and, in general, possessed less glycans containing α-2,6- and α-2,3-linked sialic acid. The average number of polypeptides bearing β-1,6-branched tri- and tetra antennary glycans(characteristic of the metastatic phenotype)were similar in uveal, primary cutaneous, and metastatic melanoma cell lines. Characterization of N-glycans may open a new perspective in the search for specific glycoproteins that could become targets for the therapeutic modulation of melanoma.

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Abbreviations

AAA:

Aleuria aurantia agglutinin

BSA:

Bovine serum albumin

ECM:

Extracellular matrix

DSA:

Datura stramonium agglutinin

GAPDH:

Glyceraldehydes-3-phosphate dehydrogenase

GlcNAc:

N-acetylglucosamine

GnT-V:

N-acetylglucosaminyltransferase V

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Acknowledgements

The studies were carried out and fully financed within the FP5 EU project “European Searchable Tumor Cell Lines Data Bank” – ESTDAB-NAS: QLRI-CT-2001-01325. The authors would like to thank Prof. Graham Pawelec for comments and fruitful discussion.

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Authors and Affiliations

  1. Institute of Medical Biochemistry, Jagiellonian University Medical College, ul. Kopernika 7, 31-034, Kraków, Poland

    Piotr Laidler, Maria Łabędz & Dorota Ciołczyk-Wierzbicka

  2. Institute of Zoology, Glycobiology Laboratory, ul. Ingardena 6, 30-060, Kraków, Poland

    Anna Lityńska, Dorota Hoja-Łukowicz, Małgorzata Przybyło, Ewa Pocheć, Ewa Trębacz & Elżbieta Kremser

Authors
  1. Piotr Laidler
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  2. Anna Lityńska
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  3. Dorota Hoja-Łukowicz
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  5. Małgorzata Przybyło
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  6. Dorota Ciołczyk-Wierzbicka
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  7. Ewa Pocheć
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  8. Ewa Trębacz
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  9. Elżbieta Kremser
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Correspondence to Piotr Laidler.

Additional information

This article is a symposium paper from the conference “Progress in Vaccination against Cancer 2004 (PIVAC 4)”, held in Freudenstadt-Lauterbad, Black Forest, Germany, on 22–25 September 2004

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Laidler, P., Lityńska, A., Hoja-Łukowicz, D. et al. Characterization of glycosylation and adherent properties of melanoma cell lines. Cancer Immunol Immunother 55, 112–118 (2006). https://doi.org/10.1007/s00262-005-0019-4

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  • DOI: https://doi.org/10.1007/s00262-005-0019-4

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