Abstract
Purpose
Although 15O-O2 gas inhalation can provide a reliable and accurate myocardial metabolic rate for oxygen by PET, the spillover from gas volume in the lung distorts the images. Recently, we developed an injectable method in which blood takes up 15O-O2 from an artificial lung, and this made it possible to estimate oxygen metabolism without the inhalation protocol. In the present study, we evaluated the effectiveness of the injectable 15O-O2 system in porcine hearts.
Methods
PET scans were performed after bolus injection and continuous infusion of injectable 15O-O2 via a shunt between the femoral artery and the vein in normal pigs. The injection method was compared to the inhalation method. The oxygen extraction fraction (OEF) in the lateral walls of the heart was calculated by a compartmental model in view of the spillover and partial volume effect.
Results
A significant decrease of lung radioactivity in PET images was observed compared to the continuous inhalation of 15O-O2 gas. Furthermore, the injectable 15O-O2 system provides a measurement of OEF in lateral walls of the heart that is similar to the continuous-inhalation method (0.71 ± 0.036 and 0.72 ± 0.020 for the bolus-injection and continuous-infusion methods, respectively).
Conclusion
These results indicate that injectable 15O-O2 has the potential to evaluate myocardial oxygen metabolism.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ohtake T. The review of myocardial positron emission computed tomography and positron imaging by gamma camera. Kaku Igaku. 1998;35:179–87.
Klein LJ, Visser FC, Knaapen P, Peters JH, Teule GJ, Visser CA, et al. Carbon-11 acetate as a tracer of myocardial oxygen consumption. Eur J Nucl Med. 2001;28:651–68.
Schelbert HR. PET contributions to understanding normal and abnormal cardiac perfusion and metabolism. Ann Biomed Eng. 2000;28:922–9.
Visser FC. Imaging of cardiac metabolism using radiolabelled glucose, fatty acids and acetate. Coron Artery Dis. 2001;12(Suppl 1):S12–8.
Hata T, Nohara R, Fujita M, Hosokawa R, Lee L, Kudo T, et al. Noninvasive assessment of myocardial viability by positron emission tomography with 11C acetate in patients with old myocardial infarction. Usefulness of low-dose dobutamine infusion. Circulation. 1996;94:1834–41.
Yamamoto Y, de Silva R, Rhodes CG, Iida H, Lammertsma AA, Jones T, et al. Noninvasive quantification of regional myocardial metabolic rate of oxygen by 15O2 inhalation and positron emission tomography. Experimental validation. Circulation. 1996;94:808–16.
Iida H, Rhodes CG, Araujo LI, Yamamoto Y, de Silva R, Maseri A, et al. Noninvasive quantification of regional myocardial metabolic rate for oxygen by use of 15O2 inhalation and positron emission tomography. Theory, error analysis, and application in humans. Circulation. 1996;94:792–807.
Shidahara M, Watabe H, Kim KM, Oka H, Sago M, Hayashi T, et al. Evaluation of a commercial PET tomograph-based system for the quantitative assessment of rCBF, rOEF and rCMRO2 by using sequential administration of 15O-labeled compounds. Ann Nucl Med. 2002;16:317–27.
Mintun MA, Raichle ME, Martin WR, Herscovitch P. Brain oxygen utilization measured with O-15 radiotracers and positron emission tomography. J Nucl Med. 1984;25:177–87.
Magata Y, Temma T, Iida H, Ogawa M, Mukai T, Iida Y, et al. Development of injectable O-15 oxygen and estimation of rat OEF. J Cereb Blood Flow Metab. 2003;23:671–6.
Temma T, Magata Y, Kuge Y, Shimonaka S, Sano K, Katada Y, et al. Estimation of oxygen metabolism in a rat model of permanent ischemia using positron emission tomography with injectable 15O-O2. J Cereb Blood Flow Metab. 2006;26:1577–83.
Temma T, Kuge Y, Sano K, Kamihashi J, Obokata N, Kawashima H, et al. PET O-15 cerebral blood flow and metabolism after acute stroke in spontaneously hypertensive rats. Brain Res. 2008;1212:18–24.
Watabe H, Jino H, Kawachi N, Teramoto N, Hayashi T, Ohta Y, et al. Parametric imaging of myocardial blood flow with 15O-water and PET using the basis function method. J Nucl Med. 2005;46:1219–24.
Iida H, Rhodes CG, de Silva R, Yamamoto Y, Araujo LI, Maseri A, et al. Myocardial tissue fraction–correction for partial volume effects and measure of tissue viability. J Nucl Med. 1991;32:2169–75.
Wienhard K, Dahlbom M, Eriksson L, Michel C, Bruckbauer T, Pietrzyk U, et al. The ECAT EXACT HR: performance of a new high resolution positron scanner. J Comput Assist Tomogr. 1994;18:110–8.
Kudomi N, Hayashi T, Teramoto N, Watabe H, Kawachi N, Ohta Y, et al. Rapid quantitative measurement of CMRO2 and CBF by dual administration of 15O-labeled oxygen and water during a single PET scan-a validation study and error analysis in anesthetized monkeys. J Cereb Blood Flow Metab. 2005;25:1209–24.
Alders DJ, Groeneveld AB, de Kanter FJ, van Beek JH. Myocardial O2 consumption in porcine left ventricle is heterogeneously distributed in parallel to heterogeneous O2 delivery. Am J Physiol Heart Circ Physiol. 2004;287:H1353–61.
Van Woerkens EC, Trouwborst A, Duncker DJ, Koning MM, Boomsma F, Verdouw PD. Catecholamines and regional hemodynamics during isovolemic hemodilution in anesthetized pigs. J Appl Physiol. 1992;72:760–9.
Temma T, Magata Y, Iida H, Hayashi T, Ogawa M, Mukai T, et al. Development of injectable O-15 oxygen and its application for estimation of OEF. International Congress Series, Quantitation in Biomedical Imaging with PET and MRI Proceedings of the International Workshop on Quantitation in Biomedical Imaging with PET and MRI. 2004;1265:262–65.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Temma, T., Iida, H., Hayashi, T. et al. Quantification of regional myocardial oxygen metabolism in normal pigs using positron emission tomography with injectable 15O-O2 . Eur J Nucl Med Mol Imaging 37, 377–385 (2010). https://doi.org/10.1007/s00259-009-1262-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00259-009-1262-2