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In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals

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Abstract

Purpose

In vivo quantification of radiopharmaceuticals has great potential as a tool in developing new drugs. We investigated the accuracy of in vivo quantification with multi-pinhole single-photon emission computed tomography (SPECT) in rats.

Methods

Fifteen male Lewis rats with different stages of renal dysfunction were injected with 50 MBq 99mTc-dimercaptosuccinic acid. Four to six hours after injection, SPECT of the kidneys was acquired with a new four-headed multi-pinhole collimator camera. Immediately after imaging the rats were sacrificed and the kidneys were counted in a gamma-counter to determine the absorbed activity. SPECT data were reconstructed iteratively and regions of interest (ROIs) were drawn manually. The absolute activity in the ROIs was determined.

Results

Uptake values ranging from 0.71% to 21.87% of the injected activity were measured. A very strong linear correlation was found between the determined activity in vivo and ex vivo (r 2=0.946; slope m=1.059).

Conclusion

Quantification in vivo using this multi-pinhole SPECT system is highly accurate.

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Acknowledgements

Support was provided by the Swiss National Science Foundation, the Novartis Foundation (Switzerland), the Dutch Organisation for Scientific Research (ZonMw) (the Netherlands) and the Alexander von Humboldt Foundation. The authors wish to thank all supporting personnel of the Department of Nuclear Medicine, Erasmus MC, Rotterdam, and especially Marleen Melis, for their expert help and effort.

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Authors and Affiliations

  1. Department of Nuclear Medicine, Erasmus MC Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands

    F. Forrer, R. Valkema, B. Bernard, E. Rolleman, E. P. Krenning & M. de Jong

  2. Central Institute for Electronics, Research Centre Jülich, Jülich, Germany

    N. U. Schramm & J. W. Hoppin

Authors
  1. F. Forrer
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  2. R. Valkema
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  3. B. Bernard
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  4. N. U. Schramm
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  5. J. W. Hoppin
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  6. E. Rolleman
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  7. E. P. Krenning
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  8. M. de Jong
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Corresponding author

Correspondence to F. Forrer.

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Forrer, F., Valkema, R., Bernard, B. et al. In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals. Eur J Nucl Med Mol Imaging 33, 1214–1217 (2006). https://doi.org/10.1007/s00259-006-0178-3

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  • DOI: https://doi.org/10.1007/s00259-006-0178-3

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