Advertisement

Applied Microbiology and Biotechnology

, Volume 57, Issue 3, pp 342–345 | Cite as

Optimal design for the maximization of Penicillium cyclopium lipase production

  •  G. Vanot
  •  V. Deyris
  •  M.-C. Guilhem
  • Phan R. Luu
  •  L.-C. Comeau
Short Contribution

Abstract.

Penicillium cyclopium triacylglycerol lipase production was maximized in stationary batch culture. We used a surface response methodology based on a Doehlert experimental design to study the effect on the lipase activity released in the culture medium of the three most important factors: substrate concentration, pH and inoculum. Besides reducing the number of experiments required for optimization, this technique allowed us to quantify the lipase activity in any part of the experimental domain.We determined an optimal set of conditions for high lipase production: 1% substrate (corn steep), pH 5.5 and an inoculum of 104 spores/ml. Between conditions giving the minimum and the maximum lipase production, we observed a nine-fold increase of both the predicted and measured values.

Keywords

Corn Lipase Surface Response Triacylglycerol Surface Response Methodology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  G. Vanot
    • 1
  •  V. Deyris
    • 1
  •  M.-C. Guilhem
    • 2
  • Phan R. Luu
    • 2
  •  L.-C. Comeau
    • 1
  1. 1.Laboratoire de Chimie Biologique Appliquée, UDESAM, Faculté des Sciences et Techniques de St-Jérôme, Service 431, Avenue Escadrille Normandie-Niemen 13397 Marseille, Cedex 20, France
  2. 2.Laboratoire de la Méthodologie de la Recherche Expérimentale, UDESAM, Faculté des Sciences et Techniques de St-Jérôme, Avenue Escadrille Normandie-Niemen 13397 Marseille, Cedex 20, France

Personalised recommendations