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Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori

Applied Microbiology and Biotechnology volume 49, pages 393–398 (1998)Cite this article

Abstract

A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var. awamori strain NRRL312. Several fungal strains capable of synthesizing and secreting thaumatin into the culture medium were generated, and their production capabilities were determined, first in shake flasks and later in a laboratory fermentor. We report the expression and secretion of thaumatin in concentrations of 5–7 mg/l. This recombinant thaumatin is sweet.

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Affiliations

  1. URQUIMA, S.A. (URIACH Group), Arnau de Vilanova 22–42, E-08015 Sant Fost de Campsentelles (Barcelona), Spain Tel.: +34 3 570 5450 Fax: +34 3 570 2476, ES

    I. Faus, J. L. del Río, H. Sisniega & J. Bladé

  2. Centro Nacional de Biotecnología (CSIC-UAM), Campus Canto Blanco, E-28049 Madrid, Spain, ES

    C. del Moral, C. Patiño & V. Rubio

  3. Unitat d'Enginyería Química, Facultat de Ciencies, Universitat Autónoma de Barcelona, 08193 Bellaterra (Barcelona), Spain, ES

    N. Adroer & C. Casas

Authors
  1. I. Faus
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  2. C. del Moral
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  3. N. Adroer
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  4. J. L. del Río
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  5. C. Patiño
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  6. H. Sisniega
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  7. C. Casas
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  8. J. Bladé
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  9. V. Rubio
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Additional information

Received: 7 October 1997 / Received revision: 21 November 1997 / Accepted: 21 November 1997

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Faus, I., del Moral, C., Adroer, N. et al. Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori . Appl Microbiol Biotechnol 49, 393–398 (1998). https://doi.org/10.1007/s002530051188

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  • DOI: https://doi.org/10.1007/s002530051188

Keywords

  • Aspergillus
  • Aspergillus Niger
  • Filamentous Fungus
  • Shake Flask
  • Fungal Strain