The effects of dehydration/rehydration on two strains of Saccharomyces cerevisiae: S600, a metabolically engineered xylose-utilising strain, and H158, the non-xylose-utilising host strain; and on the naturally xylose-utilising yeast Pachysolen tannophilus CBS 4044, were compared after glucose and xylose utilisation respectively. The yeast strains differed in their ability to excrete and accumulate intracellular xylitol. A high intracellular xylitol content before and after dehydration coincided with a higher viability after a dehydration/rehydration cycle. The intracellular trehalose content increased during dehydration in all three yeast strains, but this did not correspond to enhanced cell viability after dehydration/rehydration. The results are discussed in relation to the ability of xylitol and trehalose to structure water.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Received: 9 July 1996 / Received revision: 29 October 1996 / Accepted: 2 November 1996
About this article
Cite this article
Krallish, I., Jeppsson, H., Rapoport, A. et al. Effect of xylitol and trehalose on dry resistance of yeasts. Appl Microbiol Biotechnol 47, 447–451 (1997). https://doi.org/10.1007/s002530050954
- Saccharomyces Cerevisiae