Abstract
In order to explore the relationship between sclerotial formation and antioxidant enzymes under abiotic stresses, the effects of abiotic stresses including temperature, pH value, osmotic pressure, limited nitrogen, and hydrogen peroxide (H2O2) on the activities of antioxidant enzymes, ascorbate peroxidase (APX), superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in Pleurotus tuber-regium were studied. Meanwhile, the sclerotial formation under these abiotic stress conditions was also investigated. It was found that low temperature, weak alkaline, appropriate osmotic stress, and H2O2 can promote sclerotial formation, and sclerotial formation always tended to occur when the activities of antioxidant enzymes were at a high value. During the prolonged low temperature stress, SOD acted mainly in the early stage of stress, while POD and CAT had higher activity in the middle and late stage. Moreover, the reverse transcription quantitative polymerase chain reaction (RT-qPCR) results showed that SOD.193 and POD.535 were significantly down-regulated in sclerotia, and CAT.1115 and POD.401 were up-regulated instead. These antioxidant enzyme genes played an important role in the sclerotial formation under low temperature stress. It is strongly suggested that antioxidant enzymes and abiotic stresses are closely related to sclerotial formation in P. tuber-regium.
Key points
• Low temperature and H2O2 can promote sclerotial formation.
• Sclerotia are more likely to form under high antioxidant enzyme activity.
• POD.401, POD.535, SOD.193, and CAT.1115 are important for sclerotial formation.
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This research was supported by a grant from the National Natural Science Foundation of China (31772375) to Aimin Ma.
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All authors contributed to this study. LS and AM conceived and designed experiments. LS conducted the research and wrote the manuscript. XS, CM, MH, XW, and AM critically reviewed the manuscript.
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Sheng, L., Sun, X., Mo, C. et al. Relationship between antioxidant enzymes and sclerotial formation of Pleurotus tuber-regium under abiotic stress. Appl Microbiol Biotechnol 107, 1391–1404 (2023). https://doi.org/10.1007/s00253-022-12358-3
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DOI: https://doi.org/10.1007/s00253-022-12358-3