Abstract
Combinations of various strategic approaches to the suppression of methanogenesis and the formation of biogas with a simultaneous decrease in the ratio of methane in its composition were investigated. Introduction of methanogenesis suppressors such as redox derivatives of humic acids, potassium persulfate (K2S2O8), possessing oxidizing and electron acceptor properties, enzyme hexahistidine-containing organophosphorus hydrolase with high lactonase activity and polypeptide antimicrobial agent bacitracin into the media with anaerobic consortia were studied. The effect of these substances was directed at various participants of the natural methanogenic consortium, as well as on the biochemical processes carried out by them. The use of K2S2O8 together with bacitracin provided maximum and almost complete suppression of CH4 production. The measured concentration of intracellular adenosine triphosphate has shown that viability of cells in the consortium remained almost the same, whereas their metabolic activity decreased. Various combinations of the above-mentioned suppressors provided different degrees of methanogenesis suppression, but redox agents played a key role in all the cases studied. Based on the accumulated data, combining suppressors in different concentrations can be used to manage the methanogenesis (efficiency and velocity of its decrease) in media with anaerobic consortia.
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Key points
• Various strategies for suppression of the methanogenesis were combined.
• The enzyme His6-OPH was firstly used for quorum quenching in methanogenic consortium.
• Velocity of methanogenesis decrease can be managed by combinations of suppressors.
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All datasets generated during this study are included in this article and its supplementary information files.
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Acknowledgements
This research was performed according to the Development program of the Interdisciplinary Scientific and Educational School of Lomonosov Moscow State University “The future of the planet and global environmental change.”
Funding
This research was funded by the Russian Foundation for Basic Research [Grant No. 18–29-25065]. The research was funded by State Task of Lomonosov Moscow State University (121041500039–8) in a part of His6-OPH obtaining and activity investigation and State Task of Lomonosov Moscow State University (12204060057–3) in a part of chemical modification of HS.
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E.E. and I.P. were involved in research conceptualization and formal analysis; N.S., O.S., A. Zh., and A.V. were responsible for methodology; O.S, I.P., A.V., and A. Zh. contributed to resources, N.S., O.S. and A. Zh. conducted investigation; O.S., N.S., O.M., E.E, and I.P. carried out data curation; N.S., O.S., and O.M. wrote and prepared the original draft; E.E. wrote, reviewed, and edited the manuscript; I.P. took part in supervision. All authors have read and agreed to the published version of the manuscript.
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Efremenko, E., Stepanov, N., Senko, O. et al. Strategies for variable regulation of methanogenesis efficiency and velocity. Appl Microbiol Biotechnol 106, 6833–6845 (2022). https://doi.org/10.1007/s00253-022-12148-x
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DOI: https://doi.org/10.1007/s00253-022-12148-x