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A method for the analysis of methylmercury and total Hg in fungal matrices

A Correction to this article was published on 26 July 2022

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Abstract

The aim of the study was to develop an efficient method for the determination of monomethyl-mercury (MeHg) and total mercury (THg) content in materials such as fungal sporocarps and sclerotia. Certified Reference Materials (CRMs) with the assigned values of MeHg and THg as well as the control materials (dried mushrooms) with known content of THg were evaluated for method validation. Recovery of MeHg from reference materials was at the following levels: from tuna fish at 87.0 ± 2.3% (THg at 101.9 ± 1.2%), from fish protein at 99.4 ± 1.3% (THg at 92.70 ± 0.41%), and from dogfish liver at 96.45 ± 0.73%. Recovery of THg from the fungal control material CS-M-5 was at 104.01 ± 0.60% (contribution of MeHg in THg content was at 6.2%), from CS-M-4 at 101.1 ± 2.0% (contribution at 3.2%), from CS-M-3 at 100.55 ± 0.67% (contribution at 0.6%), and from CS-M-2 at 101.5 ± 2.7% (contribution at 3.7%). The content of MeHg in randomly selected wild fungi and their morphological parts was in the range from 0.006 to 0.173 mg kg−1 dry weight (dw). In the case of THg, the concentration values were in the range from 0.0108 to 10.27 mg kg−1 dw. The MeHg content in the control materials with the assigned THg values was determined. Since the control materials play an important role in all elements of the quality assurance system of measurement results, they can be used to analyse MeHg as the first control material for fungi.

Key points

An extraction procedure for MeHg analysis in fungi was developed and optimized.

Recovery of MeHg from the certified reference non-fungal materials was > 87%.

Fungal control materials with assigned THg concentration can serve also for MeHg analysis.

Graphical abstract

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Fig. 1

Data availability

All data generated or analysed during this study are included in this published article.

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Acknowledgements

Donation of the polypore samples (Albatrellopsis ellisii and Jahnoporus hirtus) by Dr. Tjakko Stijve is acknowledged.

Funding

This study had part financial support from the National Science Centre under grant no. UMO-2016/23/N/NZ9/02746.

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Contributions

MR: Conceptualization, contributed reagents, performed the experiments, analyzed and interpreted data, drawn figures, wrote, reviewed, and edited the paper. JF: Conceptualization, contributed materials, analyzed and interpreted data, wrote, reviewed, and edited the paper, supervision. MS: Performed the experiments, contributed materials and reagents, help with quaerenda. PS: Help with quaerenda; reviewed the paper. M M-S: Performed the experiments, help with quaerenda. PK: Contributed reagents and analysis tools, reviewed the paper, and supervision.

Corresponding author

Correspondence to Jerzy Falandysz.

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This manuscript is dedicated to the memory of Dr. Tjakko Stijve (1937–2020).

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Rutkowska, M., Falandysz, J., Saba, M. et al. A method for the analysis of methylmercury and total Hg in fungal matrices. Appl Microbiol Biotechnol 106, 5261–5272 (2022). https://doi.org/10.1007/s00253-022-12043-5

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Keywords

  • Environment
  • Fungi
  • Macromycetes
  • Mushrooms
  • Food toxicology
  • Sclerotium
  • Trace elements