Abstract
Claudin-2 is a major component of tight junctions (TJs), which play an important role in reovirus entry into host cells. The Bombyx mori cytoplasmic polyhedosis virus (BmCPV) relates to the cypovirus strain of the reovirus family. So far, the role of claudin-2 in the process of BmCPV infection is not known. In the present study, it was observed that increasing expression of the claudin-2 gene (CLDN2) may concomitantly elevate BmCPV infection. Contrarily, knockdown of CLDN2 expression by siRNAs can reduce BmCPV infection. Similarly, antibody-based blockage of claudin-2 could also decrease BmCPV cell entry. These results suggest that claudin-2 can promote BmCPV infection in vitro. Moreover, immunofluorescence (IF) assays showed that claudin-2 can interact with BmCPV during viral infection. Specifically, co-immunoprecipitation experiments indicated that claudin-2 binds the BmCPV VP7 (instead of VP3 proteins). The interaction between VP7 and claudin-2 was further confirmed by bimolecular fluorescence complementation (BIFC). Altogether, our results suggest that BmCPV cell entry can be promoted upon interaction of VP7 with claudin-2. These findings provide new mechanistic insights related to BmCPV infection.
Key points
•Claudin-2 could promote BmCPV infection of cells.
•Claudin-2 interacted with BmCPV during BmCPV infection.
•Claudin-2 could interact with BmCPV VP7 protein, but not with VP3 proteins.
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This study was funded by the National Key R&D Program of China (2019YFA0905200), the National Natural Science Foundation of China (31872424, 31272500, 31972620, and 31602007), China Postdoctoral Science Foundation (2019M661937, 2019M651952), and Priority Academic Program of Development of Jiangsu Higher Education Institutions.
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C. L Gong contributed to the study conception and design. M Zhu and J Pan conducted experiments. M Zhu, X Zhang, J Pan, and H. X Zhu analyzed data. The first draft of the manuscript was written by M Zhu and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Zhu, M., Zhang, X., Pan, J. et al. Tight junction protein claudin-2 promotes cell entry of Bombyx mori cypovirus. Appl Microbiol Biotechnol 105, 6019–6031 (2021). https://doi.org/10.1007/s00253-021-11456-y
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DOI: https://doi.org/10.1007/s00253-021-11456-y