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White spot syndrome virus (WSSV) disturbs the intestinal microbiota of shrimp (Penaeus vannamei) reared in biofloc and clear seawater

  • Applied microbial and cell physiology
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Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

White spot syndrome virus (WSSV) is one of the most virulent pathogens afflicting shrimp farming. Understanding its influence on shrimp intestinal microbiota is paramount for the advancement of aquaculture, since gut dysbiosis can negatively impact shrimp development, physiology, and immunological response. Thereupon, the data presented herein assesses the influence of WSSV infection and different rearing systems on the intestinal microbiota of Penaeus vannamei. Our study aimed to describe and correlate the composition of shrimp (Penaeus vannamei) gut microbiota, when reared in biofloc and clear seawater, before and (48 h) after WSSV experimental infection. Shrimp were kept in two different systems (biofloc and clear seawater) and experimentally infected with WSSV. Intestine and water samples were characterized by 16S rRNA gene sequencing, before and after viral infection. We observed (i) WSSV induced higher mortality among shrimp reared in biofloc; (ii) WSSV led to a loss of intestinal microbiota heterogeneity, at the genus level, in shrimp kept in clear seawater; (iii) there was a prevalence of Cetobacterium and Bacillus in the intestine of shrimp from both systems; (iv) WSSV did not cause significant changes in intestinal microbiota diversity or richness; (v) regardless of the type of system and time of infection, intestinal microbiota was dissimilar to that of the surrounding water, despite being influenced by the type of system. Therefore, WSSV infection leads to punctual dysbiotic changes in shrimp microbiota, although the virus is sufficiently virulent to cause high mortalities even in well-managed systems, such as a balanced experimental biofloc system.

Key points

• WSSV infection leads to a perturbed gut microbiota in shrimp.

• WSSV infection greater impacts microbiota of shrimp reared in CSW than those in BFT.

• WSSV infection caused higher mortality levels in shrimp reared in BFT than in CSW.

• Rearing system influences shrimp gut microbiota composition.

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Data availability

The authors disclose that all data supporting the findings presented in the manuscript are available within the manuscript and supplemental material.

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Acknowledgments

We thank the staff from the Marine Shrimp Laboratory (UFSC, Brazil) for their daily assistance during the experiment and staff from the School of Biological & Marine Science (University of Plymouth, UK) for their support on molecular analysis.

Funding

This work was supported by the Brazilian official funding agency, The National Council for Scientific and Technological Development – CNPq, Proc 407211/2012-8; Proc 311099/2012-0.

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  1. Ana Rodiles

    Present address: Lallemand Animal Nutrition, Lallemand SAS, 31702, Blagnac, France

Authors and Affiliations

  1. Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, SC, Brazil

    Cecília de Souza Valente & Maria Risoleta Freire Marques

  2. School of Biological & Marine Sciences, Faculty of Science & Engineering, University of Plymouth, Plymouth, UK

    Ana Rodiles & Daniel Lee Merrifield

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  1. Cecília de Souza Valente
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Contributions

CSV and MRFM conceived and designed the research. CSV and MRFM conducted experiments. MRFM and DM provided funding. CSV, AR, MRFM, and DM analyzed the data. The first draft of the manuscript was written by CSV and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Cecília de Souza Valente.

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CSV received a CAPES (Coordination of Superior Level Staff Improvement) scholarship from the Aquaculture Graduate Program at UFSC. MRFM is the recipient of a CNPq (National Council for Scientific and Technological Development) productivity research grant. AR and DLM declare no conflict of interest. This work was part of the first author’s doctoral studies.

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de Souza Valente, C., Rodiles, A., Freire Marques, M.R. et al. White spot syndrome virus (WSSV) disturbs the intestinal microbiota of shrimp (Penaeus vannamei) reared in biofloc and clear seawater. Appl Microbiol Biotechnol 104, 8007–8023 (2020). https://doi.org/10.1007/s00253-020-10816-4

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