Abstract
Sur7 is one of multiple proteins constituting MCC (membrane compartment of Can1 acting as an arginine/H+ symporter), a crucial membrane domain that can form punctuate eisosome spots on the plasma membrane and execute diverse functions in model yeast but remains poorly understood in filamentous fungi. Here, a Sur7 homolog bearing a typical SUR7 domain and four transmembrane domains was shown to localize in the conidial vesicles and enter vacuoles and appear sporadically on the periphery membrane during hyphal growth in the insect-pathogenic fungus Beauveria bassiana, implicating an involvement of Sur7 in cellular events linked to both plasma membrane and vacuoles. Deletion of sur7 resulted in reduced conidiation capacity and impaired conidial quality, which was featured by slower germination, attenuated virulence, and reduced carbohydrate epitopes (β-N-acetylglucosamine and sialic acids). Also, the hyphal cell walls of the deletion mutant were severely impaired due to ~ 70% reductions in chitin and neutral carbohydrate contents and a moderate increase in alkali-soluble carbohydrate content. Consequently, the deletion mutant became more sensitive to three cell wall perturbing chemicals (Congo red, calcofluor white, and SDS) and an antifungal drug (caspofungin) and surprisingly showed a hypersensitivity to oxidative stress of H2O2 and an increased sensitivity to osmotic stress of NaCl or sorbitol. Its hypersensitivity to H2O2 was associated with transcriptional repression of critical catalase genes required for H2O2 decomposition. These findings unveil that Sur7 takes part in both MCC/eisosome and vacuolar events and hence acts as a sustainer of conidiation capacity, cell wall integrity, multiple stress tolerance, and virulence in B. bassiana.
Key points • Sur7 is a component of the crucial membrane domain MCC in Beauveria bassiana. • Sur7 localizes mainly in the vacuoles and sporadically on the periphery membrane. • Sur7 is required for cell wall integrity and has a pleiotropic effect on B. bassiana. |
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Acknowledgments
We thank She-Long Zhang (Core Facilities, College of Life Sciences, ZJU), Jun-Ying Li (Analytical Center of Agrobiology and Environmental Sciences, ZJU), and Ying-Ying Huang (Core Facilities, School of Medicine, ZJU) for technical assistance with LSCM, TEM, and flow cytometry analyses respectively.
Funding
Funding of this work was provided by the National Natural Science Foundation of China (Grant Nos.: 81701132, 31772218, and 31701844), the Ministry of Science and Technology of the People’s Republic of China (Grant No.: 2017YFD0201202), and Natural Science Foundation of Fujian (Grant No.: 2018 J05058).
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LBZ, LT, and MGF conceived and designed research. LBZ, LT, and YG conducted experiments. MGF contributed new reagents or analytical tools. LBZ and MGF analyzed data. LBZ and MGF wrote the manuscript. All authors read and approved the manuscript.
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Zhang, LB., Tang, L., Guan, Y. et al. Subcellular localization of Sur7 and its pleiotropic effect on cell wall integrity, multiple stress responses, and virulence of Beauveria bassiana. Appl Microbiol Biotechnol 104, 6669–6678 (2020). https://doi.org/10.1007/s00253-020-10736-3
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DOI: https://doi.org/10.1007/s00253-020-10736-3