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Effect of Rhizophora apiculata plantation for improving water quality, growth, and health of mud crab

  • Environmental biotechnology
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Abstract

A deteriorated water quality is closely associated with disease outbreaks in aquaculture, where microorganisms play indispensable roles in improving water quality and aquatic animals’ health. Mangrove is known to be a natural water quality filter and microbiological buffer of pathogen and prebiotics. However, it is unclear how and to what extent Rhizophora apiculata plantation is of benefits to the gut microbiota and growth over mud crab (Scylla paramamosain) aging. To address these concerns, we explored the bacterial communities in mud crab gut and rearing water at 45, 114, and 132 days after incubation, roughly corresponding to juvenile, pre-adult, and adult stages of mud crab. Results showed that 1-year R. apiculata plantation slightly increased the body weight of mud crab and improved water quality to a certain extent. Both bacterioplankton and gut bacterial communities were highly temporal dynamic, while the two communities were significantly distinct (ANOSIM r = 0.90, P = 0.0001). Relative abundances of dominant taxa in water and gut significantly varied between the plantation and the control conditions over mud crab aging. R. apiculata plantation promoted the stability of gut microbiota, as evidenced by more diverse core species. Furthermore, R. apiculata plantation led to the dominance of Verrucomicrobiae species in water and probiotic Bacteroidetes and Lactobacillales taxa in gut. A structural equation model revealed that water variables directly constrained gut microbiota, which in turn affected the body weight of mud crab (r = 0.52, P < 0.001). In addition, functional pathways facilitating immunity and lipid metabolism significantly increased in mud crab gut under the plantation, while those involved in infectious diseases exhibited the opposing trend. These findings greatly expand our understanding of the R. apiculata plantation effects on water quality, gut microbiota, and growth feature of mud crab. Overall, R. apiculata plantation is beneficial for mud crab growth and health.

Key Points

• A short-term R. apiculata plantation could potentially improve water quality.

• Bacterioplankton is more sensitive than mud crab gut microbiota in response to R. apiculata plantation.

• R. apiculata plantation enhances mud crab resistance against pathogen invasion.

• R. apiculata plantation alters mud crab gut microbiota, which in turn promotes their body weight.

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Funding

This work was supported by the China Agriculture Research System (CARS-48), the Natural Science Fund for Distinguished Young Scholars of Zhejiang Province (LR19C030001), and the K.C. Wong Magna Fund in Ningbo University.

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Authors and Affiliations

  1. School of Marine Sciences, Ningbo University, Ningbo, 315211, China

    Wenfang Dai, Jinbo Xiong, Hao Zheng, Sui Ni, Yangfang Ye & Chunlin Wang

  2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China

    Wenfang Dai & Jinbo Xiong

  3. Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, 315211, China

    Yangfang Ye & Chunlin Wang

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  1. Wenfang Dai
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  2. Jinbo Xiong
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  3. Hao Zheng
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  4. Sui Ni
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  5. Yangfang Ye
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  6. Chunlin Wang
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Contributions

J. Xiong and C. Wang designed the study. W. Dai, H. Zheng, S. Ni, and Y. Ye performed the experiments. W. Dai and J. Xiong analyzed the data and wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Jinbo Xiong or Chunlin Wang.

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This article does not contain any studies with human participants performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed, as well as the National Institutes of Health Guide for the Care and Use of Laboratory Animals.

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Dai, W., Xiong, J., Zheng, H. et al. Effect of Rhizophora apiculata plantation for improving water quality, growth, and health of mud crab. Appl Microbiol Biotechnol 104, 6813–6824 (2020). https://doi.org/10.1007/s00253-020-10716-7

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  • DOI: https://doi.org/10.1007/s00253-020-10716-7

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