Skip to main content
Log in

Two-stage oxygen supply strategy for enhancing fed-batch production of pyrroloquinoline quinone in Hyphomicrobium denitrificans FJNU-6

  • Biotechnological products and process engineering
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Oxygen is a vital parameter for pyrroloquinoline quinone (PQQ) biosynthesis. In this study, the effects of oxygen supply on the biosynthesis of PQQ were first investigated systematically with Hyphomicrobium denitrificans FJNU-6. Following a kinetic analysis of the specific cell growth rate (μx) and specific PQQ formation rate (μp) in 5 L benchtop fermentation systems at various oxygen supply levels ranging from 0 to 60%, a novel, two-stage oxygen supply strategy was developed for enhancing PQQ production and productivity. Moreover, the transcription of genes involved in methanol oxidation and PQQ biosynthesis was analyzed throughout the process to outline the effect of oxygen supply on cell metabolism. Furthermore, with constant feeding of methanol at 0–1 g/L after the initial methanol was consumed completely, the PQQ concentration and productivity reached 1070 mg/L and 7.64 mg/L/h, respectively, after 140 h in a 5-L fermenter. The two-stage oxygen supply strategy developed in this study provides an effective and economical strategy for the industrial production of PQQ.

Key Points

• A novel, two-stage oxygen supply strategy was developed for enhancing PQQ production and productivity.

•The transcription of genes involved in methanol oxidation and PQQ biosynthesis was regulated by changes in oxygen supply.

• This study offers an effective and economical strategy for industrial or large-scale production of PQQ.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

Download references

Funding

This study was funded by grants from the National Natural Science Foundation of China (Grant No. 21807011), Natural Science Foundation of Fujian Province (Grant No. 2018J01673), and the Special Scientific Research Fund of the Higher Education Institutions of Fujian Province (Grant No. JK2017012).

Author information

Authors and Affiliations

Authors

Contributions

KCR and HJZ conceived and designed the research. LMS, RY, and XHP conducted the experiments. YXW, LMS, and KCR analyzed the data. LMS and YXW wrote the manuscript. All authors read and approved the manuscript.

Corresponding authors

Correspondence to Jianzhong Huang or Chongrong Ke.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(PDF 259 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, M., Yang, X., Ren, Y. et al. Two-stage oxygen supply strategy for enhancing fed-batch production of pyrroloquinoline quinone in Hyphomicrobium denitrificans FJNU-6. Appl Microbiol Biotechnol 104, 6615–6622 (2020). https://doi.org/10.1007/s00253-020-10690-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-020-10690-0

Keywords

Navigation