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Biotechnological advancements in Catharanthus roseus (L.) G. Don

Applied Microbiology and Biotechnology volume 104, pages 4811–4835 (2020)Cite this article

Abstract

Catharanthus roseus (L.) G. Don, also known as Madagascar periwinkle or Sadabahar, is a herbaceous plant belonging to the family Apocynaceae. Being a reservoir for more than 200 alkaloids, it reserves a place for itself in the list of important medicinal plants. Secondary metabolites are present in its leaves (e.g., vindoline, vinblastine, catharanthine, and vincristine) as well as basal stem and roots (e.g., ajmalicine, reserpine, serpentine, horhammericine, tabersonine, leurosine, catharanthine, lochnerine, and vindoline). Two of its alkaloids, vincristine and vinblastine (possessing anticancerous properties), are being used copiously in pharmaceutical industries. Till date, arrays of reports are available on in vitro biotechnological improvements of C. roseus. The present review article concentrates chiefly on various biotechnological advancements based on plant tissue culture techniques of the last three decades, for instance, regeneration via direct and indirect organogenesis, somatic embryogenesis, secondary metabolite production, synthetic seed production, clonal fidelity assessment, polyploidization, genetic transformation, and nanotechnology. It also portrays the importance of various factors influencing the success of in vitro biotechnological interventions in Catharanthus and further addresses several shortcomings that can be further explored to create a platform for upcoming innovative approaches.

Key Points

• C. roseus yields anticancerous vincristine and vinblastine used in pharma industry.

• In vitro biotechnological interventions prompted major genetic advancements.

• This review provides an insight on in vitro-based research achievements till date.

• Key bottlenecks and prospective research methodologies have been identified herein.

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Acknowledgments

Authors acknowledge the University e-library assistance and the experimental assistance from Plant Tissue Culture laboratory at Regional Nuclear Agricultural Research Centre, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India. We are further thankful to the anonymous reviewer(s) and the editor of this article for their critical comments and suggestions on the manuscript.

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Authors and Affiliations

  1. Crop Research Unit (Genetics and Plant Breeding), Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, 741252, India

    Anamika Das, Sutanu Sarkar, Somnath Bhattacharyya & Saikat Gantait

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  1. Anamika Das
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  2. Sutanu Sarkar
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  3. Somnath Bhattacharyya
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  4. Saikat Gantait
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Contributions

SG conceived the idea of the review. AD and SG surveyed the literature and drafted the initial manuscript. SG, SS, and SB scrutinized and corrected the manuscript to its final version. All the authors read and approved the final version of the manuscript prior to its submission.

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Correspondence to Saikat Gantait.

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Das, A., Sarkar, S., Bhattacharyya, S. et al. Biotechnological advancements in Catharanthus roseus (L.) G. Don. Appl Microbiol Biotechnol 104, 4811–4835 (2020). https://doi.org/10.1007/s00253-020-10592-1

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  • DOI: https://doi.org/10.1007/s00253-020-10592-1

Keywords

  • Anticancerous
  • Genetic transformation
  • Nanotechnology
  • Polyploidy
  • Secondary metabolite
  • Somatic embryogenesis