Skip to main content
SpringerLink
Log in

Biotechnological advancements in Catharanthus roseus (L.) G. Don

  • Mini-Review
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Catharanthus roseus (L.) G. Don, also known as Madagascar periwinkle or Sadabahar, is a herbaceous plant belonging to the family Apocynaceae. Being a reservoir for more than 200 alkaloids, it reserves a place for itself in the list of important medicinal plants. Secondary metabolites are present in its leaves (e.g., vindoline, vinblastine, catharanthine, and vincristine) as well as basal stem and roots (e.g., ajmalicine, reserpine, serpentine, horhammericine, tabersonine, leurosine, catharanthine, lochnerine, and vindoline). Two of its alkaloids, vincristine and vinblastine (possessing anticancerous properties), are being used copiously in pharmaceutical industries. Till date, arrays of reports are available on in vitro biotechnological improvements of C. roseus. The present review article concentrates chiefly on various biotechnological advancements based on plant tissue culture techniques of the last three decades, for instance, regeneration via direct and indirect organogenesis, somatic embryogenesis, secondary metabolite production, synthetic seed production, clonal fidelity assessment, polyploidization, genetic transformation, and nanotechnology. It also portrays the importance of various factors influencing the success of in vitro biotechnological interventions in Catharanthus and further addresses several shortcomings that can be further explored to create a platform for upcoming innovative approaches.

Key Points

• C. roseus yields anticancerous vincristine and vinblastine used in pharma industry.

• In vitro biotechnological interventions prompted major genetic advancements.

• This review provides an insight on in vitro-based research achievements till date.

• Key bottlenecks and prospective research methodologies have been identified herein.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Almagro L, Perez AJL, Pedreno MA (2011) New method to enhance ajmalicine production in Catharanthus roseus cell cultures based on the use of cyclodextrins. Biotechnol Lett 33:381–385

    CAS  PubMed  Google Scholar 

  • Almagro L, Fernández-Pérez F, Pedreño MA (2015) Indole alkaloids from Catharanthus roseus: bioproduction and their effect on human health. Molecules 20:2973–3000

    PubMed  PubMed Central  Google Scholar 

  • Al-Oubaidi HK, Mohammed-Ameen AS (2014) Effect of benzyladenine on multiplication of Catharanthus roseus L. in vitro. World J Pharm Pharm Sci 3:2101–2107

    Google Scholar 

  • Al-Shmgani HSA, Mohammed WH, Sulaiman GM, Saadoon AH (2017) Biosynthesis of silver nanoparticles from Catharanthus roseus leaf extract and assessing their antioxidant, antimicrobial, and wound-healing activities. Artif Cells Nanomed Biotechnol 45:1–7

    PubMed  Google Scholar 

  • Aruna MS, Prabha MS, Priya NS, Nadendla R (2015) Catharanthus Roseus: ornamental plant is now medicinal boutique. J Drug Deliv Ther 5:1–4

    Google Scholar 

  • Aslam J, Mujib A, Fatima S, Sharma MP (2008) Cultural conditions affect somatic embryogenesis in Catharanthus roseus L. (G.) Don. Plant Biotechnol Rep 2:179–189

    Google Scholar 

  • Aslam J, Mujib A, Nasim SA, Sharma MP (2009) Screening of vincristine yield in ex vitro and in vitro somatic embryos derived plantlets of Catharanthus roseus L. (G) Don. Sci Hortic 119:325–329

    CAS  Google Scholar 

  • Aslam J, Khan SH, Siddiqui ZH, Fatima Z, Maqsood M, Bhat MA, Nasim SA, Ilah A, Ahmad IZ, Khan SA, Mujib A, Sharma MP (2010a) Catharanthus roseus (L.) G. Don. An important drug: it’s applications and production. Pharm Glob 4:1–16

    Google Scholar 

  • Aslam J, Mujib A, Fatima Z, Sharma MP (2010b) Variations in vinblastine production at different stages of somatic embryogenesis, embryo, and field-grown plantlets of Catharanthus roseus L. (G) Don, as revealed by HPLC. In Vitro Cell Dev Biol—Plant 46:348–353

    Google Scholar 

  • Ataei-Azimi A, Hashemloian BD, Ebrahimzadeh H, Majd A (2008) High in vitro production of ant-canceric indole alkaloids from periwinkle (Catharanthus roseus) tissue culture. Afr J Biotechnol 7:2834–2839

    CAS  Google Scholar 

  • Bakrudeen AAA, Shanthi GS, Gouthaman T, Kavitha MS, Rao MV (2011) In vitro micropropagation of Catharanthus roseus – an anticancer medicinal plant. Acta Bot Hung 53:197–209

    Google Scholar 

  • Baskar G, Sathivel K, George GB (2016) In vitro cytotoxicity of copper oxide nanobiocomposites synthesized by Catharanthus roseus flower extract against breast cancer cell line. J Chem Pharm Sci 9:211–214

    CAS  Google Scholar 

  • Begum T, Mathur M (2014) In vitro regeneration of Catharanthus roseus and Bacopa monnieri and their survey around Jaipur District. Int J Pure Appl Biosci 2:210–221

    Google Scholar 

  • Begum F, Rao SSSN, Rao K, Devi YP, Giri CC (2009) Increased vincristine production from Agrobacterium tumefaciens C58 induced shooty teratomas of Catharanthus roseus G. Don. Nat Prod Res 23:973–981

    CAS  PubMed  Google Scholar 

  • Bhadra R, Vani S, Shanks JV (1993) Production of indole alkaloids by selected hairy root lines of Catharanthus roseus. Biotechnol Bioeng 41:581–592

    CAS  PubMed  Google Scholar 

  • Binder BYK, Peeble CAM, Shanks JV, San KY (2009) The effects of UV-B stress on the production of terpenoid indole alkaloids in Catharanthus roseus hairy roots. Biotechnol Prog 25:861–865

    CAS  PubMed  Google Scholar 

  • Choi PS, Kim YD, Choi KM, Chung HJ, Choi DW, Liu JR (2004) Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus. Plant Cell Rep 22:828–831

    CAS  PubMed  Google Scholar 

  • Ciau-uitz R, Miranda-ham ML, Coello-Coello J, Chi B, Pacheco LM, Loyola-Vargas OVM (1994) Indole alkaloid production by transformed and non-transformed root cultures of Catharanthus roseus. In Vitro Cell Dev Biol—Plant 30:84–88

    Google Scholar 

  • Das S, Sharangi AB (2017) Madagascar periwinkle (Catharanthus roseus L.): diverse medicinal and therapeutic benefits to humankind. J Pharmacogn Phytochem 6:1695–1701

    CAS  Google Scholar 

  • Datta A, Srivastava PS (1997) Variation in vinblastine production by Catharanthus roseus during in vivo and in vitro differentiation. Phytochemistry 46:135–137

    CAS  Google Scholar 

  • Dhandapani M, Kim DH, Hong SB (2008) Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus. In Vitro Cell Dev Biol—Plant 44:18–25

    CAS  Google Scholar 

  • Dhawan OE, Lavania UC (1996) Enhancing the productivity of secondary metabolites via induced polyploidy: a review. Euphytica 87:81–89

    CAS  Google Scholar 

  • Filippini R, Caniato R, Vecchia FD, Cappelletti EM, Puricelli L, Piovan A, Innocenti G (2000) Somatic embryogenesis and indole alkaloid production in Catharanthus roseus. Plant Biosyst 134:179–184

    Google Scholar 

  • Gajalakshmi S, Vijayalakshmi S, Devi VR (2013) Pharmacological activities of Catharanthus Roseus: a perspective review. Int J Pharma Bio Sci 4:431–439

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soyabean root cells. Exp Cell Res 50:151–158

    CAS  PubMed  Google Scholar 

  • Gantait S, Kundu S (2017a) In vitro biotechnological approaches on Vanilla planifolia Andrews: advancements and opportunities. Acta Physiol Plant 39:196

    Google Scholar 

  • Gantait S, Kundu S (2017b) Neoteric trends in tissue culture-mediated biotechnology of Indian ipecac [Tylophora indica (Burm. f.) Merrill]. 3 Biotech 7:231

    PubMed  PubMed Central  Google Scholar 

  • Gantait S, Sinniah UR, Das PK (2014) Aloe vera: a review update on advancement of in vitro culture. Acta Agric Scand Sect B 64:1–12

    CAS  Google Scholar 

  • Gantait S, El-Dawayati MM, Panigrahi J, Labrooy C, Verma SK (2018) The retrospect and prospect of the applications of biotechnology in Phoenix dactylifera L. Appl Microbiol Biotechnol 102:8229–8259

    CAS  PubMed  Google Scholar 

  • Ghasempour M, Iranbakhsh A, Ebadi M, Ardebili ZO (2019) Multi-walled carbon nanotubes improved growth, anatomy, physiology, secondary metabolism, and callus performance in Catharanthus roseus: an in vitro study. 3 Biotech 9:404

    PubMed  Google Scholar 

  • Ghozali SZ, Vuanghao L, Ahmad NH (2015) Biosynthesis and characterization of silver nanoparticles using Catharanthus roseus leaf extract and its proliferative effects on cancer cell lines. J Nanomed Nanotechnol 6:4

    Google Scholar 

  • Guillon S, Trémouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006) Harnessing the potential of hairy roots: dawn of a new era. Trends Biotechnol 24:403–409

    CAS  PubMed  Google Scholar 

  • Guo Z, Liu Y, Gong M, Chen W, Li W (2013) Regulation of vinblastine biosynthesis in cell suspension cultures of Catharanthus roseus. Plant Cell Tissue Organ Cult 112:43–54

    CAS  Google Scholar 

  • Hanafy MS, Matter MA, Asker MS, Rady MR (2016) Production of indole alkaloids in hairy root cultures of Catharanthus roseus L. and their antimicrobial activity. S Afr J Bot 105:9–18

    CAS  Google Scholar 

  • Hernández-Domínguez E, Campos-Tamayo F, Vázquez-Flota F (2004) Vindoline synthesis in in vitro shoot cultures of Catharanthus roseus. Biotechnol Lett 26:671–674

    PubMed  Google Scholar 

  • Hilliou F, Christou P, Leech MJ (1999) Development of an efficient transformation system for Catharanthus roseus cell cultures using particle bombardment. Plant Sci 140:179–188

    CAS  Google Scholar 

  • Hirata K, Horiuchi M, Ando T, Miyamoto K, Miura Y (1990) Vindoline and catharanthine production in multiple shoot. J Ferment Bioeng 70:193–195

    CAS  Google Scholar 

  • Hirata K, Horiuchi M, Asada M, Ando T, Miyamoto K, Miura Y (1992) Stimulation of dimeric alkaloid production by near-ultraviolet light in multiple shoot cultures of Catharanthus roseus. J Ferment Bioeng 74:222–225

  • Hirata K, Miyamoto K, Miura Y (1994) Catharanthus roseus L. (Periwinkle): production of vindoline and catharanthine in multiple shoot cultures. In: Bajaj YPS (ed) Medicinal and aromatic plants VI. Biotechnology in Agriculture and Forestry, vol 26. Springer, Berlin, pp 46–55

    Google Scholar 

  • Hosseini HR, Chehrazi M, Sorestani MM, Ahmadi DN, Sorkhe K (2013) Autotetraploidy induction and seed quality comparison between diploid and tetraploid Madagascar periwinkle (Catharanthus roseus cv. rosea) seedlings. Int J Agron Plant Prod 4:212–216

    Google Scholar 

  • Hosseini HR, Chehrazi M, Ahmadi DN, Sorestani MM (2018) Colchicine-induced autotetraploidy and altered plant cytogenetic and morphophysiological traits in Catharanthus roseus (L.) G. Don. Adv Hort Sci 32:229–238

    Google Scholar 

  • Hughes EH, Hong SB, Gibson SI, Shanks JV, San KY (2004) Metabolic engineering of the indole pathway in Catharanthus roseus hairy roots and increased accumulation of tryptamine and serpentine. Metab Eng 6:268–276

    CAS  PubMed  Google Scholar 

  • Ilah A, Mujib A, Junaid A, Samar F, Abdin MZ (2009) Somatic embryogenesis and two embryo specific proteins (38 and 33 kD) in Catharanthus roseus. Biologia 64:299–304

    Google Scholar 

  • Jaleel CA, Gopi R, Gomathinayagam M, Panneerselvam R (2009) Traditional and non-traditional plant growth regulators alters phytochemical constituents in Catharanthus roseus. Process Biochem 44:205–209

    CAS  Google Scholar 

  • Junaid A, Bhatt MA, Mujib A, Sharma MP (2006) Somatic embryo proliferation, maturation and germination in Catharanthus roseus. Plant Cell Tissue Organ Cult 84:325–332

    Google Scholar 

  • Junaid A, Mujib A, Sharma MP, Samaj J (2007a) Somatic embryogenesis and plant regeneration in Catharanthus roseus. Biol Plant 51:641–646

    CAS  Google Scholar 

  • Junaid A, Mujib A, Sharma MP, Tang W (2007b) Growth regulators affect primary and secondary somatic embryogenesis in Madagaskar periwinkle (Catharanthus roseus (L.) G Don.) at morphological and biochemical levels. Plant Growth Regul 51:271–281

    CAS  Google Scholar 

  • Junaid A, Mujib A, Fatima S, Sharma MP (2008) Culture condition effect somatic embryogenesis in Catharanthus roseus L (G.) Don. Plant Biotechnol Rep 2:179–190

    Google Scholar 

  • Jung KH, Kwak SS, Kim SW, Lee H, Choi CY, Liu JR (1992) Improvement of the catharanthine productivity in hairy root cultures of Catharanthus roseus by using monosaccharides as a carbon source. Biotechnol Lett 14:695–700

    CAS  Google Scholar 

  • Kalidass C, Mohan VR, Daniel A (2010) Effect of auxin and cytokinin on vincristine production by callus cultures of Catharanthus roseus L. (Apocynaceae). Trop Subtrop Agroecosys 12:283–288

    Google Scholar 

  • Kaushik S, Tomar RS, Gupta M, Mishra RK (2017) An overview of Catharanthus roseus and medicinal properties of their metabolites against important diseases. Eur Acad Res 5:1237–1247

    Google Scholar 

  • Kim SW, Jung KH, Song NH, Kwak SS, Liu JR (1994) High frequency plant regeneration from anther-derived cell suspension cultures via somatic embryogenesis in Catharanthus roseus. Plant Cell Rep 13:319–322

    CAS  PubMed  Google Scholar 

  • Kim SW, In DS, Choi PS, Liu JR (2004) Plant regeneration from immature zygotic embryo-derived embryogenic calluses and cell suspension cultures of Catharanthus roseus. Plant Cell Tissue Organ Cult 76:131–135

    CAS  Google Scholar 

  • Kobza F, Qing L (2000) Ploidy increasing of Catharanthus roseus (L.) G. Don. by chemomutation. In XIX International Symposium on Improvement of Ornamental Plants 508, pp.347–348

  • Kulkarni RN, Baskaran K, Jhang T (2016) Breeding medicinal plant, periwinkle [Catharanthus roseus (L) G. Don]: a review. Plant Genet Resour C 14:283–302

    CAS  Google Scholar 

  • Kumar A, Prakash K, Sinha RK, Kumar N (2013) In vitro plant propagation of Catharanthus roseus and assessment of genetic fidelity of micropropagated plants by RAPD marker assay. Applied Biochem Biotechnol 169:894–900

    CAS  Google Scholar 

  • Le Roux M, Guéritte F (2017) From Catharanthus roseus alkaloids to the discovery of vinorelbine (Navelbine®). In: Navelbine® and Taxotère®, Elsevier, pp. 87–149

  • Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127

    CAS  Google Scholar 

  • Lloyd G, McCown B (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by shoot tip culture. Int Plant Prop Soc Proc 30:421–427

    Google Scholar 

  • Malabadi RB, Chalannavar RK, Meti NT, Mulgund GS, Nataraja K, Kumar SV (2012) Synthesis of antimicrobial silver nanoparticles by callus cultures and in vitro derived plants of Catharanthus roseus. Res Pharm 2:18–31

    CAS  Google Scholar 

  • Maqsood M, Mujib A, Siddiqui ZH (2012) Synthetic seed development and conversion to plantlet in Catharanthus roseus (L.) G. Don. Biotechnol 11:37–43

    CAS  Google Scholar 

  • Mehta J, Upadhyay D, Paras P, Ansari R, Rathore S, Tiwari S (2013) Multiple shoots regeneration of (anti-cancer plant) Catharanthus roseus - an important medicinal plant. American J Pharm Tech Res 3:785–793

    CAS  Google Scholar 

  • Mitra A, Khan B, Rawal S (1998) Rapid in vitro multiplication of plants from mature nodal explants of Catharanthus roseus. Planta Med 64:390

    CAS  PubMed  Google Scholar 

  • Mitra M, Gantait S, Mandal N (2020) Coleus forskohlii: advancements and prospects of in vitro biotechnology. Appl Microbiol Biotechnol 104:2359–2371

    CAS  PubMed  Google Scholar 

  • Moghe S, Laud D, Dehankar B, Moghe R, Sadhu P, Ade G, Ishani B, Hadke A (2016) Effect of growth regulator combination on in-vitro regeneration of Catharanthus roseus. Int J Life Sci 6:1–4

    Google Scholar 

  • Moreno PRH, Heijden RVD, Verpoorte R (1994) Elicitor-mediated induction of isochorismate synthase and accumulation of 2,3-dihydroxy benzoic acid in Catharanthus roseus cell suspension and shoot cultures. Plant Cell Rep 14:188–191

    CAS  PubMed  Google Scholar 

  • Mujib A, Ilah A, Aslam J, Fatima S, Siddiqui ZH, Maqsood M (2012) Catharanthus roseus alkaloids: application of biotechnology for improving yield. Plant Growth Regul 68:111–127

    CAS  Google Scholar 

  • Mukherjee E, Gantait S, Kundu S, Sarkar S, Bhattacharyya S (2019) Biotechnological interventions on the genus Rauvolfia: recent trends and imminent prospects. Appl Microbiol Biotechnol 103:7325–7354

    CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–495

    CAS  Google Scholar 

  • Nagaonkar D, Gaikwad S, Rai M (2015) Catharanthus roseus leaf extract-synthesized chitosan nanoparticles for controlled in vitro release of chloramphenicol and ketoconazole. Colloid Polym Sci 293:1465–1473

    CAS  Google Scholar 

  • O’Keefe BR, Mahady GB, Gills JJ, Beecher CWW (1997) Stable vindoline production in transformed cell cultures of Catharanthus roseus. J Nat Prod 60:261–264

    Google Scholar 

  • Omino EA (1996) The plant family Apocynaceae in East Africa. In: van der Maesen LJG, van der Burgt XM, van Medenbach de Rooy JM (eds) The biodiversity of African plants. Springer, Dordrecht, pp 504–506

    Google Scholar 

  • Osibe DA, Chiejina NV, Ogawa K, Aoyagi H (2018) Stable antibacterial silver nanoparticles produced with seed-derived callus extract of Catharanthus roseus. Artif Cells Nanomed Biotechnol 46:1266–1273

    CAS  PubMed  Google Scholar 

  • Panigrahi J, Dholu P, Shah TJ, Gantait S (2018) Silver nitrate-induced in vitro shoot multiplication and precocious flowering in Catharanthus roseus (L.) G. Don, a rich source of terpenoid indole alkaloids. Plant Cell Tissue Organ Cult 132:579–584

    CAS  Google Scholar 

  • Pati PK, Kaur J, Singh J (2011) A liquid culture system for shoot proliferation and analysis of pharmaceutically active constituents of Catharanthus roseus (L.) G. Don. Plant Cell Tissue and Organ Cult 105:299–307

    CAS  Google Scholar 

  • Pavunraj M, Baskar K, Duraipandiyan V, Al-Dhabi NA, Rajendran V, Benelli G (2017) Toxicity of Ag nanoparticles synthesized using stearic acid from Catharanthus roseus leaf extract against Earias vittella and mosquito vectors (Culex quinquefasciatus and Aedes aegypti). J Clust Sci 28:2477–2492

    CAS  Google Scholar 

  • Pietrosiuk A, Furmanowa M, Łata B (2007) Catharanthus roseus: micropropagation and in vitro techniques. Phytochem Rev 6:459–473

    CAS  Google Scholar 

  • Ponarulselvam S, Panneerselvam C, Murugan K, Aarthi N, Kalimuthu K, Thangamani S (2012) Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities. Asian Pac J Trop Biomed 2:574–580

    CAS  PubMed  PubMed Central  Google Scholar 

  • Rahmatzadeh S, Khara J, Kazemitabar SK (2014) The study of in vitro regeneration and growth parameters in Catharanthus roseus L. under application of tryptophan. J Sci Kharazmi Univ 14:249–260

    Google Scholar 

  • Raja A, Salique SM, Gajalakshmi P, James A (2016) Antibacterial and hemolytic activity of green silver nanoparticles from Catharanthus roseus. Int J Pharm Sci Nanotechnol 9:1–6

    Google Scholar 

  • Rajagopal T, Jemmiah IAA, Ponmanickam P, Ayyanar M (2015) Synthesis of silver nanoparticles using Catharanthus roseus leaf extract and its larvicidal effects. Environ Biol 36:1283–1289

    CAS  Google Scholar 

  • Rajora RK, Sharma NK, Sharma V (2013) Effect of plant growth regulators on micropropagation of Catharanthus roseus. Int J Adv Biotechnol Res 4:123–130

    Google Scholar 

  • Ramani S, Jayabaskaran C (2008) Enhanced catharanthine and vindoline production in suspension cultures of Catharanthus roseus by ultraviolet-B light. J Mol Signal 3:9

    PubMed  PubMed Central  Google Scholar 

  • Riaz HR, Hashmi SS, Khan T, Hano C, Giglioli-Guivarc’h N, Abbasi BH (2018) Melatonin-stimulated biosynthesis of antimicrobial ZnONPs by enhancing bio-reductive prospective in callus cultures of Catharanthus roseus var. Alba. Artif Cells Nanomed Biotechnol 46:936–950

    CAS  PubMed  Google Scholar 

  • Rizvi NF, Cornejo M, Stein K, Weaver J, Cram EJ, Lee-Parsons CWT (2015) An efficient transformation method for estrogen-inducible transgene expression in Catharanthus roseus hairy roots. Plant Cell Tissue Organ Cult 120:475–487

    CAS  Google Scholar 

  • Saifullah KS (2011) Callus induction and cell suspension culture production of catharanthus roseus for biotransformation studies of (−)- Caryophyllene oxide. Pak J Bot 43:467–473

    CAS  Google Scholar 

  • Salma U, Kundu S, Gantait S (2018) Conserving biodiversity of a potent anticancer plant, Catharanthus roseus, through in vitro biotechnological intercessions: substantial progress and imminent prospects. In: Akhtar MS, Swamy MK (eds) Anticancer plants: natural products and biotechnological implements, Vol 2. Springer Nature, Singapore, pp 83–107

    Google Scholar 

  • Satdive RK, Fulzele DP, Eapen S (2003) Studies on production of ajmalicine in shake flasks by multiple shoot cultures of Catharanthus roseus. Biotechnol Prog 19:1071–1075

    CAS  PubMed  Google Scholar 

  • Shala AY, Deng Z (2018) Investigation of morphological and anatomical changes in Catharanthus roseus (L.) G. Don due to colchicines induced polyploidy. Sci J Flower Ornam Plant 5:233–243

    Google Scholar 

  • Shanks JV, Morgan J (1999) Plant ‘hairy root’ culture. Curr Opin Biotechnol 10:151–155

    CAS  PubMed  Google Scholar 

  • Sharma V, Kaur H, Kumar T, Mishra T (2016) Traditional Indian herb Cathranthus roseus used as cancer treatment: a review. Int J Pharm Phytochem Res 8:1926–1928

    Google Scholar 

  • Sharma A, Mathur AK, Ganpathy J, Joshi B, Patel P (2019) Effect of abiotic elicitation and pathway precursors feeding over terpenoid indole alkaloids production in multiple shoot and callus cultures of Catharanthus roseus. Biologia 74:543–554

    CAS  Google Scholar 

  • Sheshadri SA, Sriram S, Balamurugan P, Anupriya R, Princy SA, Brindhab P, Bindu S (2015) Melatonin improves bioreductant capacity and silver nanoparticles synthesis using Catharanthus roseus leaves. RSC Adv 5:47548

    CAS  Google Scholar 

  • Shittu OK, Stephen DI, Kure AH (2017) Functionalization of biosynthesized gold nanoparticle from aqueous leaf extract of Catharanthus roseus for antibacterial studies. Afr J Biomed Res 20:195–202

    Google Scholar 

  • Shukla AK, Shasany AK, Verma RK, Gupta MM, Mathur AK, Khanuja SP (2010) Influence of cellular differentiation and elicitation on intermediate and late steps of terpenoid indole alkaloid biosynthesis in Catharanthus roseus. Protoplasma 242:35–47

    CAS  PubMed  Google Scholar 

  • Sim SJ, Chang HN, Liu JR, Jung KH (1994) Production and secretion of indole alkaloids in hairy root cultures of Catharanthus roseus: effects of in situ adsorption, fungal elicitation and permeabilization. J Ferment Bioeng 78:229–234

    CAS  Google Scholar 

  • Singh R, Kharb P, Rani K (2011) Rapid micropropagation and callus induction of Catharanthus roseus in vitro using different explants. World J Agric Sci 7:699–704

    Google Scholar 

  • Srivastava T, Das S, Sopory SK, Srivastava PS (2009) A reliable protocol for transformation of Catharanthus roseus through Agrobacterium tumefaciens. Physiol Mol Biol Plants 15:93–98

    CAS  PubMed  PubMed Central  Google Scholar 

  • Swanberg A, Dai W (2008) Plant regeneration of periwinkle (Catharanthus roseus) via organogenesis. Hort Sci 43:832–836

    Google Scholar 

  • Tonk D, Mujib A, Maqsood M, Ali M, Zafar N (2016) Aspergillus flavus fungus elicitation improves vincristine and vinblastine yield by augmenting callus biomass growth in Catharanthus roseus. Plant Cell Tissue Organ Cult 126:291–303

    CAS  Google Scholar 

  • Uniyal GC, Bala S, Mathur AK, Kulkarni RN (2001) Symmetry C18 column: a better choice for the analysis of indole alkaloids of Catharanthus roseus. Phytochem Anal 12:206–210

    CAS  PubMed  Google Scholar 

  • Van der Heijden R, Jacobs DI, Snoeijer W, Hallard D, Verpoorte R (2004) The Catharanthus alkaloids: pharmacognosy and biotechnology. Curr Med Chem 11:607–628

    Google Scholar 

  • Velayutham K, Rahuman AA, Rajakumar G, Santhoshkumar T, Marimuthu S, Jayaseelan C, Bagavan A, Kirthi AV, Kamaraj C, Zahir AA, Elango G (2012) Evaluation of Catharanthus roseus leaf extract-mediated biosynthesis of titanium dioxide nanoparticles against Hippobosca maculata and Bovicola ovis. Parasitol Res 111:2329–2337

    PubMed  Google Scholar 

  • Verma P, Mathur AK (2011) Direct shoot bud organogenesis and plant regeneration from pre-plasmolysed leaf explants in Catharanthus roseus. Plant Cell, Tissue and Organ Cult 106:401–408

    CAS  Google Scholar 

  • Verma P, Mathur AK, Shanker K (2012) Growth, alkaloid production, rol genes integration, bioreactor up-scaling and plant regeneration studies in hairy root lines of Catharanthus roseus. Plant Biosyst 146:27–40

    Google Scholar 

  • Verma P, Sharma A, Khan SA, Mathur AK, Shanker K (2015) Morphogenetic and chemical stability of long-term maintained -mediated transgenic plants. Nat Prod Res 29:315–320

  • Xiang B, Zhu R, Wang W, Bai Y, Wang Y (2010) Cell line screening of Catharanthus roseus for high yield production of ajmalicine. J Med Plant Res 5:420–424

    Google Scholar 

  • Xing S, Guo X, Wang Q, Pan Q, Tian Y, Liu P, Zhao J, Wang G, Sun X, Tang K (2011) Induction and flow cytometry identification of tetraploids from seed-derived explants through colchicine treatments in Catharanthus roseus (L.) G. Don. J Biomed Biotechnol 2011:793198

    PubMed  PubMed Central  Google Scholar 

  • Xu M, Dong J (2005a) Nitric oxide stimulates indole alkaloid production in Catharanthus roseus cell suspension cultures through a protein kinase-dependent signal pathway. Enzym Microb Technol 37:49–53

    CAS  Google Scholar 

  • Xu M, Dong J (2005b) O2− from elicitor-induced oxidative burst is necessary for triggering phenylalanine ammonia-lyase activation and catharanthine synthesis in Catharanthus roseus cell cultures. Enzym Microb Technol 36:280–284

    CAS  Google Scholar 

  • Xu M, Dong J, Zhu M (2005) Effect of nitric oxide on catharanthine production and growth of Catharanthus roseus suspension cells. Biotechnol Bioeng 89:367–371

    CAS  PubMed  Google Scholar 

  • Yuan YJ, Hu TT, Yang YM (1994) Effects of auxins and cytokinins on formation of Catharanthus roseus G. Don multiple shoots. Plant Cell Tissue Organ Cult 37:193–196

    CAS  Google Scholar 

  • Yuan F, Wang Q, Pan Q, Wang G, Zhao J, Tian Y, Tang K (2011) An efficient somatic embryogenesis based plant regeneration from the hypocotyl of Catharanthus roseus. Afr J Biotechnol 10:14786–14795

    CAS  Google Scholar 

  • Zárate R, Memelink J, van der Heijden R, Verpoorte R (1999) Genetic transformation via particle bombardment of Catharanthus roseus plants through adventitious organogenesis of buds. Biotechnol Lett 21:997–1002

    Google Scholar 

  • Zhao J, Zhu WH, Hu Q (2000a) Promotion of indole alkaloid production in Catharanthus roseus cell cultures by rare earth elements. Biotechnol Lett 22:825–828

    CAS  Google Scholar 

  • Zhao J, Zhu WH, Hu Q, Guo YQ (2000b) Improvement of indole alkaloid production in Catharanthus roseus cell cultures by osmotic shocks. Biotechnol Lett 22:1227–1231

    CAS  Google Scholar 

  • Zhao J, Hu Q, Guo YQ, Zhu WH (2001a) Effects of stress factors, bioregulators, and synthetic precursors on indole alkaloid production in compact callus clusters cultures of Catharanthus roseus. Appl Microbiol Biotechnol 55:693–698

    CAS  PubMed  Google Scholar 

  • Zhao J, Zhu WH, Hu Q (2001b) Effects of light and plant growth regulators on the biosynthesis of vindoline and other indole alkaloids in Catharanthus roseus callus cultures. Plant Growth Regul 33:43–49

    CAS  Google Scholar 

  • Zhao J, Zhu WH, Hu Q (2001c) Enhanced catharanthine production in Catharanthus roseus cell cultures by combined elicitor treatment in shake flasks and bioreactors. Enzym Microb Technol 28:673–681

    CAS  Google Scholar 

  • Zhao J, Zhu WH, Hu Q, Guo YQ (2001d) Compact callus cluster suspension cultures of Catharanthus roseus with enhanced indole alkaloid biosynthesis. In Vitro Cell Dev Bio—Plant 37:68–72

    CAS  Google Scholar 

  • Zhao J, Zhu WH, Hu Q, He XW (2001e) Enhanced indole alkaloid production in suspension compact callus clusters of Catharanthus roseus: impacts of plant growth regulators and sucrose. Plant Growth Regul 33:33–41

    Google Scholar 

Download references

Acknowledgments

Authors acknowledge the University e-library assistance and the experimental assistance from Plant Tissue Culture laboratory at Regional Nuclear Agricultural Research Centre, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India. We are further thankful to the anonymous reviewer(s) and the editor of this article for their critical comments and suggestions on the manuscript.

Author information

Authors and Affiliations

  1. Crop Research Unit (Genetics and Plant Breeding), Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, 741252, India

    Anamika Das, Sutanu Sarkar, Somnath Bhattacharyya & Saikat Gantait

Authors
  1. Anamika Das
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sutanu Sarkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Somnath Bhattacharyya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Saikat Gantait
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

SG conceived the idea of the review. AD and SG surveyed the literature and drafted the initial manuscript. SG, SS, and SB scrutinized and corrected the manuscript to its final version. All the authors read and approved the final version of the manuscript prior to its submission.

Corresponding author

Correspondence to Saikat Gantait.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Das, A., Sarkar, S., Bhattacharyya, S. et al. Biotechnological advancements in Catharanthus roseus (L.) G. Don. Appl Microbiol Biotechnol 104, 4811–4835 (2020). https://doi.org/10.1007/s00253-020-10592-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-020-10592-1

Keywords

Search

Navigation