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Yeast metabolic engineering for the production of pharmaceutically important secondary metabolites

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Abstract

In the last few decades, there has been a rapid increase in the discovery of drugs from natural products, particularly secondary metabolites. Various efforts have been undertaken to enhance and optimize the production system of these secondary metabolites to meet the increasing global market demand. Recently, metabolic engineering has been used for the heterologous synthesis of secondary metabolites in the engineered yeast strains. Here, we highlight the recent advancements in the production of pharmaceutically important secondary metabolites in metabolically engineered yeast, such as Saccharomyces cerevisiae and Pichia pastoris. Furthermore, we also emphasize the important application of synthetic biology methods that are supported by state-of-the-art post-genomic tools to improve the efficiency and success rate of yeast metabolic engineering for the production of natural drugs. Metabolic engineering using yeast as a microbial host factory to produce pharmaceutically useful secondary metabolites is a very promising strategy, which can be used to support the industrial production system.

Key points

•Next-generation sequencing application for genome mining of secondary metabolites

•Various synthetic biology tools for yeast metabolic engineering construction

•Examples of successfully produced medicinal secondary metabolites in engineered yeast

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Funding

This work was supported by the Development of Foundational Techniques for the Domestic Production of Herbal Medicines (K18405), Applicational Development of Standardized Herbal Resources (KSN1911420), Development of Sustainable Application for Standard Herbal Resources (KSN2012320), Korea Institute of Oriental Medicine through the Ministry of Science and ICT, Republic of Korea.

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  1. Campus of Korea Institute of Oriental Medicine, Korean Convergence Medicine Major, University of Science & Technology (UST), Daejeon, 34054, Republic of Korea

    Endang Rahmat & Youngmin Kang

  2. Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111 Geonjae-ro, Naju-si, 58245, Jeollanam-do, Republic of Korea

    Endang Rahmat & Youngmin Kang

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ER contributes for acquisition of data and writing of manuscript. YMK supports the conception, design strategies, and revising for essential intellectual content of this manuscript.

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Rahmat, E., Kang, Y. Yeast metabolic engineering for the production of pharmaceutically important secondary metabolites. Appl Microbiol Biotechnol 104, 4659–4674 (2020). https://doi.org/10.1007/s00253-020-10587-y

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