The effect of plant compartments on the Broussonetia papyrifera-associated fungal and bacterial communities

Chen, Peilin; Zhao, Meilin; Tang, Feng; Hu, Yanmin; Peng, Xianjun; Shen, Shihua

doi:10.1007/s00253-020-10466-6

Environmental biotechnology
Published: 20 February 2020

The effect of plant compartments on the Broussonetia papyrifera-associated fungal and bacterial communities

Peilin Chen^1,2,
Meilin Zhao^1,2,
Feng Tang^1,2,
Yanmin Hu^1,2,
Xianjun Peng^nAff1 &
Shihua Shen¹

Applied Microbiology and Biotechnology volume 104, pages 3627–3641 (2020)Cite this article

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Abstract

Plants associate with numerous microbes, but little is known about how microbiome components, especially fungi, adapt to specific plant compartments. The adaptability of microbial function to the plant compartment is also not clear especially for woody species. Here, we characterized the bacterial and fungal communities in root endosphere, stems, and rhizospheres of 33 Broussonetia papyrifera seedlings, based on amplification of 16S and ITS rRNA. Results showed that the α-diversity indexes of the bacterial community were significantly different in different plant compartments and they significantly increased from stem to root endosphere to the rhizosphere, whereas those of the fungal community were similar (p > 0.05). However, the result of constrained PCoA (CPCoA) and analysis of similarity (ANOSIM) showed that both bacterial and fungal compositions were significantly affected by plant compartments (p < 0.01). In detail, the operational taxonomic units (OTUs) distribution of the bacterial community was significantly different, but 249 of 252 fungal OTUs were shared in different plant compartments. Both the bacterial and fungal compositions were significantly influenced by plant compartments, based on the result on phyla, core OTUs, and indicator OTUs level. Further, 40 of 42 enriched KEGG pathways involving the bacteria also differed significantly among plant compartments (p < 0.01). This study provides an understanding of the influence of plant compartments on the microbiome and confirms that the disperse limitation of fungal OTUs across different plant compartments is smaller. This study sheds light on how the microbial community adapts to and thrives in different plant compartments.

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Acknowledgments

We thank Zhi Pi, Jinshan Wang and Jie Hu for planting B. papyrifera and collecting samples.

Funding

This work was supported by the National Natural Science Foundation of China (31770360, 31870247), the Poverty Relief Project of the Chinese Academy of Sciences (KFJ-FP-24), and the Huimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City (Z151100001015008).

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Xianjun Peng
Present address: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

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Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China
Peilin Chen, Meilin Zhao, Feng Tang, Yanmin Hu & Shihua Shen
University of Chinese Academy of Sciences, Beijing, 100049, China
Peilin Chen, Meilin Zhao, Feng Tang & Yanmin Hu

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Peilin Chen
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Meilin Zhao
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Feng Tang
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Yanmin Hu
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Xianjun Peng
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Shihua Shen
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Chen, P., Zhao, M., Tang, F. et al. The effect of plant compartments on the Broussonetia papyrifera-associated fungal and bacterial communities. Appl Microbiol Biotechnol 104, 3627–3641 (2020). https://doi.org/10.1007/s00253-020-10466-6

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Received: 29 October 2019
Revised: 21 January 2020
Accepted: 12 February 2020
Published: 20 February 2020
Issue Date: April 2020
DOI: https://doi.org/10.1007/s00253-020-10466-6

Keywords

B. papyrifera
Plant compartment
Fungal community
Bacterial community
Predicted function