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Isolation and identification of new antibacterial compounds from Bacillus pumilus

Applied Microbiology and Biotechnology volume 103, pages 8375–8381 (2019)Cite this article

Abstract

Because of the emergence of antibiotic resistance, we must investigate new antibiotical agents. The present study was designed to find new compounds with antibacterial activity from metabolites of Bacillus pumilus. We found that the concentrated fermentation broth of Bacillus pumilus has antibacterial property. By high-performance liquid chromatography (HPLC), three compounds with antibacterial activity were first isolated from the ethyl acetate layer of fermentation broth of Bacillus pumilus. And then their structures were identified by nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. According to the data, the compound 1, compound 2, and compound 3 were determined to be 3,4-dipentylhexane-2,5-diol, 1,1′-(4,5-dibutylcyclohexane-1,2-diyl)bis(ethan-1-ol), and 1,1′-(4,5-dibutyl-3,6-dimethylcyclohexane-1,2-diyl)bis(ethan-1-one). And all of them exhibited potent inhibitory effects against a panel of pathogenic bacteria including Staphylococcus aureus ATCC6538, Micrococcus luteus CMCC28001, Variant Salmonella gallinarum CVCC79207, Pasteurella multocida CVCC474, Swine Salmonella, Salmonella enterica ATCC13076, Swine Escherichia coli K88, Chicken Escherichia coli O78. Given its antibacterial activity, 3,4-dipentylhexane-2,5-diol, 1,1′-(4,5-dibutylcyclohexane-1,2-diyl)bis(ethan-1-ol), 1,1′-(4,5-dibutyl-3,6-dimethylcyclohexane-1,2-diyl)bis(ethan-1-one) are assumed to be promising agents for further development as antibacterial agents.

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References

  • Abriouel H, Franz CMAP, Omar NB, Gálvez A (2011) Diversity and applications of Bacillus bacteriocins. FEMS Microbiol Rev 35(1):201–232

    CAS  Article  PubMed  Google Scholar 

  • Alanis AJ (2005) Resistance to antibiotics: are we in the post-antibiotic era? Arch Med Res 36:697–705

    Article  PubMed  Google Scholar 

  • Ali SM, Siddiqui R, Ong SK, Shah MR, Anwar A, Heard PJ, Khan NA (2017) Identification and characterization of antibacterial compound(s) of cockroaches (Periplaneta americana). Appl Microbiol Biotechnol 101:253–286

    CAS  Article  PubMed  Google Scholar 

  • Alvarez F, Castro M, Principe A, Borioli G, Fischer S, Mori G, Jofre E (2012) The plant-associated Bacillus amyloliquefaciens strains MEP2 18 and ARP2 3 capable of producing the cyclic lipopeptides iturin or surfactin and fengycin are effective in biocontrol of sclerotinia stem rot disease. J Appl Microbiol 112:159–174

    CAS  Article  PubMed  Google Scholar 

  • Barth H, Aktories K, Popoff MR, Stiles BG (2004) Binary bacterial toxins: biochemistry, biology, and applications of common Clostridium and Bacillus proteins. Microbiol Mol Biol R 68(3):373–402

    CAS  Article  Google Scholar 

  • Beltran G, Novo M, Guillamón JM, Mas A, Rozès N (2008) Effect of fermentation temperature and culture media on the yeast lipid composition and wine volatile compounds. Int J Food Microbiol 121:169–177

    CAS  Article  PubMed  Google Scholar 

  • Bernhard K, Schrempf H, Goebel W (1978) Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis. J Bacteriol 133(2):897–903

    CAS  PubMed  PubMed Central  Google Scholar 

  • Clardy J, Fischbach MA, Walsh CT (2006) New antibiotics from bacterial natural products. Nat Biotechnol 24:1541–1550

    CAS  Article  PubMed  Google Scholar 

  • Cotter PD, Ross RP, Hill C (2013) Bacteriocins - a viable alternative to antibiotics? Nat Rev Microbiol 11:95–105

    CAS  Article  PubMed  Google Scholar 

  • Devasahayam G, Scheld WM, Hoffman PS (2010) Newer antibacterial drugs for a new century. Expert Opin Invest Drugs 19:215–234

    CAS  Article  Google Scholar 

  • He R (2011) Application effect and method of Bacillus probioticus in livestock and poultry culture. Breed Sci 02:4–5

    Google Scholar 

  • Jagath L, Kadurugamuwa, Beveridge TJ (1996) Bacteriolytic effect of membrane vesicles from Pesudomonas aeruginosa on other bacteia including pathogens: conceptually new antibiotics. J Bacteriol 178(10):2767–2774

    Article  Google Scholar 

  • Kopp BJ, Nix DE, Armstrong EP (2004) Clinical and economic analysis of methicillin-susceptible and -resistant Staphylococcus aureus infections. Ann Pharmacother 38:1377–1382

    Article  PubMed  Google Scholar 

  • Kumarasamy KK, Toleman MA, Walsh TR, Bagaria J, Butt F, Balakrishnan R, Chaudhary U, Doumith M, Giske CG, Irfan S, Krishnan P, Kumar AV, Maharjan S, Mushtaq S, Noorie T, Paterson DL, Pearson A, Perry C, Pike R, Rao B, Ray U, Sarma JB, Sharma M, Sheridan E, Thirunarayan MA, Turton J, Upadhyay S, Warner M, Welfare W, Livermore DM, Woodford N (2010) Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis 10:597–602

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Li J, Yang Q, Zhao LH, Zhang SM, Wang YX, Zhao XY (2009) Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29. J Zhejiang Univ Sci B 10:264–272

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Linzenmeier G (1958) Kanamycin. Dtsch Med Wochenschr 83:2298

    Article  PubMed  Google Scholar 

  • Moyne AL, Shelby R, Cleveland TE, Tuzun S (2001) Bacillomycin D: an iturin with antifungal activity against Aspergillus flavus. J Appl Microbiol 90:622–629

    CAS  Article  PubMed  Google Scholar 

  • Nordmann P, Naas T, Fortineau N, Poirel L (2007) Superbugs in the coming new decade; multidrug resistance and prospects for treatment of Staphylococcus aureus, Enterococcus spp. and Pseudomonas aeruginosa in 2010. Curr Opin Microbiol 10:436–440

    CAS  Article  PubMed  Google Scholar 

  • Pueyo MT, Bloch CJ, Carmona-Ribeiro AM, di Mascio P (2009) Lipopeptides produced by a soil Bacillus megaterium strain. Microb Ecol 57:367–378

    CAS  Article  PubMed  Google Scholar 

  • Pulaski EJ, Shaeffer JR, Artz CP, Baker HJ (1950) Studies on the use of terramycin in experimental and clinical peritonitis. Surg Forum 1950:119–129

    Google Scholar 

  • Ramarathnam R, Bo S, Chen Y, Fernando WG, Xuewen G, de Kievit T (2007) Molecular and biochemical detection of fengycin- and bacillomycin D-producing Bacillus spp., antagonistic to fungal pathogens of canola and wheat. Can J Microbiol 53:901–911

    CAS  Article  PubMed  Google Scholar 

  • Schatz A, Bugie E, Waksman SA (2005) Streptomycin, a substance exhibiting antibiotic activity against gram-positive and gram-negative bacteria. 1944. Clin Orthop Relat Res 3–6, &NA

  • Sidorova TM, Asaturova AM, Homyak AI (2018) Biologically active metabolites of Bacillus subtilis and their role in the control of phytopathogenic microorganisms. Agr Biol 53(1):29–37

    Google Scholar 

  • Tatum AL (1948) Chloromycetin; an antibiotic effective in the treatment of rickettsial diseases. Wis Med J 47:697

    CAS  PubMed  Google Scholar 

  • Zeilinger S, Galhaup C, Payer K, Woo SL, Mach RL, Fekete C, Lorito M, Kubicek CP (1999) Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genet Biol 26:131–140

    CAS  Article  PubMed  Google Scholar 

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Funding

This study was funded by the National Key Research and Development Plan of China (grant number: 2016YFE0111600) and the Collaborative Innovation Fund of Shandong Academy of Sciences (grant number: 2017CXY-17).

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Affiliations

  1. Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), 28789 East Jingshi Road, Ji’nan, 250103, Shandong, China

    Jie Chu, Ying Wang, Bo Zhao, Xuan-ming Zhang & Kechun Liu

  2. College of Animal Science and Technology of Shandong Agricultural University, Tai’an, 271018, Shandong, China

    Linjing Mao

  3. Institute of Microbiology, National Academy of Sciences, Belarus, Kuprevich Str. 2, 220141, Minsk, Belarus

    Emiliya Kalamiyets

Authors
  1. Jie Chu
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  2. Ying Wang
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  3. Bo Zhao
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  4. Xuan-ming Zhang
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  5. Kechun Liu
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  6. Linjing Mao
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  7. Emiliya Kalamiyets
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Correspondence to Jie Chu.

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Chu, J., Wang, Y., Zhao, B. et al. Isolation and identification of new antibacterial compounds from Bacillus pumilus. Appl Microbiol Biotechnol 103, 8375–8381 (2019). https://doi.org/10.1007/s00253-019-10083-y

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  • DOI: https://doi.org/10.1007/s00253-019-10083-y

Keywords

  • Antibacterial compounds
  • Bacillus pumilus
  • Fermentation
  • Identification