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Dynamics and potential roles of abundant and rare subcommunities in the bioremediation of cadmium-contaminated paddy soil by Pseudomonas chenduensis

Applied Microbiology and Biotechnology volume 103, pages 8203–8214 (2019)Cite this article

Abstract

Microbial bioremediation of heavy metal–contaminated soil is a potential technique to reduce heavy metals in crop plants. However, the dynamics and roles of the local microbiota in bioremediation of heavy metal–contaminated soil following microbial application are rarely reported. In this study, we used Pseudomonas chenduensis strain MBR for bioremediation of Cd-contaminated paddy soil and investigated its effects on the dynamics of the local soil bacterial community and Cd accumulation in rice. Cd accumulation in rice grains and roots were significantly reduced by the addition of the strain MBR. The addition of the strain MBR caused greater changes in bacterial communities in rhizosphere soil than in bulk soil. MBR enhanced the roles of microbial communities in transformation of Cd fractions, especially in rhizosphere soil. The strain MBR likely regulated abundant subcommunities more than rare subcommunities to improve Cd bioremediation, especially in rhizosphere soil. Consequently, the dynamics and functional roles of the local microbial communities differed significantly during bioremediation between abundant and rare subcommunities and between rhizosphere soil and bulk soil. This study provides new insight into the microbiota-related mechanisms underlying bioremediation.

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Funding

The study was funded by the National Key Research and Development Program of China (2016YFD0800702) and the Open Foundation Project of Key Laboratory of Environmental and Applied Microbiology, CAS (KLCAS-2018-4), Sichuan Key Research and Development Program (2017SZ0184), and China Biodiversity Observation Networks (Sino BON).

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Author notes

  1. Lingjuan Li and Qiang Lin contributed equally to this work.

Affiliations

  1. Key Laboratory of Environmental and Applied Microbiology, CAS & Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China

    Lingjuan Li, Xiangzhen Li, Tiezhu Li, Xiaohong He, Daping Li & Yong Tao

  2. Biology Centre of the Czech Academy of Sciences, Institute of Soil Biology & SoWa Research Infrastructure, Na Sádkách 7, 37005, České Budějovice, Czech Republic

    Qiang Lin

Authors
  1. Lingjuan Li
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  2. Qiang Lin
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  3. Xiangzhen Li
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  4. Tiezhu Li
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  5. Xiaohong He
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  6. Daping Li
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  7. Yong Tao
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Contributions

Lingjuan Li (LL) and Qiang Lin (QL) performed the experiments, analyzed the results, and wrote the manuscript. Yong Tao (YT) conceived and designed the study, as well as revised the manuscript. Xiangzhen Li (XL) revised the manuscript. Tiezhu Li (TL), Xiaohong He (XH), and Daping Li (DL) participated in the study. All authors read and approved the final manuscript.

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Correspondence to Yong Tao.

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Li, L., Lin, Q., Li, X. et al. Dynamics and potential roles of abundant and rare subcommunities in the bioremediation of cadmium-contaminated paddy soil by Pseudomonas chenduensis. Appl Microbiol Biotechnol 103, 8203–8214 (2019). https://doi.org/10.1007/s00253-019-10059-y

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  • DOI: https://doi.org/10.1007/s00253-019-10059-y

Keywords

  • Cadmium contamination
  • Paddy soil
  • Pseudomonas chenduensis
  • Abundant and rare bacteria