Abstract
Microbial metagenome analysis has proven its usefulness to investigate the microbiomes present in technical engineered ecosystems such as anaerobic digestion systems. The analysis of the total microbial genomic DNA allows the detailed determination of both the microbial community structure and its functionality. In addition, it enables to study the response of the microbiome to alterations in technical process parameters. Strategies of functional microbial networks to face abiotic stressors, e.g., resistance, resilience, and reorganization, can be evaluated with respect to overall process optimization. The objective of this paper is to review the main metagenomic tools used for effective studies on anaerobic digestion systems in monitoring the dynamic of the microbiomes, as well as the factors that have been identified so far as limiting the metagenomic studies in this ecosystems.
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09 November 2019
The original version of this article was revised: After publication of this article, the publisher was notified that Michael Klocke has been listed as an author without his consent.
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This paper is a result of cooperation between the Cuban and German institutions involved, and it was supported by the postdoctoral fellowship grant of Janet Jiménez (Grant No: 91663901), provided by the German Academic Exchange Service (DAAD).
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Carabeo-Pérez, A., Guerra-Rivera, G., Ramos-Leal, M. et al. Metagenomic approaches: effective tools for monitoring the structure and functionality of microbiomes in anaerobic digestion systems. Appl Microbiol Biotechnol 103, 9379–9390 (2019). https://doi.org/10.1007/s00253-019-10052-5
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DOI: https://doi.org/10.1007/s00253-019-10052-5