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Enhancement of antroquinonol and antrodin C productions via in situ extractive fermentation of Antrodia camphorata S-29

  • Biotechnological products and process engineering
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Abstract

This study describes the application of in situ extractive fermentation (ISEF) to increase the yields of antroquinonol (AQ) and antrodin C (AC) from Antrodia camphorata S-29. In initial screening experiments, nine solvents were tested to identify the most suitable extractant for the in situ extraction of AQ and AC. These solvents included n-tetradecane, n-dodecane, n-decane, heavy paraffin, light paraffin, oleyl alcohol, oleic acid, butyl oleate, and isopropyl myristate. Of these, oleic acid was the most suitable solvent for the in situ extraction of AQ and AC. The use of oleic acid as an in situ extractant significantly improved AQ and AC productions, which were approximately 5-fold and 8-fold that of the control, respectively. The recovered oleic acid was treated with a silica gel solid-phase extraction column, which was able to rapidly adsorb the bioactive metabolites. The separated solvent hardly contained fermentation products and could be directly reused in ISEF. AQ and AC were obtained with purities of over 75% by silica gel column chromatography. The recoveries of AQ and AC reached 70.7 ± 0.8% and 81.5 ± 1.2%, respectively.

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Funding

This work was supported by the National Natural Science Foundation of China (grant number 31871757) and Shanghai Engineering Research Center of Food Microbiology (grant number 19 DZ2281100).

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Authors and Affiliations

  1. Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, People’s Republic of China

    Xiaofeng Liu, Yongjun Xia, Yao Zhang, Zhenwei Yi, Peng Meng, Guangqiang Wang & Lianzhong Ai

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  1. Xiaofeng Liu
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  2. Yongjun Xia
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  3. Yao Zhang
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  4. Zhenwei Yi
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  5. Peng Meng
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  6. Guangqiang Wang
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  7. Lianzhong Ai
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Correspondence to Lianzhong Ai.

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Liu, X., Xia, Y., Zhang, Y. et al. Enhancement of antroquinonol and antrodin C productions via in situ extractive fermentation of Antrodia camphorata S-29. Appl Microbiol Biotechnol 103, 8351–8361 (2019). https://doi.org/10.1007/s00253-019-10034-7

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