Applied Microbiology and Biotechnology

, Volume 103, Issue 17, pp 7129–7140 | Cite as

Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79

  • Zhiyong Zhao
  • Yanmei Zhang
  • Andong Gong
  • Na Liu
  • Shanshan Chen
  • Xiaoyan Zhao
  • Xiaobei Li
  • Lei Chen
  • Changyan Zhou
  • Jianhua WangEmail author
Applied microbial and cell physiology


Fumonisin B1 (FB1) contamination in cereals and cereal products remains an important aspect of food safety because of its wide distribution and the potential health hazard. However, only a few microorganisms have been reported to effectively degrade FB1. In this present study, a bacterial consortium SAAS79 with highly FB1-degrading activity was isolated from the spent mushroom compost. The combination of antibiotic-driven selection and 16S rDNA sequencing identified the Pseudomonas genus as the key FB1-degrading member. The microbial consortium could degrade more than 90% of 10 μg/mL FB1 after incubation for 24 h at pH of 5–7 and temperature of 28–35 °C. The enzymes from the intracellular space were proved to be responsible for FB1 degradation, which eliminated about 90% of 10 μg/mL FB1 in 3 h. Besides, liquid chromatography time-of-flight mass spectrometry (LC-TOF/MS) analysis identified two degradation products of FB1, and their toxicity on the monkey kidney cells (MARC-145) was significantly lower (p < 0.05) compared with the parent FB1. Overall, the consortium SAAS79 and its crude enzymes may be a potential choice for the decontamination of FB1 in the feed and food industry. Also, the bacterial consortium provides a new source of genes for the development of enzymatic detoxification agent.


Biodegradation Fumonisin B1 Bacterial consortium Enzymatic detoxification Metabolites 



The authors acknowledged the financial support from National Natural Science Foundation of China (31602124, 31871896, and 31401598) and Shanghai Agriculture Commission Basic Research Project (grant number 2014 no. 7-3-7).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2019_9979_MOESM1_ESM.pdf (38 kb)
ESM 1 (PDF 37 kb)


  1. Adebo OA, Njobeh PB, Sidu S, Adebiyi JA, Mavumengwana V (2017) Aflatoxin B1 degradation by culture and lysate of a Pontibacter specie. Food Control 80:99–103. CrossRefGoogle Scholar
  2. Alberts JF, van Zyl WH, Gelderblom WCA (2016) Biologically based methods for control of fumonisin-producing Fusarium species and reduction of the fumonisins. Front Microbiol 7(548):1–33. CrossRefGoogle Scholar
  3. Bartók T, Tölgyesi L, Szekeres A, Varga M, Bartha R, Szécsi Á, Bartók M, Mesterházy Á (2010) Detection and characterization of twenty-eight isomers of fumonisin B1 (FB1) mycotoxin in a solid rice culture infected with Fusarium verticillioides by reversed-phase high-performance liquid chromatography/electrospray ionization time-of-flight and ion trap mass spectrometry. Rapid Commun Mass Spectrom 24(1):35–42. CrossRefPubMedGoogle Scholar
  4. Benedetti R, Nazzi F, Locci R, Firrao G (2006) Degradation of fumonisin B1 by a bacterial strain isolated from soil. Biodegradation 17(1):31–38. CrossRefPubMedGoogle Scholar
  5. Bennett GA, Richard JL, Eckhoff SR (1996) Distribution of fumonisins in food and feed products prepared from contaminated corn. In: Jackson LS, DeVries JW, Bullerman LB (eds) Fumonisins in food. Springer, Boston, MA, pp 317–322CrossRefGoogle Scholar
  6. Blackwell BA, Gilliam JT, Savard ME, David Miller J, Duvick JP (1999) Oxidative deamination of hydrolyzed fumonisin B1 (AP1) by cultures of Exophiala spinifera. Nat Toxins 7(1):31–38.<31::AID-NT36>3.0.CO;2-W CrossRefPubMedGoogle Scholar
  7. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254. CrossRefGoogle Scholar
  8. Braun MS, Wink M (2018) Exposure, occurrence, and chemistry of fumonisins and their cryptic derivatives. Compr Rev Food Sci Food Saf 17(3):769–791. CrossRefGoogle Scholar
  9. Canela R, Pujol R, Sala N, Sanchis V (1996) Fate of fumonisins B1 and B2 in steeped corn kernels. Food Addit Contam 13(5):511–517. CrossRefPubMedGoogle Scholar
  10. Duvick J, Rood T, Maddox J, Gilliam J (1998) Detoxification of mycotoxins in planta as a strategy for improving grain quality and disease resistance: identification of fumonisin-degrading microbes from maize. In: Kohmoto K, Yoder OC (eds) Molecular genetics of host-specific toxins in plant disease, developments in plant pathology, vol 13. Springer, Dordrecht, pp 369–381CrossRefGoogle Scholar
  11. EC (2006) Commission Recommendation of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding (2006/576/EC). Off J Euro Union 229:7–9. Accessed 18 June 2019Google Scholar
  12. EC (2007) Commission Regulation (EC) No 1126/2007 of 28 September 2007 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards Fusarium toxins in maize and maize products. Off J Euro Union 255:14–17. Accessed 18 June 2019Google Scholar
  13. EFSA (2014) Scientific opinion on the safety and efficacy of fumonisin esterase (FUMzyme®) as a technological feed additive for pigs. EFSA J 12(5):3667. CrossRefGoogle Scholar
  14. EFSA (2016) Safety and efficacy of fumonisin esterase (FUMzyme®) as a technological feed additive for all avian species. EFSA J 14(11):e04617. CrossRefGoogle Scholar
  15. EFSA (2018a) Risks for animal health related to the presence of fumonisins, their modified forms and hidden forms in feed. EFSA J 16(5):e05242. CrossRefGoogle Scholar
  16. EFSA (2018b) Safety and efficacy of fumonisin esterase from Komagataella phaffii DSM 32159 as a technological feed additive for pigs and poultry. EFSA J 16(5):e05269. CrossRefGoogle Scholar
  17. FDA (2001) Background paper in support of fumonisin levels in animal feed: executive summary of this scientific support document. Center for Food Safety and Applied Nutrition, Center for Veterinary Medicine. Accessed 18 June 2019
  18. Gelderblom W, Jaskiewicz K, Marasas W, Thiel P, Horak R, Vleggaar R, Kriek N (1988) Fumonisins-novel mycotoxins with cancer-promoting activity produced by Fusarium moniliforme. Appl Environ Microbiol 54(7):1806–1811PubMedPubMedCentralGoogle Scholar
  19. Grenier B, Bracarense APF, Schwartz HE, Trumel C, Cossalter AM, Schatzmayr G, Kolf-Clauw M, Moll WD, Oswald IP (2012) The low intestinal and hepatic toxicity of hydrolyzed fumonisin B1 correlates with its inability to alter the metabolism of sphingolipids. Biochem Pharmacol 83(10):1465–1473. CrossRefPubMedGoogle Scholar
  20. Gu MJ, Han SE, Hwang K, Mayer E, Reisinger N, Schatzmayr D, Park BC, Han SH, Yun CH (2019) Hydrolyzed fumonisin B1 induces less inflammatory responses than fumonisin B1 in the co-culture model of porcine intestinal epithelial and immune cells. Toxicol Lett 305:110–116. CrossRefPubMedGoogle Scholar
  21. Hahn I, Nagl V, Schwartz-Zimmermann HE, Varga E, Schwarz C, Slavik V, Reisinger N, Malachová A, Cirlini M, Generotti S (2015) Effects of orally administered fumonisin B1 (FB1), partially hydrolysed FB1, hydrolysed FB1 and N-(1-deoxy-D-fructos-1-yl) FB1 on the sphingolipid metabolism in rats. Food Chem Toxicol 76:11–18. CrossRefPubMedGoogle Scholar
  22. He WJ, Yuan QS, Zhang YB, Guo MW, Gong AD, Zhang JB, Wu AB, Huang T, Qu B, Li HP (2016) Aerobic de-epoxydation of trichothecene mycotoxins by a soil bacterial consortium isolated using in situ soil enrichment. Toxins 8(10):277. CrossRefPubMedCentralGoogle Scholar
  23. Heinl S, Hartinger D, Thamhesl M, Schatzmayr G, Moll WD, Grabherr R (2011) An aminotransferase from bacterium ATCC 55552 deaminates hydrolyzed fumonisin B1. Biodegradation 22(1):25–30. CrossRefPubMedGoogle Scholar
  24. Heinl S, Hartinger D, Thamhesl M, Vekiru E, Krska R, Schatzmayr G, Moll WD, Grabherr R (2010) Degradation of fumonisin B1 by the consecutive action of two bacterial enzymes. J Biotechnol 145(2):120–129. CrossRefPubMedGoogle Scholar
  25. IARC (2002) Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. IARC Monogr Eval Carcinog Risks Hum 82, IARC Press, Lyon, FranceGoogle Scholar
  26. JECFA (2002) Evaluation of certain mycotoxins in food: Fifty-sixth report of the Joint FAO/WHO Expert Committee on Food Additives. World Health Organization, GenevaGoogle Scholar
  27. Katta S, Cagampang A, Jackson L, Bullerman L (1997) Distribution of Fusarium molds and fumonisins in dry-milled corn fractions. Cereal Chem 74(6):858–863. CrossRefGoogle Scholar
  28. Lee HJ, Ryu D (2017) Worldwide occurrence of mycotoxins in cereals and cereal-derived food products: public health perspectives of their co-occurrence. J Agric Food Chem 65(33):7034–7051. CrossRefPubMedGoogle Scholar
  29. Loi M, Fanelli F, Liuzzi VC, Logrieco AF, Mulè G (2017) Mycotoxin biotransformation by native and commercial enzymes: present and future perspectives. Toxins 9(4):111. CrossRefPubMedCentralGoogle Scholar
  30. Loiseau N, Polizzi A, Dupuy A, Therville N, Rakotonirainy M, Loy J, Viadere J-L, Cossalter AM, Bailly JD, Puel O (2015) New insights into the organ-specific adverse effects of fumonisin B1: comparison between lung and liver. Arch Toxicol 89(9):1619–1629. CrossRefPubMedGoogle Scholar
  31. Masching S, Naehrer K, Schwartz-Zimmermann HE, Sărăndan M, Schaumberger S, Dohnal I, Nagl V, Schatzmayr D (2016) Gastrointestinal degradation of fumonisin B1 by carboxylesterase FumD prevents fumonisin induced alteration of sphingolipid metabolism in Turkey and swine. Toxins 8(3):84. CrossRefPubMedCentralGoogle Scholar
  32. Munkvold GP, Arias S, Taschl I, Gruber-Dorninger C (2019) Mycotoxins in corn: occurrence, impacts, and management. In: Sergio OSS (ed) Corn:chemistry and technology, 3rd edn. AACC International, Eagan, Minnesota, pp 235–287CrossRefGoogle Scholar
  33. Palencia E, Torres O, Hagler W, Meredith FI, Williams LD, Riley RT (2003) Total fumonisins are reduced in tortillas using the traditional nixtamalization method of Mayan communities. J Nutr 133(10):3200–3203. CrossRefPubMedGoogle Scholar
  34. Park DL, López-García R, Trujillo-Preciado S, Price RL (1996) Reduction of risks associated with fumonisin contamination in corn. In: Jackson LS, DeVries JW, Bullerman LB (eds) Fumonisins in Food. Springer, Boston, MA, pp 335–344CrossRefGoogle Scholar
  35. Sangare L, Zhao Y, Folly Y, Chang J, Li J, Selvaraj J, Xing F, Zhou L, Wang Y, Liu Y (2014) Aflatoxin B1 degradation by a Pseudomonas strain. Toxins 6(10):3028–3040. CrossRefPubMedPubMedCentralGoogle Scholar
  36. Schertz H, Dänicke S, Frahm J, Schatzmayr D, Dohnal I, Bichl G, Schwartz-Zimmermann H, Colicchia S, Breves G, Teifke J (2018) Biomarker evaluation and toxic effects of an acute oral and systemic fumonisin exposure of pigs with a special focus on dietary fumonisin esterase supplementation. Toxins 10(7):296. CrossRefPubMedCentralGoogle Scholar
  37. Schwartz-Zimmermann H, Hartinger D, Doupovec B, Gruber-Dorninger C, Aleschko M, Schaumberger S, Nagl V, Hahn I, Berthiller F, Schatzmayr D (2018) Application of biomarker methods to investigate FUMzyme mediated gastrointestinal hydrolysis of fumonisins in pigs. World Mycotoxin J 11(2):201–214. CrossRefGoogle Scholar
  38. Scott P, Lawrence G (1994) Stability and problems in recovery of fumonisins added to corn-based foods. J AOAC Int 77(2):541–545PubMedGoogle Scholar
  39. Solfrizzo M, Visconti A, Avantaggiato G, Torres A, Chulze S (2001) In vitro and in vivo studies to assess the effectiveness of cholestyramine as a binding agent for fumonisins. Mycopathologia 151(3):147–153. CrossRefPubMedGoogle Scholar
  40. Streit E, Naehrer K, Rodrigues I, Schatzmayr G (2013) Mycotoxin occurrence in feed and feed raw materials worldwide: long-term analysis with special focus on Europe and Asia. J Sci Food Agric 93(12):2892–2899. CrossRefPubMedGoogle Scholar
  41. Tan H, Zhang Z, Hu Y, Wu L, Liao F, He J, Luo B, He Y, Zuo Z, Ren Z (2015) Isolation and characterization of Pseudomonas otitidis TH-N1 capable of degrading zearalenone. Food Control 47:285–290. CrossRefGoogle Scholar
  42. Völkl A, Vogler B, Schollenberger M, Karlovsky P (2004) Microbial detoxification of mycotoxin deoxynivalenol. J Basic Microbiol 44(2):147–156. CrossRefPubMedGoogle Scholar
  43. Voss K, Smith G, Haschek W (2007) Fumonisins: toxicokinetics, mechanism of action and toxicity. Anim Feed Sci Technol 137(3–4):299–325. CrossRefGoogle Scholar
  44. Wang L, Yue T, Yuan Y, Wang Z, Ye M, Cai R (2015) A new insight into the adsorption mechanism of patulin by the heat-inactive lactic acid bacteria cells. Food Control 50:104–110. CrossRefGoogle Scholar
  45. Wang Y, Zhao C, Zhang D, Zhao M, Zheng D, Lyu Y, Cheng W, Guo P, Cui Z (2017) Effective degradation of aflatoxin B1 using a novel thermophilic microbial consortium TADC7. Bioresour Technol 224:166–173. CrossRefPubMedGoogle Scholar
  46. Yin S, Guo X, Li J, Fan L, Hu H (2016) Fumonisin B1 induces autophagic cell death via activation of ERN1-MAPK8/9/10 pathway in monkey kidney MARC-145 cells. Arch Toxicol 90(4):985–996. CrossRefPubMedGoogle Scholar
  47. Zhang Z, Hou Z, Yang C, Ma C, Tao F, Xu P (2011) Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8. Bioresour Technol 102(5):4111–4116. CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute for Agro-Food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-Products (Shanghai), Ministry of AgricultureShanghai Academy of Agricultural SciencesShanghaiPeople’s Republic of China
  2. 2.College of Life and ScienceXinyang Normal UniversityXinyangPeople’s Republic of China
  3. 3.CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological SciencesUniversity of Chinese Academy of Sciences, Chinese Academy of SciencesHuairouPeople’s Republic of China

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