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Functional response of sediment bacterial community to iron-reducing bioaugmentation with Shewanella decolorationis S12

  • Environmental biotechnology
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Abstract

Bioaugmentation with exogenously functional microbes is a widely used technology in bioengineering and environmental remediation. Generally, the colonization of inoculated bacteria is considered to be the determining factor in technical success. However, increasing reports have shown that bioaugmentation was still effective when the colonization of inoculated bacteria was unsuccessful. Here, an augmentation study with iron-reducing bacteria (IRB, Shewanella decolorationis S12) was conducted in Fe(II)-poor sediments to elucidate the role of exogenously inoculated bacteria for bioaugmentation performance. The results showed that a sufficient amount of IRB inputs enhanced the iron reduction in bioaugmented sediments, even though the exogenous IRB did not colonize after the beginning of the experiment (less than 1% at day 3). The iron reduction function responded to stimulation of the indigenous IRB community such as Clostridium, Cupriavidus, Fervidicella, and Acinetobacter, which comprised less than 1% in the initial sediments. Moreover, compared with microbial community in control sediment, more positive correlations between OTUs were observed for that in S12-added sediments upon network analysis. The pH and oxidation-reduction potential of sediment were found to be the predominant factors shaping the iron-reducing microbial communities. It meant that exogenous IRB successfully trigged functional community via altering microenvironment by the inoculated bacteria. Overall, this study provides a new insight into the understanding of the role of single strain addition in iron-reducing bioaugmentation.

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Funding

This study was funded by the National Natural Science Foundation of China (No. 91851202, 51508111, 51678163), Doctoral Scientific Research Foundation of East China University of Technology (No. DHBK2017143), and Pearl River S&T Nova Program of Guangzhou, China (No. 201806010050).

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Authors and Affiliations

  1. Jiangxi Province Key Laboratory of the Causes and Control of Atmospheric Pollution, East China University of Technology, Nanchang, 330013, China

    Yuanyuan Pan

  2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, 510070, China

    Yuanyuan Pan, Xunan Yang, Guoping Sun & Meiying Xu

  3. State Key Laboratory of Applied Microbiology Southern China, Guangzhou, 510070, China

    Yuanyuan Pan, Xunan Yang, Guoping Sun & Meiying Xu

  4. Guangdong Open Laboratory of Applied Microbiology, No. 100, Xianlie Zhong Road, Guangzhou, 510070, China

    Yuanyuan Pan, Xunan Yang, Guoping Sun & Meiying Xu

  5. Guangdong Envitek Environmental Equipment and Engineering Co. Ltd., Guangzhou, 510507, China

    Xunan Yang

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  1. Yuanyuan Pan
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  2. Xunan Yang
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  3. Guoping Sun
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  4. Meiying Xu
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Contributions

YP designed the study, performed experiments, analyzed the data, and wrote the manuscript; XY analyzed the data and wrote the manuscript; GS and MX revised the manuscript.

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Correspondence to Xunan Yang or Meiying Xu.

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Pan, Y., Yang, X., Sun, G. et al. Functional response of sediment bacterial community to iron-reducing bioaugmentation with Shewanella decolorationis S12. Appl Microbiol Biotechnol 103, 4997–5005 (2019). https://doi.org/10.1007/s00253-019-09816-w

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  • DOI: https://doi.org/10.1007/s00253-019-09816-w

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