The effect of environment on the microbiome associated with the roots of a native woody plant under different climate types in China

Chen, Peilin; Zhao, Meilin; Tang, Feng; Hu, Yanmin; Peng, Xianjun; Shen, Shihua

doi:10.1007/s00253-019-09747-6

Environmental biotechnology
Published: 22 March 2019

The effect of environment on the microbiome associated with the roots of a native woody plant under different climate types in China

Peilin Chen^1,2,
Meilin Zhao^1,2,
Feng Tang^1,2,
Yanmin Hu^1,2,
Xianjun Peng¹ &
Shihua Shen¹

Applied Microbiology and Biotechnology volume 103, pages 3899–3913 (2019)Cite this article

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Abstract

Few studies have investigated the effect of environment on the root-associated microbiome, especially for woody plants in their native environment. The roots and rhizosphere soils of a native woody species (Broussonetia papyrifera) sampled across four different climate types in China were used to elucidate the influence of environment on the root-associated microbiome. Our results showed that the B. papyrifera root-associated microbiome contained abundant Proteobacteria and Basidiomycota, especially Pseudomonas and Rhizobium. The root-associated microbiomes were found to be significantly different under different climate types except for the bacterial community in the rhizosphere, and the proportion of bacterial operational taxonomic units (OTUs) shared among different climate types was lower than that of fungi. More than 50% of the total variance between microbiomes could be explained by 15 environmental factors, six of which, especially soil concentration phosphate and nitrate, had a significant effect. This study provided a comprehensive understanding of the root-associated microbiome of B. papyrifera and further confirmed the effect of environment on the root-associated microbiome of B. papyrifera under different climate types, with some exceptions in the rhizobacterial community and fungal OTUs. Our findings advanced knowledge of the effect of environment through an exploration of environmental factors and found that the nitrogen and phosphorus content represented the key factors.

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Funding

This work was supported by the National Natural Science Foundation of China (31770360, 31870247), the Poverty Relief Project of the Chinese Academy of Sciences (KFJ-FP-24), and the Huimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City (Z151100001015008).

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Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China
Peilin Chen, Meilin Zhao, Feng Tang, Yanmin Hu, Xianjun Peng & Shihua Shen
University of Chinese Academy of Sciences, Beijing, 100049, China
Peilin Chen, Meilin Zhao, Feng Tang & Yanmin Hu

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Peilin Chen
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Meilin Zhao
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Feng Tang
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Yanmin Hu
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Xianjun Peng
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Shihua Shen
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Chen, P., Zhao, M., Tang, F. et al. The effect of environment on the microbiome associated with the roots of a native woody plant under different climate types in China. Appl Microbiol Biotechnol 103, 3899–3913 (2019). https://doi.org/10.1007/s00253-019-09747-6

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Received: 25 January 2019
Revised: 28 February 2019
Accepted: 11 March 2019
Published: 22 March 2019
Issue Date: 02 May 2019
DOI: https://doi.org/10.1007/s00253-019-09747-6

Keywords

Environmental factors
Rhizosphere
Endosphere
Bacteria
Fungi
Broussonetia papyrifera